Phytochemical Screening and in Vitro Antioxidant Activity of Whole Plant Extracts of Sesuvium Portulacastrum L

Abstract

Objectives: The objectives of this study are to screen the phytochemicals, estimate the content of phenolic and flavonoid compounds, and determine the antioxidant capacity of the Sesuvium portulacastrum L. (S. portulacastrum). Methods:The crude bioactives were extracted from the dried powder of S. portulacastrum in an orbital shaker using ethanol, methanol, acetone, hexane, and diethyl ether solvents. Rotaevaporator was used to concentrate the extracts. Total phenolic and flavonoid content was estimated spectrophotometrically using Folin-Ciocalteu and aluminum chloride reagents, respectively. 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-Azinobis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), hydrogen peroxide (H 2 O 2 ), and ferric reducing antioxidant power (FRAP) assays were used to determine the in vitro antioxidant capacity.Results: Phytochemical screening of the extracts showed the presence of major classes of phytochemicals. Total phenolic content (TPC) expressed as gallic acid equivalents (GAE) ranged from 14.42 (ethanol extract) to 54.05 (diethyl ether extract) mg GAE/g dry weight. Total flavonoid content expressed as quercetin equivalents (QE) ranged from 22.03 (hexane extract) to 56.70 (methanol extract) mg QE/g dry weight. Antioxidant activity determined by different assays is highest in diethyl ether extract. A positive correlation (0.7241≤ r ≥0.8419) was found between the TPC and antioxidant activity determined by DPPH, ABTS, and H 2 O 2 scavenging assays. Furthermore, there is a strong correlation (0.722≤ r ≥0.999) between all the pairs of antioxidant assays. Conclusion:Diethyl ether extract showed the highest TPC and antioxidant potential among all the extracts of S. portulacastrum. Further research has to be done to isolate the pure bioactive compound that has high antioxidant potential.

Purification and Characterization of Bioactive Compounds Extracted from Suaeda Maritima Leaf and its Impact on Pathogenicity of Pseudomonas Aeruginosa in Catla Catla Fingerlings

“…Nayak et al (2018) reported presence of saponins, terpenoids, tannins, alkaloids and steroids in the methanol leaf extracts of Suaeda maritima, besides their presence in other mangroves such as Xylocarpus granatum, Avicennia marina, Salvador apersica and Avicennia o cinalis (Mulla and Chavan 2016). Similar to present study, ethanolic extract of Sesuviumportula castrum possesses phytochemicals like alkaloids, carbohydrates, cardiac glycosides, avonoids, phenols, saponins, sterols, terpenoids, quinones, diterpenes, and resins contributed to its therapeutic value (Chintalapani et al 2018).…”

Enhancement of in Vitro Antioxidant Potential of Terminalia Chebula by Various Fruit Extracts and Optimization of Concentration by Response Surface Methodology

“…The lack of fit F-value of 3.43 implies that the lack of fit is not significant relative to the pure error. This is in agreement with a study in which RSM was employed to optimize the conditions for antioxidant potential and polyphenols from apricot powder (Prunus armeniaca L.) using four independent variables: Methanol (20%, 35%, 50%, 65%, and 80%), solvent/sample ratio (10,15,20,25, and 30), temperature (20,30,40,50, and 60°C), and time (20,30,40,50, and 60 min). The results showed that antioxidant potential and total polyphenols in the experiments varied from 76.15% to 96.68% and 8.77 to 12.11 mg GAE/g, respectively.…”

“…It showed that combined formula antioxidant activity was better than single antioxidant and consistent with the regression model analysis [22]. In another study, the antioxidant activity of whole plant extracts of Sesuvium portulacastrum L. was checked by DPPH assay in which diethyl ether extract showed the highest total phenolic content and antioxidant potential among all the extracts of S. portulacastrum [15]. In the present study, the results of DPPH radical scavenging activity of ethanolic extract combination number 15 increased with increase in concentration representing a dose-dependent curve as shown in Figs scavenging activity on the DPPH radical which was found to be increasing with the increasing concentration and thus revealed dosedependent curves.…”

Comparative Study of Polyphenol, Flavonoid, and Antioxidant Activity of Various Medicinal Plants Collected From Different Altitudes

“…Reactive oxygen species (ROS) and nitrogen (RNS) species act as pro-oxidants that either induces oxidative stress or inhibit the antioxidant activity and cause damage to proteins, lipid peroxidation, and fragmentation of deoxyribonucleic acid strand [3]. The process ultimately leads to cellular injury, aging, cancer, and hepatic, neurodegenerative, cardiovascular, and renal disorders [4,5].…”