Phytochemical study of Gypsophila trichotoma wend. (Caryophyllaceae)

Krasteva, Ilina; Popov, I S; Balabanova, Vessela; Nikolov, S; Pencheva, Ivanka

doi:10.1590/s0100-40422008000500036

Cited by 16 publications

(8 citation statements)

References 11 publications

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“…The species is protected by the National Biodiversity Act. Our previous phytochemical studies of G. trichotoma showed the presence of triterpene saponins, flavonoids, sterols, and volatile compounds [6–8]. Saponarin, a naturally occurring apigenin-6-C-glucosyl-7-O-glucoside, is the main flavone glycoside isolated in a high amount (2 g) from G. trichotoma , for which preliminary in vitro [9] antioxidant activity was evaluated.…”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective and Antioxidant Effects of Saponarin, Isolated fromGypsophila trichotomaWend. on Paracetamol-Induced Liver Damage in Rats

Simeonova

Vitcheva

Kondeva-Burdina

et al. 2013

BioMed Research International

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The hepatoprotective potential of saponarin, isolated from Gypsophila trichotoma, was evaluated in vitro/in vivo using a hepatotoxicity model of paracetamol-induced liver injury. In freshly isolated rat hepatocytes, paracetamol (100 μmol) led to a significant decrease in cell viability, increased LDH leakage, decreased levels of cellular GSH, and elevated MDA quantity. Saponarin (60–0.006 μg/mL) preincubation, however, significantly ameliorated paracetamol-induced hepatotoxicity in a concentration-dependent manner. The beneficial effect of saponarin was also observed in vivo. Rats were challenged with paracetamol alone (600 mg/kg, i.p.) and after 7-day pretreatment with saponarin (80 mg/kg, oral gavage). Paracetamol toxicity was evidenced by increase in MDA quantity and decrease in cell GSH levels and antioxidant defence system. No changes in phase I enzyme activities of AH and EMND and cytochrome P 450 quantity were detected. Saponarin pretreatment resulted in significant increase in cell antioxidant defence system and GSH levels and decrease in lipid peroxidation. The biochemical changes are in good correlation with the histopathological data. Protective activity of saponarin was similar to the activity of positive control silymarin. On the basis of these results, it can be concluded that saponarin exerts antioxidant and hepatoprotective activity against paracetamol liver injury in vitro/in vivo.

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Section: Introductionmentioning

confidence: 99%

Hepatoprotective and Antioxidant Effects of Saponarin, Isolated fromGypsophila trichotomaWend. on Paracetamol-Induced Liver Damage in Rats

Simeonova

Vitcheva

Kondeva-Burdina

et al. 2013

BioMed Research International

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“…28 Stigmasta-4,22-diene-3,6-dione, an allelochemical substance, found also in Pistia stratiotes (Araceae) was established to inhibit the growth of some microalgae. 30 Previously, 5α-stigmast-7-en-3-one was reported only from three plant species: Gypsophila trichotoma (Caryophyllaceae), 31 Centratherum anthelminticum (Asteraceae) 32 and Coccinia indica (Cucurbitaceae), 33 while stigmasta--4,6-dien-3-one was only found to occur naturally in Senecio crassiflorus (Asteraceae) 34 and Prosopis alpataco (Fabaceae). 35 Several of the steroids listed in Table I are quite rare plant constituents.…”

Section: Resultsmentioning

confidence: 99%

Steroids from Poison Hemlock (Conium maculatum L.): A GC-MS analysis

Radulović

Đorđević

2011

JSCS

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The steroid content of Conium maculatum L. (Poison Hemlock), Apiaceae, a well-known weed plant species, was studied herein for the first time. This was achieved by detailed GC-MS analyses of twenty two samples (dichloromethane extracts of different plant organs of C. maculatum at three or four different stages of phenological development, collected from three locations). In total, twenty four different steroids were identified. Six steroids had an ergostane nucleus while the other ones possessed a stigmastane carbon framework. The identity of these compounds was determined by spectral means (MS fragmentation), GC co-injections with authentic standards and chemical transformation (silylation). Steroid compounds were noted to be the main chemical constituents of root extracts (up to 70 %) of this plant species in the last phase of development. The predominant ones were stigmasta-5,22- dien-3β-ol (stigmasterol) and stigmasta-5-en-3β-ol (β-sitosterol). In an attempt to classify the samples, principal component analysis (PCA) and agglomerative hierarchical clustering (AHC) were performed using steroid percentages as variables

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“…Previous researchers reported the isolation of six triterpene glucosides with gypsogenin as an aglycon from the plant [2 -6]. In a continuation of our phytochemical investigation on Gypsophila trichotoma [7], we describe the isolation and structure elucidation of three new sulfated oleanane-type saponins (1 -3).…”

Section: Introductionmentioning

confidence: 80%