Phytohemagglutinin: Inhibition of the Agglutinating Activity by N -Acetyl-D-Galactosamine

Abstract

The effect of simple sugars on the agglutinating activity of phytohemagglutinin was studied. N-Acetyl-d-galactosamine selectively inhibits the agglutination of leukocytes and erythro- cytes by phytohemagglutinin.

Isolation and Characterization of Factors in Sweet Potato Root Which Agglutinate Germinated Spores of Ceratocystis fimbriata, Black Rot Fungus

“…Also, much information has been accumulated on their nature and mode of action on animal cells (3,4,6,7,16,19). However, their role in the plant is unclear.…”

“…It has been shown that many plants contain phytohemagglutinins or lectins (3,4,6,18). Also, much information has been accumulated on their nature and mode of action on animal cells (3,4,6,7,16,19).…”

“…The factor showed differentimIl agglutinating activity toward the strains in the presence of unidentified low molecular weight factor(s) in the sweet potato root. These results support our earlier suggestion that the spore-agglutinating factors in host plants function as the determinants of specificity in some host-parasite interactions.It has been shown that many plants contain phytohemagglutinins or lectins (3,4,6,18). Also, much information has been accumulated on their nature and mode of action on animal cells (3,4,6,7,16,19).…”

Inhibition of broad spectrum hemagglutinin fromPseudomonas aeruginosa by D‐galactose and its derivatives

“…The BHA agglutinated also the erythrocytes from various animals, including rats, rabbits, dogs, mice and sheep. The titer of agglutination of papain or neuraminidase treated cells was always much higher than that obtained for untreated cells, resembling the behaviour of "incomplete" antibodies and certain phytohemagglutinins [ 1, 2,8,141 . These results are presented in table 1.…”

Mechanisms of the Adjuvant Effect of Nystatin on In Vitro Antibody Response of Mouse Spleen Cells

“…In addition to these B-cell mitogens, phytohemagglutinin (PHA) and concanavalin A (Con A), well-known mitogens for thymusdependent lymphocytes (T cells), exert adjuvanticity for antibody response to sheep 138 H. ISHIKAWA ET AL erythrocytes (SRBC) in mouse spleen cell cultures under appropriate conditions (8,32,33). Although receptors for PHA and Con A on T cells have been shown to be certain sugar moieties existing on the cell membrane (2,25,37), receptors for the B-cell mitogens on B cells are still obscure, and furthermore, the molecular mechanisms in triggering by these B-cell or T-cell mitogens of lymphocyte activation that leads to stimulation of DNA synthesis and to enhanced antibody response to antigenic stimulation are unknown.…”