Phytoremediation of 2,4‐dichlorophenol by <i>Brassica napus</i> hairy root cultures

Agostini, Elizabeth; Coniglio, María S.; Milrad, Silvia R.; Tigier, Horacio A.; Giulietti, Ana María

doi:10.1042/ba20020079

Cited by 108 publications

(57 citation statements)

References 24 publications

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“…Hairy root cultures of turnip (Brassica napus), are very efficient in degrading the toxic pesticide 2,4-dichlorophenol (2,4-DCP) (Agostini et al 2003). The resulting products have not yet been precisely identified both in terms of chemical nature and toxicity, but they are probably a mixture of polymers created by the action of peroxidases.…”

Section: Chemical Stressesmentioning

confidence: 99%

Peroxidases have more functions than a Swiss army knife

et al. 2005

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Plant peroxidases (class III peroxidases) are present in all land plants. They are members of a large multigenic family. Probably due to this high number of isoforms, and to a very heterogeneous regulation of their expression, plant peroxidases are involved in a broad range of physiological processes all along the plant life cycle. Due to two possible catalytic cycles, peroxidative and hydroxylic, peroxidases can generate reactive oxygen species (ROS) ( • OH, HOO• ), polymerise cell wall compounds, and regulate H 2 O 2 levels. By modulating their activity and expression following internal and external stimuli, peroxidases are prevalent at every stage of plant growth, including the demands that the plant meets in stressful conditions. These multifunctional enzymes can build a rigid wall or produce ROS to make it more flexible; they can prevent biological and chemical attacks by raising physical barriers or by counterattacking with a large production of ROS; they can be involved in a more peaceful symbiosis. They are finally present from the first hours of a plant's life until its last moments. Although some functions look paradoxical, the whole process is probably regulated by a fine-tuning that has yet to be elucidated. This review will discuss the factors that can influence this delicate balance.Keywords Evolution . ROS . (abiotic and biotic) stress . Cell wall loosening and cross-linking . Senescence . Fruit ripening . Symbiosis Multigenic family, evolution and homologyHeme peroxidases specific to plants belong to a superfamily that contains three different classes of peroxidases Communicated by P. Kumar

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Section: Chemical Stressesmentioning

confidence: 99%

Peroxidases have more functions than a Swiss army knife

et al. 2005

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“…Peroxidases have been implicated in a wide range of physiological processes such as auxin metabolism, lignin and suberin formation, cross-linking of cell wall components and defense against pathogens 19,20 . Peroxidases have also been implicated in the accumulation of heavy metals by some plant species and the degradation of toxic molecules and pesticides 21,22 . Polyphenol oxidases are oxidoreductases that catalyse oxidation of phenolic compounds and widely distributed with extracellular localization in plants.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Enzyme Expression in a Macrophytic Treated Crude Oil Soil Habitat: Implication for Enhanced Phytoremediation Potential Using Transgenic Botanicals

Edwin-Wosu¹,

Nkang²

2015

Insight Ecology

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Background: Several studies have demonstrated the success of phytoremediation and its advantages over conventional physical and biochemical (even engineered transgenics) methods, resulting in its use on many contaminated sites. A direct method for enhancing the effectiveness of phytoremediation is to over express the genes in transgenic plants that are involved in metabolism, uptake and/or transport of specific pollutants. Objective: A screen-house ecological study was carried out to evaluate the remediation potentials of three species of the Fabaceae Family (Peltophorum pterocarpum, Leucaena leucocephala and Crotolaria retusa) for cleaning up soils contaminated with crude oil petroleum hydrocarbons. Result: Enzymatic analysis and hydrocarbon index assessment of the species and the vegetated and non-vegetated soils showed that P. pterocarpum recorded significant (p<0.05) performance over C. retusa and L. leucocephala in the uptake of hydrocarbons (oil and Grease (OG), Total Hydrocarbon Content (THC), organic carbon and organic matter content]. Results showed significant (p<0.05) reduction in enzyme expression and soil hydrocarbon index. C. retusa recorded significant (p<0.05) increase over P. pterocarpum and L. leucocephala in enzyme expression and reduction in foliar hydrocarbon index, with significant (p<0.05) increase in soil hydrocarbon index. Conclusion: Crotolaria retusa can thus be recommended as an integral component of any bioremediation technology package for hydrocarbon polluted terrestrial environment and by it transgenic enhancement of indigenous enzymes.

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“…2,4-dichlopophenol (2,4-DCP), used in the manufacture of industrial and agricultural products like herbicides ( viz. 2,4-dichlorophenoxyacetic acid), pesticides, germicides, resins, antiseptics (Agostini et al 2003 ) is discharged as a contaminant in industrial effl uents. Long term exposure to this compound may permanently damage liver, skin, eyes, kidneys and are suspected to be carcinogens (Edwards and Santillo 1996 ) .…”

Section: Role Of Hairy Roots In Phytoremediationmentioning

confidence: 99%

Hairy Roots: A Promising Tool for Phytoremediation

Majumder

Jha

2011

Microorganisms in Environmental Management

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Environmental pollution caused by natural processes or anthropogenic activities is a major global problem. Although several physical and chemical strategies have been used for environmental remediation, these methods are expensive and associated with certain limitations. Phytoremediation is an alternative, biological approach where different plant species are used for the removal of pollutants from the environment or for converting toxic molecules to non toxic forms. Both organic and inorganic compounds of various types are the targets of phytoremediation. The technique is cheaper than other established methods and has several advantages like soil stabilization, production of biofuels, non invasiveness etc. Plants readily absorb certain compounds, otherwise considered contaminants, through the root system and utilize for their normal growth and development. Hairy roots of plants are among the several experimental systems which have been employed to improve the effi ciency of phytoremediation. Hairy root disease, characterized by a massive production of adventitious roots with numerous root hairs at the wound site, is caused by Agrobacterium rhizogenes , a gram negative soil bacterium. The bacterium transfers a segment (the transferred DNA or T-DNA) of its root inducing (Ri) megaplasmid into the plant genome, modifying the normal hormonal metabolism of the plant; this hormonal imbalance induces the formation of hairy roots at the site of infection. Apart from being suitable for the production of a wide array of valuable secondary metabolites, hairy roots possess tremendous potential for phytoremediation. Hairy roots of different plant species have been investigated for the accumulation and biodegradation of hazardous compounds. The present review summarizes the role of plant hairy roots in phytoremediation research.

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Phytoremediation of 2,4‐dichlorophenol by Brassica napus hairy root cultures

Cited by 108 publications

References 24 publications

Peroxidases have more functions than a Swiss army knife

Peroxidases have more functions than a Swiss army knife

Evaluation of Enzyme Expression in a Macrophytic Treated Crude Oil Soil Habitat: Implication for Enhanced Phytoremediation Potential Using Transgenic Botanicals

Hairy Roots: A Promising Tool for Phytoremediation

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