Phytosynthesized gold nanoparticles from C. roxburghii DC. leaf and their toxic effects on normal and cancer cell lines

Balashanmugam, Pannerselvam; Prabhu, D.; Balakumaran, Manickam Dakshinamoorthi; Kalaichelvan, P. T.

doi:10.1016/j.jphotobiol.2016.10.013

Cited by 67 publications

(41 citation statements)

References 48 publications

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“…Synthesis of Size-controlled nanoparticles for stable systems have been prepared through combined action of solvated metal atom dispersion technique [42]. The size and morphology of prepared silver nanoparticles were analyzed using transmission electron microscopy (TEM) ( Figure 3), which clearly exhibited the formation of polydisperse spherical and different magnitudes of crystalline AgNPs ranging from 20 to 50 nm in diameter, consistent with results of previous studies [43,44]. Elementary compositions of these biosynthesized silver nanoparticles were determined by energy-dispersive X-ray (EDX) spectrum analysis ( Figure 4).…”

Section: Biological Synthesis and Silver Nanoparticles Characterizationsupporting

confidence: 83%

Facile Synthesis of Silver Nanoparticles Using Asian Spider Flower and Its In Vitro Cytotoxic Activity Against Human Breast Carcinoma Cells

Balashanmugam

Prabhu²,

Thiyagarajan

et al. 2020

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Cancer is one of the most dangerous threats to human health and possibly the utmost task for current medicine. Currently, bio-based synthesis of nanoparticles from plants has gained much interest due to its potential medicinal applications. In the present study, a biological approach was employed for biogenic (green) synthesis of silver nanoparticles (AgNPs) using dried leaf extract of Asian spider flower (Asf). The biogenic synthesis of Asf-AgNPs (Asian spider flower-Silver nanoparticles) was established using ultra violet-visible (UV-vis) spectra which exhibited a wide superficial plasmon resonance of AgNPs at 445 nm. These nanoparticles clearly showed the formation of poly-disperse crystalline solids (spherical shape) with particle size range of <50 nm based on observation under a transmission electron microscope (TEM). Infrared spectroscopy (FTIR) revealed carboxylic acids (C = O stretch) known to act as a capping agent and a reductant in plant extracts. Elemental silver signal peak was observed in the graph obtained from energy-dispersive X-ray (EDX) analysis. Biocompatibility tests for Asf-AgNPs at different doses were evaluated against human breast cancer cells (MCF7) for cell viability and apoptotic analysis. According to the evaluation, biosynthesized Asf-AgNPs could prevent the explosion of human breast tumor cells (MCF7) in IC50 at a dose of 40 μg/mL after 48 h of treatment. The results obtained in the IC50 dosage treatments were statistically significant (p < 0.05) when compared with control. Nuclear damage of cells was further investigated using annexin V-FITC/PI dual staining and DAPI (4′,6-diamidino-2-phenylindole) staining method. Bright blue fluorescence with condensed and fragmented chromatin was observed. Western blot analysis showed increased expression levels of caspases-3 and 9 (apoptotic proteins). These results indicate that bio-approached AgNPs synthesized through Asf plant extract could be used as potential therapeutic medications for human cancer cells.

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Section: Biological Synthesis and Silver Nanoparticles Characterizationsupporting

confidence: 83%

Facile Synthesis of Silver Nanoparticles Using Asian Spider Flower and Its In Vitro Cytotoxic Activity Against Human Breast Carcinoma Cells

Balashanmugam

Prabhu²,

Thiyagarajan

et al. 2020

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“…Moreover it eradicates the complicated procedure of preserving microbial cultures and could also be stretched to bulk-scale production. Previous literature revealed that terrestrial plants have been extensively utilized for the preparation of metal nanoparticles [7][8][9][10][11][12] and only a limited number of reports on nanogold synthesis using renewable marine resources like marine macroalgae (seaweeds) [13,14]. Due to the richness of bioactive metabolites, marine macroalgae are promising source for both industrial and biotechnological applications.…”

Section: Introductionmentioning

confidence: 99%

Optimization of physicochemical parameters in the biofabrication of gold nanoparticles using marine macroalgae Padina tetrastromatica and its catalytic efficacy in the degradation of organic dyes

Princy¹,

Gopinath

2018

J Nanostruct Chem

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Green synthesis of metallic nanoparticles by means of renewable bioresources has emerged as a new trend in current nanotechnology research with improved environmental safety. In the current study, monodispersed gold nanoparticles (AuNPs) with excellent stability were prepared in a completely green and cost effective manner using aqueous extract of marine macroalgae-Padina tetrastromatica. The influence of reaction conditions such as the quantity of seaweed extract, temperature, precursor metal ion concentration, reaction time and pH on the biosynthesis of nanoparticle was evaluated spectroscopically and also with the help of high resolution transmission electron microscopy (HR-TEM). These physicochemical parameters not only affected the rate of formation but also the size and morphology of resultant nanoparticles. Optimum conditions resulted in the generation of nearly spherical AuNPs having an average particle size of 11.4 nm. The high crystallinity of the biogenic AuNPs was confirmed from characteristic diffraction peaks in XRD profile, clear lattice fringes in the HR-TEM image and bright circular spots in the SAED pattern. The presence of metallic gold was evidenced from EDAX profile. FTIR study revealed the role of secondary metabolites in the bioreduction as well as stabilization of AuNPs. The study also highlights the spectroscopic investigation on the catalytic efficacy of the biosynthesized AuNPs in the reduction reactions of hazardous organic dyes, eosin yellow and Congo red using sodium borohydride, which have a pseudo-first order kinetics. Thus, the biosynthesized metal nanoparticles using renewable marine resources like seaweeds act as promising materials for the application in environmental protection.

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“…The biogenic synthesis of AuNPs was carried out according to Balashanmugam et al [13] method with modifications. Briefly, 1.0 ml of the aqueous leaf extract was added to 9.0 ml of 1.0 mmol of HAuCl4 separately and incubated in the dark at room temperature for 24 h. The visual observation of change in color from golden yellow to ruby red color indicates the formation of AuNPs.…”

Section: Biogenic Synthesis Of Gold Nanoparticlesmentioning

confidence: 99%

“…The analysis was carried out with Cu Kα radiation at a wavelength of 1.5406 Å with the operating condition at 40 kV, 30 mA. The XRD pattern was scanned in the 2θ from 30 ° to 70 ° with a step size of 0.04 ° per second [13]. The PA-1 ovarian teratocarcinoma cell line and A549 lung adenocarcinoma cell line was obtained from the national center for cell sciences (NCCS), Pune, India.…”

Section: X-ray Diffraction (Xrd)mentioning

confidence: 99%

In Vitro Cytotoxicity and Antioxidant Evaluation of Biogenic Synthesized Gold Nanoparticles From Marsilea Quadrifolia on Lung and Ovarian Cancer Cells

Balashanmugam

Christas²,

Kowsalya³

2018

Int J App Pharm

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Objective: The biogenic gold nanoparticles are considered to be extremely impressive for its wide range of applications in pharmaceutics and therapeutics. The present study was aimed at the biogenic synthesis of gold nanoparticles (AuNPs) from Marsilea quadrifolia aqueous extract and to investigate its antioxidant property and cytotoxic effect on human ovarian teratocarcinoma (PA-1) and lung adenocarcinoma (A549) cell lines.Methods: The biogenic AuNPs was synthesized using an aqueous extract of Marsilea quadrifolia. The synthesized biogenic AuNPs were characterized by ultraviolet (UV) visible spectroscopy, transmission electron microscopy (TEM), energy dispersive X-ray analysis (EDX) and X-ray diffraction (XRD). The biogenic AuNPs was assessed for its stability over a period of time and antioxidant activity. The cytotoxicity of biogenic AuNPs against PA-1 and A549 cell lines was studied using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.Results: The synthesized biogenic AuNPs showed peculiar ruby red color and a surface plasmon resonance (SPR) peak at 544 nm in the UV-Vis spectrum. The characterization of biogenic AuNPs by TEM, EDX and XRD revealed well dispersed spherical particles ranging from 10-40 nm and the presence of elemental gold and its crystalline nature, respectively. The AuNPs showed good stability and the scavenging activity at 50 μg/ml. The in vitro cytotoxicity of biogenic AuNPs against PA-1 and A549 cell lines recorded half maximal inhibitory concentration (IC50) of 45.88 μg/ml and 52.015 μg/ml, respectively.Conclusion: The biogenic AuNPs demonstrated superior antioxidant and antiproliferative activities against cancer cell lines.

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Phytosynthesized gold nanoparticles from C. roxburghii DC. leaf and their toxic effects on normal and cancer cell lines

Cited by 67 publications

References 48 publications

Facile Synthesis of Silver Nanoparticles Using Asian Spider Flower and Its In Vitro Cytotoxic Activity Against Human Breast Carcinoma Cells

Facile Synthesis of Silver Nanoparticles Using Asian Spider Flower and Its In Vitro Cytotoxic Activity Against Human Breast Carcinoma Cells

Optimization of physicochemical parameters in the biofabrication of gold nanoparticles using marine macroalgae Padina tetrastromatica and its catalytic efficacy in the degradation of organic dyes

In Vitro Cytotoxicity and Antioxidant Evaluation of Biogenic Synthesized Gold Nanoparticles From Marsilea Quadrifolia on Lung and Ovarian Cancer Cells

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