Picea wilsonii transcription factor NAC2 enhanced plant tolerance to abiotic stress and participated in RFCP1-regulated flowering time

Zhang, Hehua; Cui, Xiaoyue; Guo, Yuxiao; Luo, Chaobing; Zhang, Lingyun

doi:10.1007/s11103-018-0792-z

Cited by 29 publications

(20 citation statements)

References 86 publications

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“…Picea wilsonii, an endemic species of coniferous forests, which is best characterized by tolerance to abiotic stress and environment adaptability, is widespread in northern China. Picea wilsonii also has ornamental value in landscaping based on its impressing appearance and lush green crown [28]. Given its advantageous characteristics, it is of great significance to screen and reveal the molecular mechanisms of its abiotic resistance.…”

Section: Introductionmentioning

confidence: 99%

NAC Transcription Factor PwNAC11 Activates ERD1 by Interaction with ABF3 and DREB2A to Enhance Drought Tolerance in Transgenic Arabidopsis

Liu

et al. 2021

IJMS

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NAC (NAM, ATAF1/2, and CUC2) transcription factors are ubiquitously distributed in eukaryotes and play significant roles in stress response. However, the functional verifications of NACs in Picea (P.) wilsonii remain largely uncharacterized. Here, we identified the NAC transcription factor PwNAC11 as a mediator of drought stress, which was significantly upregulated in P. wilsonii under drought and abscisic acid (ABA) treatments. Yeast two-hybrid assays showed that both the full length and C-terminal of PwNAC11 had transcriptional activation activity and PwNAC11 protein cannot form a homodimer by itself. Subcellular observation demonstrated that PwNAC11 protein was located in nucleus. The overexpression of PwNAC11 in Arabidopsis obviously improved the tolerance to drought stress but delayed flowering time under nonstress conditions. The steady-state level of antioxidant enzymes’ activities and light energy conversion efficiency were significantly increased in PwNAC11 transgenic lines under dehydration compared to wild plants. PwNAC11 transgenic lines showed hypersensitivity to ABA and PwNAC11 activated the expression of the downstream gene ERD1 by binding to ABA-responsive elements (ABREs) instead of drought-responsive elements (DREs). Genetic evidence demonstrated that PwNAC11 physically interacted with an ABA-induced protein—ABRE Binding Factor3 (ABF3)—and promoted the activation of ERD1 promoter, which implied an ABA-dependent signaling cascade controlled by PwNAC11. In addition, qRT-PCR and yeast assays showed that an ABA-independent gene—DREB2A—was also probably involved in PwNAC11-mediated drought stress response. Taken together, our results provide the evidence that PwNAC11 plays a dominant role in plants positively responding to early drought stress and ABF3 and DREB2A synergistically regulate the expression of ERD1.

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Section: Introductionmentioning

confidence: 99%

NAC Transcription Factor PwNAC11 Activates ERD1 by Interaction with ABF3 and DREB2A to Enhance Drought Tolerance in Transgenic Arabidopsis

Liu

et al. 2021

IJMS

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“…The highly diverse transcription regulatory region at the C-terminal of NAC proteins confers transcriptional activation diversity of different NAC TFs [11,12]. NAC TFs have been reported to participate in various biological processes in plants, such as formation of second cell wall, programmed cell death and flowering period of plant [13][14][15]. In addition, accumulating evidence has indicated that NAC TFs widely play crucial roles in plants' response to abiotic stresses, such as drought, salinity, and cold stress.…”

Section: Introductionmentioning

confidence: 99%

Identification and Expression of NAC Transcription Factors of Vaccinium corymbosum L. in Response to Drought Stress

Liang

Wang

Sun

et al. 2019

Forests

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Research Highlights: Phenotypic changes and expression profiles, phylogeny, conserved motifs, and expression correlations of NAC (NAM, ATAF1, ATAF2 and CUC2) transcription factors (TFs) in blueberry genome were detected under drought stress, and the expression patterns and functions of 12 NACs were analyzed. Background and Objectives: Blueberry is an important shrub species with a high level of flavonoids in fruit, which are implicated in a broad range of health benefits. However, the molecular mechanism of this shrub species in response to drought stress still remains elusive. NAC TFs widely participate in stress tolerance in many plant species. The characterization and expression profiles of NAC TFs were analyzed on the basis of genome data in blueberry when subjected to drought stress. Materials and Methods: Combined with the analysis of chlorophyll a fluorescence and endogenous phytohormones, the phenotypic changes of blueberry under drought stress were observed. The phylogenetic tree, conserved motifs, differently expressed genes, and expression correlation were determined by means of multiple bioinformatics analysis. The expression profiles of NACs in different organs were examined and compared through RNA-seq and qRT-PCR assay. Results: The chlorophyll a fluorescence parameters φPo, φEo, φRo, and PIabs of leaves were significantly inhibited under drought stress. ABA (abscisic acid) content noticeably increased over the duration of drought, whereas GA3 (gibberellic acid) and IAA (indole acetic acid) content decreased continuously. A total of 158 NACs were identified in blueberry genome and 62 NACs were differently expressed in leaf and root of blueberry under drought stress. Among them, 14 NACs were significantly correlated with the expression of other NAC genes. Conclusions: Our results revealed the phenotypic changes of this shrub under drought stress and linked them with NAC TFs, which are potentially involved in the process of response to drought stress.

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“…Eight-week-old seedlings of P. wilsonii were used for transcriptome sequencing. Seeds were germinated in the greenhouse, and seedlings were grown at 19 C and 16 h light/8 h dark photoperiod [20]. To carry out the salt treatment, seedlings were grown on Murashige and Skoog (MS) liquid medium supplemented with 300 mM NaCl for 6 h [20,21].…”

Section: Plant Materials and Treatmentsmentioning

confidence: 99%

“…Seeds were germinated in the greenhouse, and seedlings were grown at 19 C and 16 h light/8 h dark photoperiod [20]. To carry out the salt treatment, seedlings were grown on Murashige and Skoog (MS) liquid medium supplemented with 300 mM NaCl for 6 h [20,21]. To conduct the drought treatment, seedlings were placed on dry filter paper with 35% relative humidity at 20 C for 6 h [22,23].…”

Section: Plant Materials and Treatmentsmentioning

confidence: 99%

“…Additionally, qRT-PCR experiments were performed using 8-week-old P. wilsonii seedlings treated with various abiotic stresses. Briefly, seedlings grown on solid matrices were supplied with water (control) or water containing 300 mM NaCl, 100 lM ABA, 20 mM H 2 O 2 , 100 lM gibberellin (GA 3 ), 100 lM indole-3-acetic acid (IAA), 100 lM methyljasmonate (Me-JA), 200 lM salicylic acid (SA) and ethylene (ET) released from 5 mM ethephon (ETH) solution for 0, 3, 6 and 12 h [20,[24][25][26]. To perform the drought treatment, watering was withheld for 0, 7, 14 and 21 days.…”

Section: Plant Materials and Treatmentsmentioning

confidence: 99%

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Identification and characterization ofNACgenes in response to abiotic stress conditions inPicea wilsoniiusing transcriptome sequencing

Guo

Zhang

Yuan

et al. 2020

Biotechnology & Biotechnological Equipment

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Picea wilsonii is widely used for a forestation and landscaping in China. However, the molecular mechanisms underlying abiotic stress tolerance in this species remain elusive. In this study, we examined the transcriptomic changes induced by drought and salt stress in 8-week-old seedlings of P. wilsonii via RNA-Seq analysis; seedlings grown under normal conditions (without abiotic stress) served as a control. Approximately 36.26 Gb of transcriptome data were obtained from the three treatments. A total of 4171 (2579 up-regulated and 1732 down-regulated) and 3438 (1592 up-regulated and 1706 down-regulated) differentially expressed genes were identified under drought and salt treatments, respectively. Both RNA-Seq and qRT-PCR analyses showed that the expression of PwNAC genes, including PwNAC11, 24, 36, 38, 45, 47, 51 and 52, was greatly induced not only by salt stress but also by drought treatment, suggesting that these genes are involved in both salt and drought stress resistance in P. wilsonii. This is the first report of the transcriptome analysis of P. wilsonii. Our data will serve as a valuable genomic resource for the further characterization of the molecular mechanisms underlying abiotic stress tolerance in P. wilsonii.

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Picea wilsonii transcription factor NAC2 enhanced plant tolerance to abiotic stress and participated in RFCP1-regulated flowering time

Cited by 29 publications

References 86 publications

NAC Transcription Factor PwNAC11 Activates ERD1 by Interaction with ABF3 and DREB2A to Enhance Drought Tolerance in Transgenic Arabidopsis

NAC Transcription Factor PwNAC11 Activates ERD1 by Interaction with ABF3 and DREB2A to Enhance Drought Tolerance in Transgenic Arabidopsis

Identification and Expression of NAC Transcription Factors of Vaccinium corymbosum L. in Response to Drought Stress

Identification and characterization ofNACgenes in response to abiotic stress conditions inPicea wilsoniiusing transcriptome sequencing

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