Piezoelectric immunochip for the detection of dengue fever in viremia phase

Wu, Tzong-Zeng; Su, Chih-Cheng; Chen, Li‐Kuang; Yang, Huihua; Tai, Dar-Fu; Peng, Kou-Cheng

doi:10.1016/j.bios.2004.12.019

Cited by 71 publications

(27 citation statements)

References 18 publications

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“…The 15-mer peptide (template) combined with functional monomers in solution and became spatially fixed in a solid polymer film with a thickness of ϳ70 nm after UV irradiation (22 ). Subsequently, the resulting chip was treated with alkaline, neutral, or acidic solutions to remove the template and to effectively clean the chip surface.…”

Section: Resultsmentioning

confidence: 99%

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Artificial Receptors in Serologic Tests for the Early Diagnosis of Dengue Virus Infection

Tai

Lin

et al. 2006

Clinical Chemistry

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Background: Because of the range and nonspecificity of clinical presentations of dengue virus infections, we felt there was a need to create diagnostic tests. We used artificial receptors for the virus to develop serologic assays to detect dengue virus infection. Methods: We coated a quartz crystal microbalance (QCM) with molecularly imprinted polymers specific for nonstructural protein 1 of flavivirus. These artificial receptors were specifically created on a QCM chip by polymerization of monomers and were cross-linked in the presence of the epitope site of nonstructural protein 1. We tested serum samples from patients with confirmed cases of dengue reported to the Center for Disease Control in Taipei. Samples were diluted 100-fold; no other sample pretreatment was used. The QCM response was compared with results of monoclonal ELISA. Results: QCM signals were >15 Hz in 18 of 21 (86%) of dengue samples and in 0 of 16 control samples. The correlation (r 2 ) of the QCM response and the ELISA result was 0.73. Within-run and run-to-run imprecisions (CV) were 4%-28% and 10%-32%, respectively. Conclusions: The described assay offers a serologic technique for diagnosis of early viremia. The results illustrate the potential of well-organized polymers on the highly sensitive sensor system for diagnostic and biotechnological applications.

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Section: Resultsmentioning

confidence: 99%

“…NS1 antigen ELISA was performed with mAb 8-1, and the cutoff value is 0.35A. Antibody-QCM tests were performed previously (22). MIP-QCM tests were performed after the pretreatment with Cibacrown gel.…”

Section: Formation and Application Of The Rigid Mip-qcm Assay Withoutmentioning

confidence: 99%

Artificial Receptors in Serologic Tests for the Early Diagnosis of Dengue Virus Infection

Tai

Lin

et al. 2006

Clinical Chemistry

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“…Piezoelectric transduction enables label-free detection of biorecognition events and has been used in microgravimetric devices, generally known as quartz-crystal microbalance (QCM), for different applications (Yang et al, 1998;Etchenique and Brudny, 2000;Liu et al, 2004;Su and Li, 2005;Wu et al, 2005;Modin et al, 2006;Encarnação et al, 2007a;Ferreira et al, 2007;Mitomo et al, 2007). These sensors are driven to mechanically resonate at a particular frequency ( f 0 ) (Kö blinger et al, 1995;Laricchia-Robbio and Revoltella, 2004), that is dependent on the deposition of mass according to the Sauerbrey equation (Sauerbrey, 1959) (Equation (1)).…”

Section: Introductionmentioning

confidence: 99%

Piezoelectric biosensors assisted with electroacoustic impedance spectroscopy: a tool for accurate quantitative molecular recognition analysis

Encarnação

Baltazar

Stallinga

et al. 2008

J of Molecular Recognition

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In this work, electroacoustic impedance analysis based on a modified Butterworth-Van Dyke (BVD) model is used to complement resonance frequency measurements of piezoelectric crystal sensors for the identification and removal of interfering signals. This approach enables the accurate use of the Sauerbrey correlation to establish a direct relationship between mass deposited at the sensor surface and measured frequency variations. Kinetic models can thus be evaluated and binding constants estimated directly from the measured data. We further demonstrate the usefulness of this approach by applying it to the study of the formation of 11-hydroxy-1-undecanothiol self-assembled monolayers (SAM) as well as to the binding of streptavidin to immobilized biotin. Kinetic and equilibrium parameters were estimated from transient analysis, adsorption isotherms, Scatchard and Hill plots obtained from the frequency data for both the alkanethiol and streptavidin films. This strategy based on electroacoustic impedance assisted quartz-crystal microbalance (QCM) biosensors is expected to be a major contribution for the use of these piezoelectric devices as a reliable and cheap detection system that can easily be integrated into analytical techniques.

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“…The addition of a protein A layer to orientate immobilized antibodies further enhanced sensitivity. 83 Sample pretreatment was optimized, and spiked samples and clinical specimens including five dengue virus serotype 2 (DENV2)-positive and ten dengue-negative sera were analyzed. The resulting immunochips could distinguish DENV2-positive from dengue-negative samples.…”

Section: Quartz Crystal Microbalance (Qcm)mentioning

confidence: 99%

Diagnosing dengue virus infection: rapid tests and the role of micro/nanotechnologies

Zhang

Salieb-Beugelaar

Nigo

et al. 2015

Nanomedicine: Nanotechnology, Biology and Medicine

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Piezoelectric immunochip for the detection of dengue fever in viremia phase

Cited by 71 publications

References 18 publications

Artificial Receptors in Serologic Tests for the Early Diagnosis of Dengue Virus Infection

Artificial Receptors in Serologic Tests for the Early Diagnosis of Dengue Virus Infection

Piezoelectric biosensors assisted with electroacoustic impedance spectroscopy: a tool for accurate quantitative molecular recognition analysis

Diagnosing dengue virus infection: rapid tests and the role of micro/nanotechnologies

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