Pigment composition, spectral characterization and photosynthetic parameters in Chryso-chromulma polylepis

Johnsen, Geir; Sakshaug, Egil; Vernet, María

doi:10.3354/meps083241

Cited by 40 publications

(37 citation statements)

References 26 publications

(31 reference statements)

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“…Nevertheless, there is still no concrete evidence for this hypothesis. In fact, at least 2 research groups (Haxo 1985, Johnsen et al 1992, Johnsen & Sakshaug 1993) have shown a light-harvesting function for 19'-hexanoyloxyfucoxanthin. Recently, Stolte et al (2000) have proposed that the pathway of synthesis of carotenoids in Emiliania huxleyi (a haptophyte) has steps leading from diadinoxanthin to fucoxanthin and from fucoxanthin to 19'-hexanoyloxyfucoxanthin.…”

Section: Photoadaptation and Photoacclimation Of Phytoplankton Populamentioning

confidence: 99%

Variability in pigment composition and optical characteristics of phytoplankton in the Labrador Sea and the Central North Atlantic

Lutz¹,

Sathyendranath²,

Head³

et al. 2003

Mar. Ecol. Prog. Ser.

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The relationships between photoadaptation, photoacclimation, cell size and the optical characteristics of the phytoplankton community were studied in 2 areas of the Atlantic Ocean. Pigment composition, absorption and fluorescence excitation spectra were analyzed for samples collected during 2 spring cruises: one in the Labrador Sea and the other in the Central North Atlantic. Photoadaptation (i.e. evolutionary adaptation of different species leading to acquisition of different pigment composition) was evident in the distribution of the main phytoplankton pigments in the area. Photoacclimation (i.e. temporary changes in pigment concentrations in a given species) was also noticeable in the changes in pigment composition and optical characteristics of phytoplankton with changes in depth. Size fractionation of samples from the depth of the chlorophyll a (chl a) maximum showed that, on average, chl a concentration and the values of absorption and fluorescence were dominated by the > 2 µm fraction of phytoplankton. Spectral variations in absorption and fluorescence excitation were, however, similar for the small and for the large size fractions. A distinct regional distribution of algal groups was observed, in which prokaryotic picophytoplankton (size class < 2 µm) dominated in oligotrophic subtropical regions and larger cells (size class > 2 µm) dominated in coastal areas and at higher latitudes. Some significant relationships were observed between the relative abundances of pigments characteristic of certain algal groups and optical properties of the sample. For example, the ratio of absorption at 440 nm to that at 676 nm was positively correlated with the ratio of zeaxanthin to chl a, indicating high abundance of picophytoplankton, especially the cyanobacteria Synechococcus and Prochlorococcus. The ratio of absorption at 555 nm to that at 623 nm was positively correlated with the abundance of phycoerythrin-containing Synechococcus. These results show that useful information about phytoplankton group composition and photoacclimation state can be retrieved from optical properties at a regional scale.

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Section: Photoadaptation and Photoacclimation Of Phytoplankton Populamentioning

confidence: 99%

Variability in pigment composition and optical characteristics of phytoplankton in the Labrador Sea and the Central North Atlantic

Lutz¹,

Sathyendranath²,

Head³

et al. 2003

Mar. Ecol. Prog. Ser.

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“…Chl c represents a mixture of slightly spectrally distinct components: chl c,, chl C?, chl c3 and magnesium 2,4-divinyl phaeoporphyrin a, monomethyl ester (Mg-D). These have in vivo absorption maxima at approximately 460 to 470, 586 and 635 nm (Rowan 1989, Bidigare et al 1990a, Johnsen et al 1992, Johnsen & Sakshaug 1993.…”

Section: Introductionmentioning

confidence: 99%

“…The major light-harvesting carotenoids, i.e. the fucoxanthins and the 19'-acyloxyfucoxanthins together with pendinin and prasinoxanthin absorb in vivo mainly at 450 to 550 nm (Prezelin & Boczar 1986, Bidigare et al 1987, 1990b, Sathyendranath et al 1987, Johnsen et al 1992). Phycobiliprotein-dominated pigment systems of many cyanobacteria and cryptophytes absorb green to orange light (480 to 600 nm) efficiently (Prezelin & Boczar 1986, Bidigare et al 1987.…”

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

“…Chl a concentration and purity were estimated spectrophotometrically together with HPLC (high-performance liquid chromatography) for normalizing of in vivo absorption spectra to chl a (Jeffrey & Humprey 1975, Johnsen et al 1992. Pigment analyses were carried out with a Merck & Hitachi L-6200 HPLC equipped with a SPHERI-5 RP-18 reverse-phase C-18 column (Brownlee Labs 25 cm X 4.6 mm, 5 vm particles; Johnsen et al 1992). Extracts of 50 to 100 p1 were injected into the HPLC and detection performed at 440 nm in a Hitachi Spectrophotometer Model U-2000 fitted with a flow-through cell using the solvent system presented by Mantoura & Llewellyn (1983).…”

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confidence: 99%

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In vivo absorption characteristics in 10 classes of bloom-forming phytoplankton: taxonomic characteristics and responses to photoadaptation by means of discriminant and HPLC analysis

Johnsen¹,

Samset²,

Granskog³

et al. 1994

Mar. Ecol. Prog. Ser.

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134

107

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The spectral light absorption characteristics (400 to 700 nm) of 10 main classes, covering 31 species, of bloom-forming phytoplankton (diatoms, dinoflagellates, prymnesiophytes, euglenophytes, prasinophytes, raphidophytes, cryptophytes, chlorophytes, chrysophytes and cyanobacteria) have been examined. The survey is based on in vivo chlorophyll (chl) a-specific light absorption spectra ["a&), 400 to 700 nm] of low-and high-light adapted monocultures grown in the laboratory. Pigments were isolated by means of high-performance liquid chromatography (HPLC) to obtain visible spectra of isolated pigments to identify peaks and shoulders of the in vivo absorption spectra. A total of 217 "a&) spectra were log-transformed and normalized at 675 nm [a,,,(h)] to minimize photoadaptational effects on the spectral characteristics due to differences in pigment composition and the package effect. These alog(h) spectra were analyzed by stepwise discriminant analysis to determine sets of optimum wavelengths for classification. Discrimination and classification were most effective when lowand high-light adapted phytoplankton were grouped separately. A set of only 3 wavelengths (481,535, 649 nm) chosen on the basis of discriminant analysis classified, according to the jackknife technique, 93% of the a,,,(h) spectra. By using combinations of 4 (481, 535, 586, 649 nm) or 5 (481, 535, 586, 628, 649 nm) chosen wavelengths, 97 to 99% of the spectra were classified correctly. For pooled data (lowand high-light adapted cells), 60 to 86% of the spectra were correctly identified using a combination of 3 to 5 selected wavelengths, indicating that variations due to photoadaptation were not entirely removed by log-transforming and scaling of the spectra at 675 nm. By using the above combination of 3 wavelengths, 4 main groups of phytoplankton were clearly separated, depending mainly on their accessory chlorophylls, i.e. chl b (prasinophytes, euglenophytes, chlorophytes), chl c , and/or c;?(diatoms, dinoflagellates, prymnesiophytes, chrysophytes, raphidophytes, cryptophytes), chl c3 (toxic prymnesiophytes and dinoflagellates) and no accessory chlorophylls (cyanobacteria). The wavelengths employed here correspond to the peaks and shoulders of the in vivo absorption spectra. We conclude that different phytoplankton classes may be identified during blooms on the basis of in situ bio-optical measurements at 3 to 5 appropriately chosen wavelengths.

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Growth, Chl a content, photosynthesis, and elemental composition in polar and temperate microalgae

Lacour

Larivière

Babin

2016

Limnology & Oceanography

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Polar microalgae live under extreme environmental conditions: permanently low temperatures (−1.7°C to +5°C) and extreme variations in irradiance and day length. These conditions may have led to various specific adaptations allowing Arctic phytoplankton to become specialists under these conditions. The goal of this study is to derive, for polar microalgae, empirical relationships between key physiological parameters (growth rate, photosynthesis–irradiance curve parameters, Chl a : C, and N : C ratios) and growth temperature and irradiance in nutrient replete cultures. Ecophysiological characteristics of polar and temperate microalgae were also compared in order to highlight some strategies that are specific to the polar environment. Most of the polar species are psychrophilic. Polar microalgae have low light‐saturated growth rates (μm) and very low light saturation parameters for growth (KE) but similar initial slopes of their growth–irradiance curve (αµ = μm/KE). Low temperatures probably account for low μm and KE in polar species. The C : Chl a ratios (θ) of polar species are similar to those of temperate species although they have much lower growth rates, which implies major differences in energy allocation. Polar microalgae also exhibit very unique photosynthetic properties [low light saturation parameters for photosynthesis (EK), low maximum photosynthetic rates ( PmC), decreasing PmC and decreasing initial slope of the photosynthesis vs. irradiance curve (α*) with increasing irradiance] and have lower C : N ratios than their temperate cousins, which may be related to a much higher protein content. Some of the implications of these findings in terms of adaptation/acclimation to the environment in which polar species evolve are discussed.

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Pigment composition, spectral characterization and photosynthetic parameters in Chryso-chromulma polylepis

Cited by 40 publications

References 26 publications

Variability in pigment composition and optical characteristics of phytoplankton in the Labrador Sea and the Central North Atlantic

Variability in pigment composition and optical characteristics of phytoplankton in the Labrador Sea and the Central North Atlantic

In vivo absorption characteristics in 10 classes of bloom-forming phytoplankton: taxonomic characteristics and responses to photoadaptation by means of discriminant and HPLC analysis

Growth, Chl a content, photosynthesis, and elemental composition in polar and temperate microalgae

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