PIL5 represses floral transition in Arabidopsis under long day conditions

Wu, Minjie; Liu, Dongdong; Wakeel, Abdul; Upreti, Sakila; Liu, Yihua; Song, Ge; Wu, Junyu; Liu, Bohan; Gan, Yinbo

doi:10.1016/j.bbrc.2018.03.179

Cited by 11 publications

(7 citation statements)

References 44 publications

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“…2D). Even though the differences between PIF1a-defective and WT lines are relatively small and are only observed when counting the number of leaves from germination to anthesis (but not when counting the number of days), they are statistically significant and consistent with the observation that PIF1 regulates flowering time in Arabidopsis (Wu et al, 2018) (Fig. 5).…”

Section: Pif1a Is the Main Pif1 Homolog Regulating Many Other Processes Including Some That Are Not Regulated By Pifs In Arabidopsissupporting

confidence: 81%

“…Arabidopsis pif1 mutants show early flowering and up-regulated expression of the major flowering-promoting genes (Wu et al, 2018). We assessed the flowering time of tomato pif1a and pif1b mutants by measuring two different parameters: (1) the number of leaves produced when the first flower reached anthesis and (2) the number of days from sowing to anthesis.…”

Section: Loss Of Pif1a Might Results In Early Floweringmentioning

confidence: 99%

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Conserved and newly acquired roles of PIF1 homologs in tomato (Solanum lycopersicum)

Simón-Moya

Barja

Morelli

et al. 2021

Preprint

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PHYTOCHROME INTERACTING FACTORS (PIFs) are transcription factors that interact with the photoreceptors phytochromes and integrate multiple signaling pathways related to light, temperature, defense and hormone responses. PIFs have been extensively studied in Arabidopsis thaliana, but less is known about their roles in other species. Here, we investigate the role of the two homologs of PIF1 found in tomato (Solanum lycopersicum), namely PIF1a and PIF1b. Analysis of gene expression showed very different patterns, indicating a potential evolutionary divergence in their roles. At the protein level, light regulated the stability of PIF1a, but not PIF1b, further supporting a functional divergence. Phenotypic analyses of CRISPR-Cas9-generated tomato mutants defective in PIF1a or PIF1b or both revealed conserved and newly acquired roles compared to Arabidopsis PIF1. Both PIF1a or PIF1b were found to regulate seed germination, photosynthetic pigment biosynthesis and fruit production. However, only PIF1a-defective mutants showed defects on root hair elongation, flowering time and fruit growth and softening. We did not identify any process altered only in plants lacking PIF1b. Together, these data show that neofunctionalization has taken place in tomato, illustrating the potential of these transcription factors to acquiring new roles in different species.

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Section: Pif1a Is the Main Pif1 Homolog Regulating Many Other Processes Including Some That Are Not Regulated By Pifs In Arabidopsissupporting

confidence: 81%

Section: Loss Of Pif1a Might Results In Early Floweringmentioning

confidence: 99%

Conserved and newly acquired roles of PIF1 homologs in tomato (Solanum lycopersicum)

Simón-Moya

Barja

Morelli

et al. 2021

Preprint

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“…We predicted the interactions between PtrFRSs and other key genes, and a total of 11 PtrFRS genes appeared in the regulatory network, in addition to phyA, PIL5 and other key regulators of plant light response. phyA, an important factor in plant light regulation (Figure 5) [10], is in a regulatory relationship with six PtrFRS genes, and PIL5 and HY5 are also important regulators for light regulation in plants and had regulatory relationships with several PtrFRS genes [38,39]. PtrFRS5 interacted with two MYB proteins, which were homologous to AtMYB36.…”

Section: Potential Regulatory Of Ptrfrssmentioning

confidence: 99%

Genome-Wide Identification and Characterization of the FAR1/FHY3 Family in Populus trichocarpa Torr. & Gray and Expression Analysis in Light Response

Zhang

et al. 2021

Forests

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Light is an important environmental factor for plant growth, and in higher plants, phytochrome A (phyA) is the predominant far-red photoreceptor, involved in various photoresponses. The FAR1/FHY3 transcription factor family, derived from transposases, is able to regulate plant development in response to multiple photosensitizers phytochrome. In total, 51 PtrFRSs were identified in the poplar genome, and were divided into 4 subfamilies. Among them, 47 PtrFRSs are located on 17 chromosomes. Upstream cis-acting elements of the PtrFRS genes were classified into three categories: growth and metabolism, stress and hormone, and the hormone and stress categories contained most of the cis-acting elements. Analysis of the regulatory networks and expression patterns showed that most PtrFRSs responded to changes in light intensity and were involved in the regulation of phytochromes. In this study, 51 PtrFRSs were identified and comprehensively bioinformatically analyzed, and preliminary functional analysis and prediction of PtrFRSs was carried out.

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“…Arabidopsis PIF1 is unique as it is the only PIF that exclusively represses seed germination in the dark (Oh et al, 2004). The pif1 mutant also displays an early flowering phenotype (Wu et al, 2018). The wheat PIF1 homologue TaPIF-D1 is one of the genes with highest expression ( Figure 5A).…”

Section: Expression Of Pifs Mads-box Spl Epfl and Hd-zip Family Memmentioning

confidence: 99%

Ppd-1Remodels Spike Architecture by Regulating Floral Development in wheat

Liu

Zhang

Melzer

et al. 2020

Preprint

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The determination of spike architecture is critical to grain yield in wheat (Triticum aestivum), yet the underlying mechanisms remain largely unknown. Here, we measured 51 traits associated with spike architecture and floral development in 197 wheat accessions with photoperiod sensitive and insensitive alleles. We included five distinct allele combinations at the Photoperiod-1 (Ppd-1) loci. A systematic dissection of all recorded phenotypes revealed connections between floral development, spike architecture and grain yield. Modifying the durations of spikelet primordia initiation did not necessarily affect spikelet number. In addition, Ppd-1 loci clearly influenced rachis dry weight, pointing to the rachis vascular system as a potential target for higher yield. Ppd-1 displayed opposite effects on the durations of pre and post-anthesis phases. Ppd-1 controlled carpel size, but not anther size. Finally, the photoperiod-insensitive alleles of Ppd-1 triggered floral degeneration. In parallel, we profiled the spike transcriptome at six stages and four positions in three Ppd-1 genotypes which consists of 234 samples. Integrating phenotypic and expression data suggested that loss of function in Ppd-1 loci delayed floral degeneration by regulating autophagy and extended floret development by regulating genes in different families. Therefore, we concluded that Ppd-1 remodels spike architecture by regulating floral development in wheat. Appleyard, 1987). During WA stage, the meristematic dome initiates 7-9 floret primordia within each individual spikelet (1-3 primordia fewer than the maximum number) (Kirby and Appleyard, 1987). The lemmas of florets 1 and 2 at the base of the spikelet completely enclose the anther and ovary (Kirby and Appleyard, 1987). At the GA stage, the meristematic dome completes the initiation of floret primordia, so that after this stage no more floret primordia will be produced ; the glumes almost completely cover all but the tips of the florets. The spike length peaks at the YA stage (Guo et al., 2018); glumes are fully formed, and the lemmas of the first three florets are visible. At the TP stage, the first awn is visible (Zadoks et al., 1974), at HD stage, half of the spike emerges from the last leaf blade (Zadoks et al., 1974). Finally, at the AN stage, yellow anthers appear along the spike (Zadoks et al., 1974).Spike architecture is a complicated trait, that is determined by multiple factors. Spike fertility index (i.e. the ratio between grain number per spike and weight of spike chaff) is a critical metric of assimilate distribution between grains and spike chaff (e.g. glume, lemma, palea, rachis). Grain number per spikelet along the main inflorescence also influences spike shape. Spikelet density (the ratio between spike length and spikelet number) also indicates spike compactness. Spikelet fertility (the ratio between fertile and total spikelet number) informs on the proportion of aborted spikelets. In addition, grain size traits (thousand-kernel weight-TKW, grain area, grain width, grain le...

show abstract

PIL5 represses floral transition in Arabidopsis under long day conditions

Cited by 11 publications

References 44 publications

Conserved and newly acquired roles of PIF1 homologs in tomato (Solanum lycopersicum)

Conserved and newly acquired roles of PIF1 homologs in tomato (Solanum lycopersicum)

Genome-Wide Identification and Characterization of the FAR1/FHY3 Family in Populus trichocarpa Torr. & Gray and Expression Analysis in Light Response

Ppd-1Remodels Spike Architecture by Regulating Floral Development in wheat

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