Pineapple juice’s microbe as bioreceptor for detecting alcohol by electrochemical method

Iswantini, Dyah; Nurhidayat, Novik; Utami, Tri; Trivadila,; Soebrata, Betty Marita

doi:10.1088/1755-1315/187/1/012018

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…This is because the immobilization of SOD in zeolite for 24 h does not necessarily imply a lower concentration of SOD involved in the reaction with superoxide radicals when compared to prior research [100]. The detection of uric acid as a non-enzyme antioxidant using Lactobacillus plantarum immobilized using zeolite reported the results of electrode stability until day 18 [101]. Furthermore, the detection of antioxidants using superoxide dismutase-producing microorganisms from D. radiodurans bacteria as receptors immobilized on the surface of SPCE using electrochemical measurements, namely cyclic voltammetry, was carried out.…”

Section: Cell/microorganism-based Biosensorsmentioning

confidence: 99%

Antioxidant Determining Using Electrochemical Method

Sukma,

Iswantini,

Nurhidayat

et al. 2023

Chemistry

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Antioxidants are very beneficial for health as they protect the body from the effects of free radicals on various degenerative diseases caused by food contamination, air pollution, sunlight, etc. In general, methods for measuring the capacity of antioxidants generally use accurate methods such as spectrophotometry and chromatography. Still, this takes time, accurate sample preparation, and must be performed in a laboratory with particular expertise. Therefore, a new, more practical method needs to be developed for determining antioxidants, namely the electrochemical method. The electrochemical method is a promising method to develop because it comes with several advantages, including high sensitivity and fast response. The electrochemical method discussed in this article reviews sensors, biosensors, and nanosensors. This paper comprehensively analyzes contemporary developments in electrochemical biosensor techniques and antioxidant evaluation methodologies. The discussion centers on utilizing multiple biosensors. Electrochemical biosensors have been determined to be prevalent in analyzing food quality, assessing active factor functionality, and screening practical components. The present study outlines the difficulties linked with electrochemical bio-sensor technology and provides insights into the potential avenues for future research in this domain.

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Section: Cell/microorganism-based Biosensorsmentioning

confidence: 99%

Antioxidant Determining Using Electrochemical Method

Sukma,

Iswantini,

Nurhidayat

et al. 2023

Chemistry

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“…Susparini et al (2019) also examined an alcohol biosensor using Acetobacter aceti, producing the AOX enzyme with a sensitivity, LoD, and LoQ of 43.076 μA % −1 , 2.32 × 10 −5 , and 7.03 × 10 −5 % respectively. In addition, microbes from pineapple juice, namely Saccharomyces cerevisiae, produce the ADH enzyme, which can be used as an alcohol biosensor (Iswantini et al 2018). This tool was also developed using ADH microbes with a linear range of 0.05-2 mM (Istrate et al 2021).…”

Section: Introductionmentioning

confidence: 99%

The analytical performance of Saccharomyces cerevisiae and Bacillus megaterium microbial consortium as recognition element in ethanol biosensor

NURDIANI,

ISWANTINI,

NURHIDAYAT

et al. 2023

Biodiversitas

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Abstract. Nurdiani, Iswantini D, Nurhidayat N, Wahyuni WT, Kartono A. 2023. The analytical performance of Saccharomyces cerevisiae and Bacillus megaterium microbial consortium as recognition element in ethanol biosensor. Biodiversitas 24: 5928-5936. Alcohol, particularly ethanol, is commonly found in human food and plays a significant role in degenerative diseases and disability. Accurate measurement of alcohol in food products is essential to ensure adherence to the Muslim halal rule. However, existing alcohol biosensor that relies on a single microbe has limitations in measuring a wide range of ethanol concentrations. To address this issue, a microbial consortium is needed to expand the measurable range. Therefore, this study aimed to develop an innovative biosensor to widen the range of measured ethanol concentrations based on the microbial consortium of Saccharomyces cerevisiae YSAPMI.2 and Bacillus megaterium BSAPMI.1. The performance of the biosensor was evaluated using the cyclic voltammetry method. The results showed that the linear range, linearity, coefficient of determination, sensitivity, and response time, were 0.02-6.0%, 0.9968, 0.9936, 83.157 µA (%)-1, and 11 seconds. The LoD ??and LoQ theoretical values ??of the method obtained in the ethanol oxidation reaction were 0.060% and 0.182%, respectively. The confirmatory test for the LoD value of 0.01% yielded a positive response, while the confirmed LoQ value of 0.02% showed good precision and accuracy. The biosensor had precise %RSD values of 0.568, 1.338, and 4.632% for the high, medium, and low ethanol concentrations, respectively. The accuracy reflected as the recovery percentage was in the range of 90.27-111.07%. The biosensor was relatively specific and had no interferences with common ethanol compounds including methanol, sodium chloride, formic acid, and glucose. The stability obtained with biofilm showed a better result reaching 88% in 70 days. Based on the result, this microbial consortium biosensor could widen the range of measured ethanol concentrations and should be further developed to create a prototype for an accurate and practical analysis.

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Antioxidant Determining Using an Electrochemical Method

Sukma¹,

Iswantini²,

Nurhidayat³

et al. 2023

Preprint

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Antioxidants are very beneficial for health because they protect the body from the effects of free radicals on various degenerative diseases caused by food contamination, air pollution, sunlight, etc. In general, methods for measuring the capacity of antioxidants generally use accurate meth-ods such as spectrophotometry and chromatography. Still, it takes time, sample preparation and must be done in a laboratory with particular expertise. Therefore, a new, more practical method needs to be developed for determining antioxidants, namely the electrochemical method. The electrochemical method is promising to develop because it has several advantages, including high sensitivity and fast response. The electrochemical method discussed in this article reviews sensors, biosensors, and nanosensors. This paper comprehensively analyzes contemporary developments in electrochemical biosensor techniques and antioxidant evaluation methodologies. The discussion centers on utilizing multiple biosensors. Electrochemical biosensors have been determined to be prevalent in analyzing food quality, assessing active factor functionality, and screening practical components. The present study outlines the difficulties linked with electrochemical bio-sensor technology and provides insights into the potential avenues for future research in this domain.

show abstract

Pineapple juice’s microbe as bioreceptor for detecting alcohol by electrochemical method

Cited by 3 publications

References 10 publications

Antioxidant Determining Using Electrochemical Method

Antioxidant Determining Using Electrochemical Method

The analytical performance of Saccharomyces cerevisiae and Bacillus megaterium microbial consortium as recognition element in ethanol biosensor

Antioxidant Determining Using an Electrochemical Method

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