Pinoresinol Inhibits Proliferation and Induces Differentiation on Human HL60 Leukemia Cells

Sepporta, Maria Vittoria; Mazza, Teresa; Morozzi, Guido; Fabiani, Roberto

doi:10.1080/01635581.2013.828089

Cited by 28 publications

(22 citation statements)

References 44 publications

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“…Moreover, olive polyphenols have been reported to reduce glycaemia and cholesterolemia [13]. Olive oil polyphenols have a beneficial effect on the cancer cell line model: in vitro studies reveal that pinoresinol inhibits the proliferation of colon and prostate tumor cells and induces apoptosis in human leukaemia cells [14] Oleuropein, in addition, is capable of preventing colon rectal cancer in mice [15].…”

Section: Introductionmentioning

confidence: 99%

Dietary supplementation with olive mill wastewaters induces modifications on chicken jejunum epithelial cell transcriptome and modulates jejunum morphology

et al. 2018

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BackgroundThe Mediterranean diet is considered one of the healthier food habits and olive oil is one of its key components. Olive oil polyphenols are known to induce beneficial effects in several pathological conditions, such as inflammatory bowel disease, and to contrast the proliferation of cancer cells or hypercholesterolemia. Polyphenols are also present in waste products derived from the olive industry: olive mill wastewaters (OMWW) are rich in polyphenols and there is an increasing interest in using OMWW in animal nutrition. OMWW are attributed with positive effects in promoting chicken performance and the quality of food-derived products. However, a tissue-specific transcriptome target analysis of chickens fed with OMWW has never been attempted.ResultsWe explored the effect of dietary OMWW on the intestinal function in broilers. A morphological analysis of the jejunum revealed that OMWW reduced crypt depth, whereas no significant modifications were observed for villus height and the villus height/crypt depth ratio. An RNA Sequencing analysis was performed on isolated, intestinal, epithelial cells and 280 differentially expressed genes were found using a count-based approach. An enrichment analysis revealed that the majority of up regulated genes in the OMWW group were over-represented by the regulation of viral genome replication-related GO-Terms, whereas down regulated genes were mainly involved in cholesterol and lipid metabolism.ConclusionsOur study showed how an industrial waste product can be recycled as a feed additive with a positive relapse. OMWW dietary supplementation can be a nutritional strategy to improve chicken performance and health, prevent intestinal damage, enhance innate immunity and regulate cholesterol metabolism and fat deposition.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4962-9) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Dietary supplementation with olive mill wastewaters induces modifications on chicken jejunum epithelial cell transcriptome and modulates jejunum morphology

et al. 2018

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“…Sepporta et al [27] found that PINO inhibited the growth of MDA-MB-231, but not of MCF7 cells; however, their study was limited to 100 μM, which is not considered to be a physiological concentration. In contrast, we tested a wide range of PINO concentrations, ranging from 0.001 to 100 μM, and showed that low concentrations of PINO elicited a significant antiproliferative effect on both human breast tumour cell lines tested.…”

Section: Discussionmentioning

confidence: 99%

“…Other authors have reported anticancer effects of PINO by suppressing the expression of the lipogenic enzyme FASN in HER-2 overexpressing MCF7 cells [26]. Recently, Sepporta et al [27] observed that PINO inhibited the growth of MDA-MB-231 cells, but not of MCF7 cells. Importantly, no previous study has examined the effects of PINO on a normal human breast cell line, which would address whether PINO plays a protective role against cancer development.…”

Section: Introductionmentioning

confidence: 99%

Phytoestrogen (+)-pinoresinol exerts antitumor activity in breast cancer cells with different oestrogen receptor statuses

López-Biedma

Sánchez-Quesada

Beltrán

et al. 2016

BMC Complement Altern Med

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BackgroundConsumption of virgin olive oil (VOO) has been associated with a low breast cancer incidence. Pinoresinol is a phytoestrogen that is typically found in VOO. Considering the role of oestrogen in breast cancer development and progression, we investigated the potential antitumor activity of pinoresinol in breast cancer cells.MethodsTo address this question, we treated MDA-MB-231 (oestrogen receptor [ER] negative) and MCF7 (ER+) human breast tumour cells and MCF10A human mammary epithelial cells (ER-) with different concentrations of pinoresinol. The cytotoxic activity, cell proliferation, cell cycle profile, apoptosis induction, reactive oxygen species production and DNA damage were assessed.ResultsPinoresinol showed cytotoxic, anti-proliferative and pro-oxidant activity in human breast tumour cells, independent of their oestrogen receptor status. In addition, pinoresinol exerted antioxidant activity and prevented DNA damage associated with oxidative stress in human mammary epithelial cells.ConclusionsOverall, the results suggest that pinoresinol may have antitumor activity in human breast cancer cells independently of oestrogen receptor status. Furthermore, the results show that the pinoresinol has the typical characteristics of a chemopreventive compound.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1233-7) contains supplementary material, which is available to authorized users.

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“…PIN was reported to inhibit cell proliferation and induce the differentiation of leukemia HL60 cells by inducing mRNA and protein overexpression of the CDK inhibitor CDKN1A (Sepporta et al 2013). PIN-rich olive oil has been tested in p53 depleted cancer cells (SW480 with p53 mutant) and p53 competent cancer cells (RKO and HCT116).…”

Section: Dietary Lignansmentioning

confidence: 99%

Activation and Inhibition of ATM by Phytochemicals: Awakening and Sleeping the Guardian Angel Naturally

Farooqı

Tang

et al. 2015

Arch. Immunol. Ther. Exp.

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Double-stranded breaks (DSBs) are cytotoxic DNA lesions caused by oxygen radicals, ionizing radiation, and radiomimetic chemicals. Increasing understanding of DNA damage signaling has provided an ever-expanding list of modulators reported to orchestrate DNA damage repair and ataxia telangiectasia mutated (ATM) is the master regulator and main transducer of the DSB response. Increasingly, it is being realized that DNA damage response is a synchronized and branched network that functionalizes different molecular cascades to activate special checkpoints, thus temporarily arresting progression of the cell cycle while damage is being assessed and processed. It is noteworthy that both nutrigenetics and nutrigenomics have revolutionized the field of molecular biology and rapidly accumulating experimental evidence has started to shed light on biological activities of a wide range of phytochemicals reported to modulate cell cycle, DNA repair, cell growth, differentiation and apoptosis as evidenced by cell-based studies. In this review, we have attempted to provide an overview of DNA damage signaling, how ATM signaling regulates tumor necrosis factors-related apoptosis inducing ligand (TRAIL)-induced intracellular network. We also illuminate on how resveratrol, epigallocatechin gallate, curcumin, jaceosidin, cucurbitacin, apigenin, genistein, and others trigger activation of ATM in different cancer cells as well as agents for ATM inactivation. Understanding the interplay of TRAIL-induced intracellular signaling and ATM modulation of downstream effectors is very important. This holds particularly for a reconceptualization of the apparently paradoxical roles and therapeutically targetable for enhancing the response to DNA damage-inducing therapy.

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Pinoresinol Inhibits Proliferation and Induces Differentiation on Human HL60 Leukemia Cells

Cited by 28 publications

References 44 publications

Dietary supplementation with olive mill wastewaters induces modifications on chicken jejunum epithelial cell transcriptome and modulates jejunum morphology

Dietary supplementation with olive mill wastewaters induces modifications on chicken jejunum epithelial cell transcriptome and modulates jejunum morphology

Phytoestrogen (+)-pinoresinol exerts antitumor activity in breast cancer cells with different oestrogen receptor statuses

Activation and Inhibition of ATM by Phytochemicals: Awakening and Sleeping the Guardian Angel Naturally

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