Placental histological on preeclamptic rats (<i>Rattus norvegicus</i>) after administration of nanoherbal haramonting (<i>Rhodomyrtus tomentosa</i>)

Irianti, Evi; Ilyas, Syafruddin; Hutahaean, Salomo; Rosidah,; Situmorang, Putri Cahaya

doi:10.5958/0974-360x.2020.00686.1

Cited by 10 publications

(8 citation statements)

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“…This Plant like Morinda citrifolia plays a pivotal role in controlling blood pressure (Purwaningroom et al, 2021). This research supports the findings of Irianti et al (2020), which indicated that haramonting can lower the blood pressure in pregnant rats with preeclampsia while increasing the trophoblast count and improving placental histology. NTR treatment resulted in a significant difference in the birth weight of hypertensive rats (P < 0.01).…”

Section: Resultssupporting

confidence: 83%

“…Pregnancy was confirmed by a vaginal plug, and the day was defined as day 0. On gestational day 6, systemic inflammation was induced in pregnant rats by infusing Escherichia coli lipopolysaccharide (LPS) into the peritoneal cavity using a small osmotic pump (Irianti et al, 2020). 4.…”

Section: Animal Handlingmentioning

confidence: 99%

“…The slides were dehydrated with 70%, 96%, and 100% alcohol, immersed in xylol, and placed on a cover glass. The sample was examined using a light microscope with 5 times the field of view (Irianti et al, 2020).…”

Section: Hematoxylin-eosin (Hande) Stainingmentioning

confidence: 99%

“…Haramonting leaves have antioxidant activity in vitro and in vivo (Jeong et al, 2013). Antioxidants from this plant can inhibit lipid peroxidation; improve the histology of the placenta, testes, and diabetic wounds; increase the expression of HSP70; and increase the ability to reduce free radicals (Ilyas & Situmorang, 2021;Ilyas et al, 2020;Situmorang et al, 2018a;Irianti et al, 2020). The high levels of phenolic compounds impart high antioxidant capacity; thus, haramonting is a potential source of health-promoting compounds (Zhang et al, 2018).…”

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confidence: 99%

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Suppression of Fas expression in the hypertensive placental histology of rast given Nano herbal of Rhodomyrtus tomentosa leaves

Situmorang

Syahputra

Simanullang³

2022

KJS publishes peer-review articles in Mathematics, Computer Sci

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High blood pressure during pregnancy can signal a high-risk pregnancy and result in difficulties. The expression of Fas is critical for maintaining hypertensive immunity. Fas is prevalent in the trophoblast and enhances maternal-derived apoptosis. Because it contains acylphloroglucinol, flavonoids, tannins, and triterpenes, Rhodomyrtus tomentosa (haramonting) is commonly used in traditional Indonesian medicine to treat high blood pressure. This study aimed to investigate and determine the role of haramonting in lowering Fas expression in hypertensive rats’ placental histopathology. The rats were confirmed to be pregnant and divided into five groups: normal pregnant rats (control); hypertensive rats without treatment; and hypertensive rats given nanoherbal Rhodomyrtus tomentosa (NRT) at different doses: 100 mg/kg body weight (BW), 200 mg/kg BW, and 400 mg/kg BW. On gestation day 20, pregnant rats were euthanized under ketamine anaesthesia. Enzyme-linked immunosorbent assay (ELISA) analysis was used to examine the expression of the HSP family. Immunohistochemistry was used to assess Fas expression. In hypertensive rats, NRT lowered the systolic and diastolic blood pressure (P < 0.01), and NRT improved placental efficiency and restored birth weight. In hypertensive rats, higher doses of NRT were associated with higher levels of HSP27, HSP70, and HSP90. In placental histology, NRT suppresses Fas expression, acting as an anti-apoptotic agent in trophoblast cells. Because NRT is high in antioxidants and protects cells from hypoxia and dehydration, it suppresses Fas activity in the labyrinthine zone, basal zone, and yolk sac.

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Section: Resultssupporting

confidence: 83%

Section: Animal Handlingmentioning

confidence: 99%

Section: Hematoxylin-eosin (Hande) Stainingmentioning

confidence: 99%

mentioning

confidence: 99%

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Suppression of Fas expression in the hypertensive placental histology of rast given Nano herbal of Rhodomyrtus tomentosa leaves

Situmorang

Syahputra

Simanullang³

2022

KJS publishes peer-review articles in Mathematics, Computer Sci

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“…R. tomentosa can also reduce HIF1α better in trophoblast cells aged 8 weeks compared to Zanthoxylum acanthopodium (Situmorang et al, 2022). These plants can decrease lipid peroxidation, enhance the expression of HSP-70, boost the capacity to scavenge free radicals, and improve the histology of the placenta, testis, and lungs (Ilyas and Situmorang, 2021;Ilyas et al, 2019;Irianti et al, 2020;Situmorang and Ilyas, 2018;Situmorang et al, 2020) Due to the high amount of phenolic. It has a strong antioxidant capacity and the potential to be a source of substances that improve health (Vo and Ngo, 2019).…”

Section: Important Angiogenesis Regulators Vegf Andmentioning

confidence: 99%

Effect of Rhodomyrtus tomentosa Hassk. on HIF1α and VEGF expressions on hypertension placental

Situmorang¹,

Ilyas²,

Siahaan³

et al. 2022

J Pharm Pharmacogn Res

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Context: HIF1α and VEGF are proteins marker oxidative stress and a decrease in placental growth factor (PlGF). Decreasing of HIF1α and VEGF in rats displayed poor trophoblast differentiation, placental abnormalities, and fetal mortality. Rhodomyrtus tomentosa is a flowering plant in the Myrtaceae family that has the potential to be a source of health-promoting chemicals. Aims: To analyze HIF1α and VEGF in serum and hypertension placental tissue after giving Rhodomyrtus tomentosa (RHO) leaves extract. Methods: Six treatments were given to the rats that were identified as being pregnant and pregnant rats with hypertension were given RHO with three doses: (a) normal pregnant rats (control); (b) hypertensive rats; (c) hypertensive rats + 100 mg/kg BW of RHO; (d) hypertensive rats +200 mg/kg BW of RHO; and (e) hypertensive rats + 400 mg/kg BW of RHO and (f) hypertensive rats + nifedipine. Under ketamine anesthesia, pregnant rats were removed on their 20th day of gestation. Immunohistochemistry and ELISA were used to assess HIF1α and VEGF protein expression. Results: There was a significant difference (p<0.01) in the expression of HIF1α and VEGF in the labyrinthine zone and yolk sac of the rat placenta between the normal (C-) and hypertensive (C+) groups. HIF1α and VEGF expression decreased when RHO was administered at doses ranging from 100 to 400 mg/kg BW. However, there was no significant change (p>0.05) in VEGF expression in the basal zone of the rat placenta across all groups. Conclusions: Rhodomyrtus tomentosa leaves extract decreases HIF1α and VEGF expressions in serum and repairs the tissue of the placenta's labyrinth, basal, and yolk sacs.

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The Effect of Giving Nanoherbal of Biwa (Eriobotrya japonica (Thunb.) Lindl. (Thunb.) Lindl.) Leaf on the Quality and Quantity of Spermatozoa And Microstructure in Diabetes Rat (Rattus norvegicus) Induced by Alloxan

Ilyas

2022

Int J Ecophysiol

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One of causes in decreasing quantity and quality of spermatozoon is disease. Increased blood glucose levels can trigger demage of spermatogenesis in the testes, thereby reducing spermatozoa production. Biwa leaves (Eriobotrya japonica (Thunb.) Lindl.) contain triterpenes which are expected to overcome blood glucose levels and quantity of spermatozoa in the cauda epididymis. To investigate this problem, an experimental test of nano herbal of biwa leaves was carried out in a rat model (Rattus norvegicus) diabet. There are five treatments and six replications, it was found that the best dose of 500 mg/kg body weight could improve spermatogenesis so that the number of rat spermatozoa remained normal. It was concluded that nano herbal of biwa leaves can be used as an alternative drug to maintain fertility as seen from spermatogenesis and the number of spermatozoa. The result is analyzed by ANOVA test and further by Post-Hoc-Test (Duncan). The result show the significant differences (p<0,05) to quantity and quality of spermatozoon of adult white male mouse (Rattus norvegicus).

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Placental histological on preeclamptic rats (Rattus norvegicus) after administration of nanoherbal haramonting (Rhodomyrtus tomentosa)

Cited by 10 publications

References 0 publications

Suppression of Fas expression in the hypertensive placental histology of rast given Nano herbal of Rhodomyrtus tomentosa leaves

Suppression of Fas expression in the hypertensive placental histology of rast given Nano herbal of Rhodomyrtus tomentosa leaves

Effect of Rhodomyrtus tomentosa Hassk. on HIF1α and VEGF expressions on hypertension placental

The Effect of Giving Nanoherbal of Biwa (Eriobotrya japonica (Thunb.) Lindl. (Thunb.) Lindl.) Leaf on the Quality and Quantity of Spermatozoa And Microstructure in Diabetes Rat (Rattus norvegicus) Induced by Alloxan

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