Plant-derived compounds: A potential source of drugs against Tobacco mosaic virus

Cai, Lin; Zhang, Wang; Jia, Huanyu; Feng, Hui; Wei, Xuefeng; Chen, Haitao; Wang, Daibin; Yang, Xue; Sun, Xianchao

doi:10.1016/j.pestbp.2020.104589

Cited by 19 publications

(14 citation statements)

References 44 publications

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“…49 Studies have shown that the expression of plant defense-related enzymes such as PAL, POD and SOD can improve the resistance and tolerance of plants to TMV. 20,21 Importantly, SOD and POD are prominent antioxidant defense enzymes involved in the formation and catabolism of ROS, 50 which is an important signaling molecule that regulates downstream gene expression, antioxidant enzyme system and stomatal movement, thereby improving plant adaptation to stress as well as promoting resistance to pathogen infection. 30,51 Hydrogen peroxide is a relatively stable type of ROS in plants 52 and has been showed to induce RNAdependent RNA polymerase 1 mediated RNA silencing in tobacco and Arabidopsis thaliana to suppress systemic infection of TMV.…”

Section: Discussionmentioning

confidence: 99%

“…18,19 Polysaccharide peptides, lentinan and ursolic acid can promote the expression of defense-related enzymes, such as phenylalanine ammonia-lyase (PAL) and peroxidase (POD), as well as defense-related genes in host plants, thus increasing the resistance and tolerance of plants to TMV. 20,21 Studies have shown that quercetin nanoliposomes can inhibit TMV by specifically inhibiting the expression of Hsp70 in N. benthamiana. 22 In addition, a polysaccharide was found in Dendrobium nobile, which can stimulate calcium signaling pathway and promote host defense against TMV infection.…”

Section: Introductionmentioning

confidence: 99%

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Antiviral modes of action of the novel compound GLY‐15 containing pyrimidine heterocycle and moroxydine skeleton against tobacco mosaic virus

Guo

et al. 2022

Pest Management Science

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BACKGROUND Plant virus diseases are difficult to prevent and control, causing serious economic losses to the agricultural production world. To develop new pesticides with antiviral activity, a serial of compounds containing the structure of pyrimidine and moroxydine were synthesized, among which GLY‐15 exhibited good antiviral activity against tobacco mosaic virus (TMV), while the mechanism of antiviral activity remains to be clarified. RESULTS GLY‐15 treatment significantly inhibited the formation of necrotic spots caused by TMV in Nicotiana glutinosa, and effectively suppressed the systemic transportation of TMV expressing a reporter gene (p35S‐30B:GFP) in N. benthamiana and markedly reduced the accumulation of a movement deficient TMV in plants as well as viral RNA accumulation in tobacco protoplasts. The results of RNA sequencing showed that GLY‐15 induced significant differential expression of genes or pathways involved in the stress response, defense response and signal transduction, phytohormone response and metabolism. Among them, real‐time quantitative PCR validated that the expression of 12 critical genes such as heat shock protein, receptor kinase, cell‐wall‐related protein, disease‐related protein and glucan endo‐1,3‐β‐glucosidase were significantly up‐regulated. In addition, GLY‐15 triggered reactive oxygen species (ROS) production and induced the activity of several crucial defense related enzymes in plants. The results of molecular docking showed potential binding ability of GLY‐15 with TMV helicase and the coat protein. CONCLUSION This study provide valuable insights into antiviral mechanism of action for GLY‐15, which is expected to be applied as a pesticide for the management of plant viruses. © 2022 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antiviral modes of action of the novel compound GLY‐15 containing pyrimidine heterocycle and moroxydine skeleton against tobacco mosaic virus

Guo

et al. 2022

Pest Management Science

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“…Natural products play a significant role in the development of green pesticides with novel structure and unique mechanism of action. − In recent decades, several natural product-based antiviral agents, such as dufulin, NK-007, and xiangcaoliusuobingmi, (Figure ) have been developed. In addition, many natural products or their analogues, such as (tetrahydro)harmine, , coumarin, phenols, gossypols, flavonoids, limonoids, , and lignans, − have also been found to possess significant antiviral activities.…”

Section: Introductionmentioning

confidence: 99%

Discovery of Novel α-Methylene-γ-Butyrolactone Derivatives Containing Vanillin Moieties as Antiviral and Antifungal Agents

Wang

Zhang

et al. 2022

J. Agric. Food Chem.

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On the basis of the structure of nicotlactone A (L1), a series of novel α-methylene-γ-butyrolactone derivatives B1–B43 were designed and synthesized by structure simplification and active fragment replacement strategies, and their antiviral and antifungal activities were evaluated. The bioassay studies indicated that many target compounds possessed good to excellent antiviral activity against tobacco mosaic virus (TMV) and some of these compounds exhibited specific antifungal activities against Valsa mali and Fusarium graminearum. Compound B32 exhibited the best anti-TMV activity (inactivation effect, 88.9%; protection effect, 65.8%; curative effect, 52.8%) in vivo at 500 mg/L, which is significantly higher than that of commercial virucides ribavirin and ningnanmycin. The inhibition effect of compound B32 was also visualized by the inoculation test using green fluorescent protein (GFP)-labeled TMV. The preliminary antiviral mechanism of compound B32 was investigated. Transmission electron microscopy (TEM) showed that compound B32 could destroy the integrity of virus particles. Then, molecular docking and isothermal titration calorimetry (ITC) analysis further demonstrated that compound B32 exhibited a strong binding affinity to the TMV coat protein with a dissociation constant (K d) of 3.06 μM, superior to ribavirin. Thus, we deduced that compound B32 may interfere with the self-assembly of TMV particles by binding TMV coat protein (CP). In addition, compound B28 showed good in vitro activity against F. graminearum with an inhibition rate of 90.9% at 50 mg/L, which was greater than that of fluxapyroxad (59.1%) but lower than that of the commercial fungicide carbendazim (96.8%). The present study provides support for the application of these α-methylene-γ-butyrolactone derivatives as novel antiviral and antifungal agents in crop protection.

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“…Biopesticides are regarded as remarkable alternatives to classic agrochemicals, and have played a crucial role in plant disease control. Renewable plant bioresources contain a variety of plantderived compounds (PDCs) (Hassan et al, 2009;Li et al, 2014;Cai et al, 2020). Recent studies have proven that these compounds can be used as antibacterial agents to suppress soil borne pathogens and promote plant growth (Paret et al, 2010;Abo-Elyousr et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Preliminary Studies on the Antibacterial Mechanism of a New Plant-Derived Compound, 7-Methoxycoumarin, Against Ralstonia solanacearum

et al. 2021

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Ralstonia solanacearum (R. solanacearum) is one of the most devastating plant bacterial pathogens and leads to serious economic losses in crops worldwide. In this study, the antibacterial mechanism of 7-methoxycoumarin, a new coumarin antibiotic, was preliminarily investigated by the observation of symptoms and physical and biochemical analyses. The results showed that 7-methoxycoumarin significantly suppressed bacterial growth of R. solanacearum, with the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) values of 75 and 175 mg/L, respectively. Electron microscopy observations showed that the bacterial cell membrane was destroyed after 7-methoxycoumarin treatment. Biofilm formation of R. solanacearum was significantly suppressed by 7-methoxycoumarin at concentrations ranging from 25 to 100 mg/L. Furthermore, virulence-associated genes epsE, hrpG, and popA of R. solanacearum were significantly inhibited by 7-methoxycoumarin. The application of 7-methoxycoumarin effectively suppressed tobacco bacterial wilt progress in pot experiments, with relative control efficiencies of 83.61, 68.78, and 58.11% at 6, 8, and 10 days post inoculation, respectively.

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Plant-derived compounds: A potential source of drugs against Tobacco mosaic virus

Cited by 19 publications

References 44 publications

Antiviral modes of action of the novel compound GLY‐15 containing pyrimidine heterocycle and moroxydine skeleton against tobacco mosaic virus

Antiviral modes of action of the novel compound GLY‐15 containing pyrimidine heterocycle and moroxydine skeleton against tobacco mosaic virus

Discovery of Novel α-Methylene-γ-Butyrolactone Derivatives Containing Vanillin Moieties as Antiviral and Antifungal Agents

Preliminary Studies on the Antibacterial Mechanism of a New Plant-Derived Compound, 7-Methoxycoumarin, Against Ralstonia solanacearum

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