Plant pathogenic members of the genera Acidovorax and Herbaspirillum

Fegan, Mark

doi:10.1007/978-1-4020-4538-7_18

Cited by 7 publications

(14 citation statements)

References 83 publications

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“…Genome C72, from a novel species of Eubacterium , was found almost exclusively in dental calculus, and these bacteria may have been a component of a biofilm associated with periodontal disease, as are other Eubacterium species [ 16 ]. Genome C18 belongs to Acidovorax , which is associated with plant pathogens [ 17 ] and may have been introduced with the wood chips that covered the skeleton. We therefore decided to reconstruct the Ragna genome by read mapping against its close relatives within S. enterica .…”

Section: Resultsmentioning

confidence: 99%

“…In support of this role, C72 was recovered almost exclusively from dental calculus whereas reads from the putative environmental taxa were present in multiple sources (Concoct 5, see Key Resources Table ). C18 belongs to Acidovorax , which is associated with plant pathogens [ 17 ], and may have been introduced with the wood chips that covered the skeleton.…”

Section: Methodsmentioning

confidence: 99%

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Pan-genome Analysis of Ancient and Modern Salmonella enterica Demonstrates Genomic Stability of the Invasive Para C Lineage for Millennia

et al. 2018

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SummarySalmonella enterica serovar Paratyphi C causes enteric (paratyphoid) fever in humans. Its presentation can range from asymptomatic infections of the blood stream to gastrointestinal or urinary tract infection or even a fatal septicemia [1]. Paratyphi C is very rare in Europe and North America except for occasional travelers from South and East Asia or Africa, where the disease is more common [2, 3]. However, early 20th-century observations in Eastern Europe [3, 4] suggest that Paratyphi C enteric fever may once have had a wide-ranging impact on human societies. Here, we describe a draft Paratyphi C genome (Ragna) recovered from the 800-year-old skeleton (SK152) of a young woman in Trondheim, Norway. Paratyphi C sequences were recovered from her teeth and bones, suggesting that she died of enteric fever and demonstrating that these bacteria have long caused invasive salmonellosis in Europeans. Comparative analyses against modern Salmonella genome sequences revealed that Paratyphi C is a clade within the Para C lineage, which also includes serovars Choleraesuis, Typhisuis, and Lomita. Although Paratyphi C only infects humans, Choleraesuis causes septicemia in pigs and boar [5] (and occasionally humans), and Typhisuis causes epidemic swine salmonellosis (chronic paratyphoid) in domestic pigs [2, 3]. These different host specificities likely evolved in Europe over the last ∼4,000 years since the time of their most recent common ancestor (tMRCA) and are possibly associated with the differential acquisitions of two genomic islands, SPI-6 and SPI-7. The tMRCAs of these bacterial clades coincide with the timing of pig domestication in Europe [6].

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Pan-genome Analysis of Ancient and Modern Salmonella enterica Demonstrates Genomic Stability of the Invasive Para C Lineage for Millennia

et al. 2018

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“…En relación con los géneros Erwinia y Acidovorax, la mayoría de sus miembros son considerados fitopatógenos (Fegan, 2006;Kado, 2006), y su permanencia luego del proceso de desinfección podría considerarse un aspecto negativo, sin embargo, no fue posible establecer una asociación patogénica con vainilla. Esta condición la presentan algunas especies que se encuentran con frecuencia en el suelo, agua y como endófitas, las cuales son incapaces de ejercer acción patogénica en plantas (Fegan, 2006).…”

Section: Discussionunclassified

Desinfección de segmentos nodales sobre el rendimiento morfogenético de vitroplantas de Vanilla planifolia Andrews

et al. 2019

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Introducción. El establecimiento aséptico en condiciones de laboratorio de parientes silvestres de Vanilla planifolia, no ha sido documentado, así como tampoco se ha evaluado la importancia de la desinfección en el aumento del vigor. Objetivo. El objetivo de este trabajo fue evaluar la respuesta morfogenética de vitroplantas de V. planifolia a la desinfección en invernadero y en laboratorio, durante el establecimiento y multiplicación in vitro. Materiales y métodos. El estudio se realizó durante el 2015 y 2016 en el Laboratorio de Cultivo de Tejidos del Instituto de Investigación y Servicios Forestales (INISEFOR) de la Universidad Nacional (UNA), Heredia, Costa Rica. El medio empleado fue el cultivo Murashige y Skoog (MS). Se evaluaron seis tratamientos: 1) alcohol 70%/NaClO 0,35%; 2) kilol/NaClO 0,35%; 3) testigo/NaClO 0,35%; 4) alcohol 70%/HgCl2 0,2%; 5) kilol/HgCl2 0,2% y 6) testigo/HgCl2 0,2%. Las variables que se evaluaron fueron: porcentaje de contaminación total, por bacterias y hongos; longitud, peso y diámetro del brote; número, longitud y peso de las raíces. Resultados. Los géneros de bacterias y hongos más dominantes fueron Pantoea sp. (33%) y Fusarium sp. (12%). El mejor tratamiento de desinfección se obtuvo con el uso de kilol en invernadero y HgCl2 en laboratorio, donde se logró un 100% de explantes libres de contaminantes. Este mismo tratamiento presentó valores significativamente superiores para la longitud y peso del brote, número y peso de las raíces, durante el establecimiento. En la etapa de multiplicación, la condición aséptica de los grupos experimentales presentó valores significativamente mayores para las variables longitud y peso del brote comparado con la respuesta morfogénica presentada por las vitroplantas verdes y vigorosas en la condición de contaminación. Conclusión. La secuencia de tratamientos de desinfección representa la línea base para la optimización de protocolos de cultivo in vitro de parientes silvestres de V. planifolia, constituye un aporte para la conservación ex situ de materiales únicos y sirve para incrementar material genético promisorio.

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“…The bacterium enters sugarcane leaves through the stomata and invades the intercellular spaces, but it does not reach the xylem and phloem vessels [ 46 ]. Despite of the losses of productivity caused by the infection with A. avenae in sugarcane, little is known about the molecular mechanisms triggered in sugarcane by this disease [ 7 ]. Data generated from the characterization of sRNAs and the regulatory network of the host’s immune systems need to be explored in order to develop tools to enhance plant resistance against this pathogen [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

“…This bacterium was previously classified as Pseudomonas, and recently reclassified to the Acidovorax genus [ 6 ]. In sugarcane, A. avenae causes the Red Stripe Disease, which damages leaves and leaf sheaths [ 7 ]. Despite of the harm caused by the disease, little is known about the molecular mechanisms triggered in sugarcane in response to the infection.…”

Section: Introductionmentioning

confidence: 99%

Roles of Non-Coding RNA in Sugarcane-Microbe Interaction

Thiebaut

Rojas

Grativol

et al. 2017

ncRNA

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Studies have highlighted the importance of non-coding RNA regulation in plant-microbe interaction. However, the roles of sugarcane microRNAs (miRNAs) in the regulation of disease responses have not been investigated. Firstly, we screened the sRNA transcriptome of sugarcane infected with Acidovorax avenae. Conserved and novel miRNAs were identified. Additionally, small interfering RNAs (siRNAs) were aligned to differentially expressed sequences from the sugarcane transcriptome. Interestingly, many siRNAs aligned to a transcript encoding a copper-transporter gene whose expression was induced in the presence of A. avenae, while the siRNAs were repressed in the presence of A. avenae. Moreover, a long intergenic non-coding RNA was identified as a potential target or decoy of miR408. To extend the bioinformatics analysis, we carried out independent inoculations and the expression patterns of six miRNAs were validated by quantitative reverse transcription-PCR (qRT-PCR). Among these miRNAs, miR408—a copper-microRNA—was downregulated. The cleavage of a putative miR408 target, a laccase, was confirmed by a modified 5′RACE (rapid amplification of cDNA ends) assay. MiR408 was also downregulated in samples infected with other pathogens, but it was upregulated in the presence of a beneficial diazotrophic bacteria. Our results suggest that regulation by miR408 is important in sugarcane sensing whether microorganisms are either pathogenic or beneficial, triggering specific miRNA-mediated regulatory mechanisms accordingly.

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Plant pathogenic members of the genera Acidovorax and Herbaspirillum

Cited by 7 publications

References 83 publications

Pan-genome Analysis of Ancient and Modern Salmonella enterica Demonstrates Genomic Stability of the Invasive Para C Lineage for Millennia

Pan-genome Analysis of Ancient and Modern Salmonella enterica Demonstrates Genomic Stability of the Invasive Para C Lineage for Millennia

Desinfección de segmentos nodales sobre el rendimiento morfogenético de vitroplantas de Vanilla planifolia Andrews

Roles of Non-Coding RNA in Sugarcane-Microbe Interaction

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