Plant regeneration from callus cultures of Morus indica L. derived from seedlings and mature plants

Sahoo, Y.; Pattnaik, Swapnajit; Chand, Pradeep K.

doi:10.1016/s0304-4238(96)00980-6

Cited by 17 publications

(9 citation statements)

References 19 publications

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“…For example, we observed that, depending on the callus induction media selected, the average number of shoots was significantly higher in calli obtained from internodal explants than from leaf explants. Such fluctuations in media response by different explant types also have been reported for Lonicera japonica (Georges et al, 1993), Morus indica (Sahoo et al, 1997), and Gladiolus sp. Differences were significant among the different explant types and callus induction media with respect to the number of shoots produced per callus/explant (Table 1 ).…”

Section: Shoot Formation and Multiplicationsupporting

confidence: 70%

“…To conclude, a callus-mediated plant regeneration protocol is a critical requirement because it allows researchers to exploit techniques for in-vitro selection, somaclonal variation, and genetic engineering in efforts to improve plants (Sahoo et al, 1997). Howeveb genetic mutations are more frequent in shoots regenerated from calli than from other explant types, particularly when the sub-culturing period is prolonged, because of the non-uniform nature of callus tissue (Kantia and Kothari, 2002).…”

Section: Rooting and Acclimatizationmentioning

confidence: 99%

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Direct and indirect regeneration of plants from internodal and leaf expiants ofHypencum bupleuroides gris

2007

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Species of the genus Hypericum are of considerable interest worldwide because of their medicinal properties. In-vitro culture is a useful tool for both multiplication of the genus and studying its economically important secondary metabolites. Here, we present an effective in-vitro propagation method for H. bupleuroides. Leaf and internodal explants excised from 9-week-old, in vitro-germinated seedlings were cultured on a Murashige and Skoog (MS) medium supplemented with benzyladenine (BA; 1.0 or 0.1 mg L -1) and 2,4-dichlorophenoxyacetic acid (2,4-D; 1.0 or 0.1 mg IL 1). Depending on the BA and 2,4-D combination used, these cultures produced adventitious shoot buds directly on the surfaces of both types of explants as well as excessive calli. Numerous shoots were obtained when the calli from both explant types were cultured on an MS medium supplemented with 2 mg L 1 BA. Internodal explants were more responsive than leaf tissues to direct and indirect plant regeneration. After shoots that regenerated from either the calli or the explant surface were excised, rooting was best on an MS medium lacking any growth hormones. These rooted plants were then acclimatized under greenhouse conditions, and 90% of regenerants had survived. Ours is the first report of in-vitro plant regeneration from H. bupleuroides.

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Section: Shoot Formation and Multiplicationsupporting

confidence: 70%

Section: Rooting and Acclimatizationmentioning

confidence: 99%

Direct and indirect regeneration of plants from internodal and leaf expiants ofHypencum bupleuroides gris

2007

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“…On the other hand, Sahoo et al (1997) observed that the concentrations of 2,4-D between 0.5 and 4.0 mg L -1 were not effective on inducing calogenesis in leaf explants of mulberry (Morus indica L.). According to Grattapaglia & Machado (1998), 2,4-D tends to stimulate callus formation, even at low concentrations.…”

Section: Resultsmentioning

confidence: 98%

Callus induction in leaf segments of Croton urucurana Baill

Lima

Paiva

Nogueira³

et al. 2008

Ciênc. agrotec.

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RESUMOA sangra d água, espécie pertencente à família Euphorbiaceae, apresenta potencial na recuperação de matas ciliares degradadas e é extensamente utilizada na medicina tradicional, como cicatrizante e no tratamento de reumatismos. Entretanto, suas sementes apresentam dormência e baixa viabilidade, dificultando a propagação desta espécie. Avaliou-se o efeito de diferentes concentrações de 2,4-D e BAP ou TDZ e de ANA e BAP, com suas possíveis combinações, na indução de calos em segmentos foliares de sangra d água. Quarenta e cinco dias após a inoculação, foi avaliado o peso fresco dos calos. O uso de BAP isoladamente e a combinação entre ANA e BAP não promoveram calogênese em segmentos foliares de sangra d água. Embora a combinação de 2,4-D e BAP ou TDZ tenha induzido a formação de calos, o uso isolado de 2,4-D proporcionou o maior peso fresco destes.Termos para indexação: Micropropagação, calogênese, cultura de tecidos. ABSTRACTCroton urucurana Baill., a species belonging to the family Euphorbiaceae, can be useful in the recovery of degraded riparian areas. In the traditional medicine, it is widely used as cicatrizant and in the treatment of rheumatism. However, its seeds present dormancy and low viability, making the propagation of this species a challenge. With the objective of establishing an alternative route for the propagation, the effect of different concentrations of 2,4-D (2,4-dichlorophenoxyacetic acid) with BAP (6-benzylaminopurine) or TDZ (thidiazuron) and of NAA (1-naphthaleneacetic acid) with BAP and their possible combinations were evaluated for callus induction in leaf segments. Callus fresh mass was evaluated forty-five days after inoculation. The isolated use of BAP and the combination of NAA with BAP did not promote calogenesis in leaf segments. Even though the combination of 2,4-D with BAP or TDZ had induced the formation of callus, it was the isolated use of 2,4-D that provided the highest callus fresh mass.

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“…For example, evaluating different nutritive medium in calli cultures from leaf segments of Morus indica L. it was found that the MS media does not produce callogenesis (Sahoo et al, 1997); Nevertheless, in studies with adult leaves of Paulownia elongata S., Y. Hu obtained a higher proportion of callus with morphogenic response in the MS media than in the WPM media (Castellanos et al, 2006). In this manner, the most appropriate culture media for a determined response depends, among other factors, on the species.…”

Section: Resultsmentioning

confidence: 99%

Effect of Culture Medium in Callogenesis from Adult Walnut Leaves (Juglans regia L.)

Avilés¹,

Ríos²,

González³

et al. 2009

Chilean J. Agric. Res.

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To define preliminary aspects of walnut (Juglans regia L.) callogenesis in indirect propagation systems, this work analyzed callogenic induction in adult leaves of walnut in broadleaf trees (BTM), Murashige and Skoog (MS) Driver Kuniyuki Walnut (DKW) and Woody Plant (WPM) media. Leaf segments of 1 cm 2 , previously sterilized, were placed in each culture medium, supplemented with 21.4 µM naphtalene acetic acid (NAA) and 8.8 µM 6-benzylaminopurine (BAP). A completely random design was used; the experimental unit was a Petri dish with six callus explants. Each treatment was composed of seven Petri dishes. Callogenesis (%), nodular calli (%) and histology of calli explants were evaluated at 4 weeks by staining 10 µm slices with safranine-fastgreen. There were no significant differences (α = 0.05) in callogenesis among evaluated media; but nodular calli percentages were significantly higher in BTM (75%) and WPM (63%). Only in calli obtained in the BTM medium did the histology show the presence of meristematic zones, but without external expression. These results indicate that the medium influences the morphogenic characteristics of resultant callus. Calli produced in BTM presented better morphogenic potential, with meristems at primary stages of organization.

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Plant regeneration from callus cultures of Morus indica L. derived from seedlings and mature plants

Cited by 17 publications

References 19 publications

Direct and indirect regeneration of plants from internodal and leaf expiants ofHypencum bupleuroides gris

Direct and indirect regeneration of plants from internodal and leaf expiants ofHypencum bupleuroides gris

Callus induction in leaf segments of Croton urucurana Baill

Effect of Culture Medium in Callogenesis from Adult Walnut Leaves (Juglans regia L.)

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