Plant regeneration from cotyledons and embryonic axes in apple: Sites of reaction and effect of pre-culture in the light

Keulemans, Johan; Witte, K De

doi:10.1007/bf02551476

Cited by 5 publications

(3 citation statements)

References 13 publications

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“…Höfer (1994) transferred embryo-derived anthers of apple to MS medium with 0.5 µM TDZ and succeeded in obtaining secondary embryogenesis and adventitious shoots. Cotyledons or the embryonic axis derived from embryos isolated from seeds of apple (Keulemans and de Witte, 1994;Kouider et al, 1984) and pear (Browning et al, 1987) regenerated adventitious shoots. Optimal conditions for shoot differentiation from cotyledons and the embryonic axis should be further examined.…”

Section: Discussionmentioning

confidence: 99%

Plant Regeneration from Anther-derived Embryos of Apple and Pear

Kadota¹,

Han²,

Niimi³

2002

HortSci

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Anthers of six apple [Malus ×domestica (L.) Borkh.], three Japanese pear (Pyrus pyrifolia N.) and two European pear (Pyrus communis L.) scion cultivars were cultured. Callus formation occurred from anthers of all cultivars and androgenic embryogenesis was observed from all except P. pyrifolia `Kosui' and P. communis `La France'. Regeneration of adventitious shoots from anther-derived embryos was shown from all apple cultivars and P. pyrifolia `Shinko'. Many of these shoots did not grow or died on half-strength Murashige and Skoog medium (1962) with 4.4 μm BA and 0.5 μm IBA, whereas several shoots of apple `Starking Delicious' grew to plantlets. Chromosome counts of shoot apical cells of four clones derived from embryos of `Starking Delicious' showed that three clones were diploids and one clone comprised diploid and haploid shoots, suggesting that at least one clone originated from a microspore. Chemical names used: 3-indolyl-butyric acid (IBA); N6-benzyladenine (BA).

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Section: Discussionmentioning

confidence: 99%

Plant Regeneration from Anther-derived Embryos of Apple and Pear

Kadota¹,

Han²,

Niimi³

2002

HortSci

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“…When comparing the effect of cotyledon and decapitated embryonic axes explants on percentage of response and number of multiple shoots, the response of embryonic axes was better than cotyledon. Regeneration frequency is generally influenced by the type of explant and embryonic axes reacted better than the cotyledons [49] and whole embryonated cotyledon, sectional embryonated cotyledon, whole de-embryonated cotyledon, sectional de-embryonated cotyledon [50]. This is because of presence of apical and auxiliary meristematic tissue in the embryo axes that are primary sites of shoot regeneration compared to cotyledon which has a lesser amount meristematic tissue that is acquired from the embryonic axes during separation from embryonic axes.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Plant Regeneration of Andrographis Paniculata Nees Using Mature Zygotic Embryonic Explants

Mohammed¹

2017

ARTOAJ

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A rapid method for the large-scale propagation of Andrographis paniculata through in vitro culture of embryonic explants has been developed. Adventitious shoot regeneration from embryonic explants was possible in case of cotyledons and root decapitated embryonic axis among all the tested embryonic explants. High frequency of adventitious shoot formation (?) was observed within 30 days from embryonic explants and shoot induction followed either direct regeneration on MS medium supplemented with BAP or indirect adventitious shoot regeneration in TDZ. BAP was more effective than other cytokinins used for direct adventitious shoot induction from both the embryonic explants of A. panicualata with BAP 1.5mg/L producing 73% shooting and 12.3 shoots per explants while study in cotyledons showed best response in BAP 3.0 mg/L with 52% shooting and 9.3 shoots per explants. While TDZ 1.0mg was effective in indirect shoot regeneration among the different concentrations tested with 26% shoot regeneration from embryonic axis with 3.3 shoots and 76% from cotyledons with 6 shoots per explants. Shoots elongated in MS media with GA 0.5mg/L are effectively rooted in half strength MS media with IBA 0.5mg/L and a high percentage of (82.5%) plants were successfully hardened in soil, sand and vermiculite (1:1:1).

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“…신초 재분화에 있어 서 절편체의 선택은 중요한 요소 중 하나이며, 사과에서 는 원형질체 (Saito and Suzuki. 1999), 생장점으로부터 유도 된 callus (Caboni et al 2000), 자엽과 배축 (Keulemans and de Witte 1994), 엽 절편 (Predieri and Fasolo 1989) 등을 배양재 료로 재분화에 대한 연구가 보고되어 있으며, 배에서는 원형질체 유래 callus (Ochatt and Power 1988), 경정분열조 직 (Lane 1979), 엽 절편 (Chevreau et al 1979) 등을 재료로 하여 재분화에 대한 연구가 보고되어 있다. 배의 경우 주 로 엽 절편체로부터 식물체 재분화에 관한 연구가 보고 되어 있으며 (Leblay et al 1991;Hennayake et al 2003;Lee et al 2002;Chevreau et al 1989), 식물체 정단부 끝 부분에 있는 어린 잎이 아래 부분에 있는 오래된 잎 보다 재분화 에 효율적이라 보고되어 있다 (Chevreau and Leblay 1993 (Kadota et al 2001;Kadota et al 2003), 사과 (Sotiropoulos et al 2006), 살구 (Marino et al 1993), 복숭아 (Ahmad et al 2007)…”

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In vitro shoot regeneration from leaf tissue of "Whangkeumbae" pear(Pyrus pyrifolia Nakai)

Chun¹,

Kim²,

Cho³

et al. 2012

Journal of Plant Biotechnology

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In order to establish an efficient adventitious shoot regeneration conditions from leaf explants for Asian pear 'Whangkeumbae', the effect of concentration and kinds of plant growth regulator and carbon source was investigated. Leaf explants of cultures grown on Murashige and Skoog (MS) medium containing 8 g/L plant agar were used. When the medium contained 0.25 mg/L thidiazuron (TDZ) and 0.3 mg/L indolebutyric acid (IBA), the adventitious shoot regeneration rate (ASRR) was greater as 61.1% than others treated and higher TDZ concentrations (2.5 and 5 mg/L) treatment significantly reduced the ASRR. As the effect of IBA and indoleacetic acid (IAA) concentration on the ASRR, 0.5 mg/L TDZ plus different concentration of IAA exhibited relatively high ASRR and 0.5 mg/L TDZ plus 0.3 mg/L IAA showed the highest ASRR of 76.7%. Also the effect of sucrose and sorbitol as carbon source on regeneration was examined. The highest ASRR and the most shoots per explants averaged 94.4% and 3.49 by treatment of 30 mg/L sorbitol, respectably. Sorbitol is considered better carbon source than sucrose for shoot regeneration of 'Whangkeumbae' pear.

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Plant regeneration from cotyledons and embryonic axes in apple: Sites of reaction and effect of pre-culture in the light

Cited by 5 publications

References 13 publications

Plant Regeneration from Anther-derived Embryos of Apple and Pear

Plant Regeneration from Anther-derived Embryos of Apple and Pear

In Vitro Plant Regeneration of Andrographis Paniculata Nees Using Mature Zygotic Embryonic Explants

In vitro shoot regeneration from leaf tissue of "Whangkeumbae" pear(Pyrus pyrifolia Nakai)

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