Plant regeneration via somatic embryogenesis in chick pea (Cicer arietinum L.)

Sagare, Abhay P.; Suhasini, K.; Krishnamurthy, K. V.

doi:10.1007/bf00232818

Cited by 48 publications

(22 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These findings are in agreement with the earlier reports of Lazzeri et al (1990) in soybean, Eapen and George (1990) in Vigna mungo, Sehgal and Abbas (1994) in Trachyspermum ammi, Girija et al (2000) in Vigna radiata and Ganesh Kumari et al (2008) in Ricinus communis. Our results differ from those of Sagare et al (1993). They reported maximum number of somatic embryos from immature cotyledon derived callus.…”

contrasting

confidence: 85%

See 1 more Smart Citation

Direct somatic embryogenesis and plant regeneration from immature explants of chickpea

Ghanti¹,

Sujata²,

Rao³

et al. 2010

Biologia plant.

View full text Add to dashboard Cite

A protocol for plant regeneration via somatic embryogenesis was developed in two chickpea (Cicer arietinum L.) cultivars ICCV-10 and Annigeri. Somatic embryos were induced from immature cotyledons on Murashige and Skoog's (MS) medium supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), α-naphthaleneacetic acid (NAA) and picloram alone or in combination with 0.5 -2.0 mg dm -3 N 6 -benzylaminopurine (BA) or kinetin (KIN). NAA was better for somatic embryo induction compared to other auxins. The well formed, cotyledonary shaped embryos germinated into plantlets with 36.6 % frequency on MS medium supplemented with 2.0 mg dm -3 BA + 0.5 mg dm -3 abscisic acid (ABA). The frequency of embryogenesis and plantlet regeneration was higher in cv. ICCV-10 as compared to cv. Annigeri. Regenerated plants were transferred to soil (40 % survival) and grown to maturity. Histological studies of explants at various developmental stages of somatic embryogenesis reveled that somatic embryos developed directly from the cotyledon cells and they were single cell origin.

show abstract

contrasting

confidence: 85%

“…L.) is an important grain legume. There are a few reports on its somatic embryogenesis (Rao and Chopra 1991, Barna and Wakhulu 1993, Sagare et al 1993, Kumar et al 1994, 1995, Murthy et al 1996. However, conversion of somatic embryos into plantlets remained inefficient and limited.…”

mentioning

confidence: 98%

Direct somatic embryogenesis and plant regeneration from immature explants of chickpea

Ghanti¹,

Sujata²,

Rao³

et al. 2010

Biologia plant.

View full text Add to dashboard Cite

show abstract

“…Direct shoot organogenesis and establishment of plantlets from different explants of chickpea was reported earlier (Polisetty et al, 1996(Polisetty et al, , 1997Paul et al, 2000;Rizvi and Singh, 2000;Chauhan et al, 2003;Jayanand et al, 2003;Chakraborti et al, 2006). Plantlets were developed through direct somatic embryogenesis and through callus from different explants of chickpea (Barna and Wakhlu, 1993;Sagare et al, 1993;Suhasini et al, 1994;Kumar et al, 1994Kumar et al, , 1995Rizvi and Singh, 2000;Chauhan et al, 2002;Kar et al, 1996Kar et al, , 1997Kiran et al, 2005).…”

Section: Introductionmentioning

confidence: 73%

An optimal protocol for in vitro regeneration, efficient rooting and stable transplantation of chickpea

Anwar

Sharmila

Saradhi

2008

Physiol Mol Biol Plants

View full text Add to dashboard Cite

A rapid, reproducible and efficient regeneration method was developed for chickpea (Cicer arietinum L.) using single cotyledon with half embryonal axis as explants. MS medium supplemented with 4 ìM TDZ, 10 ìM 2-iP and 2 ìM kinetin induced 50-100 adventitious buds/shoots after 14 days of culture and elongated on MS medium supplemented with 5 ìM 2-iP and 2 ìM kinetin. Healthy, strong and 100 % rooting was achieved by exposing cut ends of the shoots to 10 sec pulse treatment with 100 ìmoles/ml IBA followed by their transfer to liquid MS basal medium within 10-14 d. 2-3 cm long shoots were most suitable for rooting. Potting-mixture with good aeration and lesser capacity to retain water was most suitable for achieving successful establishment of chickpea plantlets. Garden soil mixed with sand (gravel) and bio-manure in the ratio of 1:1:1 is most suitable for achieving cent percent transplantation success. Cent percent of plantlets got acclimatized, survived in the pots and showed normal growth, development, flowering followed by podding and seeds setting. Harvesting of seeds was done after the pods were fully matured and dry. In this communication, we have demonstrated for the first time that shoot length, pulse treatment of cut ends of shoots with 100 ìmoles/ml IBA and aeration of potting mixture are key factors for rapid micro-propagation and successful establishment of chickpea.

show abstract

“…Despite in vitro regeneration from shoot meristem [2,3], immature cotyledons [4] and through embryo genesis from immature cotyledons [5], leaflet callus [6][7][8], influence of genotype regeneration capacity poorly studied. Present paper describes the complete regeneration in chickpea varieties.…”

Section: Introductionmentioning

confidence: 99%

De Novo Regeneration in Chickpea Genotypes (Cicer Arietinum L.)

JAMBHALE¹

2017

RIB

View full text Add to dashboard Cite

Direct regeneration from mature embryo axes was achieved without intervening of callus phase in four chickpea varieties on the Media MS and B5 supplemented with combination of BAP, NAA and Kinetin. Hundred percent regeneration capacity was exhibited by commercially grown Vijay and Vishal varieties. There was considerable variation in umber of multiple shoot production by different varieties. Profuse rooting was obtained on the medium containing 0.5 and 1.0 mg/l IBA. This protocol is optimized for complete plant regeneration of chickpea for genetic transformation.

show abstract

Plant regeneration via somatic embryogenesis in chick pea (Cicer arietinum L.)

Cited by 48 publications

References 8 publications

Direct somatic embryogenesis and plant regeneration from immature explants of chickpea

Direct somatic embryogenesis and plant regeneration from immature explants of chickpea

An optimal protocol for in vitro regeneration, efficient rooting and stable transplantation of chickpea

De Novo Regeneration in Chickpea Genotypes (Cicer Arietinum L.)

Contact Info

Product

Resources

About