Plantlet Formation by Anther Culture of Citrus aurantium L.

Hidaka, Tetsushi; Yamada, Yoshio; Shichijo, T.

doi:10.1270/jsbbs1951.32.247

Cited by 31 publications

(20 citation statements)

References 10 publications

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“…In the previous studies, plantlets were obtained from anthers of trifoliate orange (5) and sour orange (6), but the two kinds of oranges required different concentrations of growth regulators to obtain pollen plants. The media containing 0.2 or 2.0 mg/l kinetin and 0.2 mg/l IAA were effective in trifoliate orange, while relatively low concentrations of kinetin were required by sour orange.…”

Section: Discussionmentioning

confidence: 99%

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Induction of Plantlets from Anthers of^|^lsquo;Trovita^|^rsquo;Orange

Hidaka¹

1984

Engei Gakkai zasshi

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SummaryAnther culture of `Trovita' orange, a cultivar of sweet orange (Citrus sinensis Osbeck), was attempted.Anthers with microspores at uninucleate stage were cultured on the Murashige and Skoog's medium(9) supplemented with or without indole-3-acetic acid (IAA) and kinetin at 28°C in darkness.Embryoids appeared directly from within anthers after about 10 weeks of culture.The media containing kinetin and IAA both at 0.002 or 0.02 mg/1 were effective in embryoid formation.In addition, 0.2 or 2.0 mg/1 kinetin and 0.2 mg/1 IAA seemed to be also favorable when combined with 0.002 or 0.02 mg/1 IAA and 0.002 or 0.02 mg/1 kinetin, respectively.Calli were formed well on the media with kinetin at 2.0 mg/1 followed by those with IAA at 2.0 mg/l.After incubation in light with a 16-hour photoperiod, some embryoids differentiated shoots.Induction of roots was stimulated by transfer of embryoids from the embryoid induction media containing growth regulators to a medium without them.Some root tip cells of differentiated plantlets had a diploid chromosome number of 18.

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Section: Discussionmentioning

confidence: 99%

“…(4) (6). However, there is no report on the induction of plantlets, from anthers of sweet orange, one of the principal species in the citrus industry of the world.…”

Section: Introductionmentioning

confidence: 99%

Induction of Plantlets from Anthers of^|^lsquo;Trovita^|^rsquo;Orange

Hidaka¹

1984

Engei Gakkai zasshi

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“…In Citrus, the production of embryogenic callus lines was reported from excised nucelli (Rangan et al, 1968), abortive ovules (Bitters et al,1970), unfertilized ovules (Button and Bornman, 1971), undeveloped ovules (Starrantino and Russo, 1980), juice vesicles (Nito and Iwamasa, 1990) , anthers (Hidaka et al ., 1981), styles and stigmas (Carimi et al, 1995) as well as from leaves, epicotyls, cotyledons and root segments (Gill et al, 1995 …”

Section: Tissue Culture (Micropropagation) Is a Powerful Tools Fomentioning

confidence: 99%

The utilities of Citrus tissue culture

Chamandoosti¹

2017

IJOEAR

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“…In particular, anther culture has produced somatic regenerants in C. aurantium (Hidaka et al 1981;Germanà 2005); C. sinensis (Hidaka 1984); C. aurantifolia (Chaturvedi & Sharma 1985); C. madurensis (Ling et al 1988); C. reticulata (Germanà et al 1994;Germanà 2005); P. trifoliata, and the hybrid 14 of C. ichangensis £ C. reticulata (Deng et al 1992). In these cases, anther culture can be regarded as an additional method for achieving somatic embryogenesis.…”

Section: Characterization Of Regenerants: Ploidy Analysis and Analysimentioning

confidence: 99%

Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes

Cardoso

Abdelgalel

Chiancone

et al. 2015

Plant Biosystems - An International Journal Dealing with all As

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In vitro tissue culture represents a useful technique for advancing Citrus breeding and propagation. Among in vitro regeneration systems, anther culture is commonly used to produce haploids and doubled haploids for a fast-track producing homozygous lines, in comparison with the traditional self-pollination approach, which involves several generations of selfing. In addition, anthers culture can produce somatic embryos that can also be used for clonal propagation. In this study, two thermal shocks were applied to the anthers of six Citrus genotypes (two clementine and four sweet oranges), just after they were put in culture. The response obtained was different depending on the genotype: both clementines, namely Hernandina and Corsica, produced homozygous and triploid regenerants (microspore-derived embryos), whereas all of the analyzed regenerants from sweet oranges, three cultivars of Tarocco and Moro, produced heterozygous and diploid regenerants similar to the parental genotypes (somatic embryos).

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Plantlet Formation by Anther Culture of Citrus aurantium L.

Cited by 31 publications

References 10 publications

Induction of Plantlets from Anthers of^|^lsquo;Trovita^|^rsquo;Orange

Induction of Plantlets from Anthers of^|^lsquo;Trovita^|^rsquo;Orange

The utilities of Citrus tissue culture

Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes

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