Plasma circular RNA profiling of patients with gastric cancer and their droplet digital RT-PCR detection

Li, Tianwen; Shao, Yongfu; Fu, Liyun; Xie, Youhua; Zhu, Linwen; Sun, Weiliang; Yu, Ran; Xiao, Bingxiu; Guo, Junming

doi:10.1007/s00109-017-1600-y

Cited by 221 publications

(174 citation statements)

References 33 publications

Supporting

Mentioning

171

Contrasting

Order By: Relevance

“…Among the included publications, a total of 43 articles on 64 studies were analyzed involving 10,123 subjects in Table . Of the 64 studies, five studies focused on colorectal cancer (Ji et al, ; Wang, Li, et al, ; Wang et al, ; Zhang, Yang, et al, ; Zhuo, Lin, Chen, Huang, & Hu, ), 10 on breast cancer (Lu et al, ; Yin et al, ), 22 on gastric cancer (Chen, Li, Zhao, Xiao, & Guo, ; Huang, He, Liang, Huang, & Zhu, ; Li, et al, , ; Li, Shao, et al, ; Li, Song, et al, ; Lu et al, ; Lu, Shao, Ye, Xiao, & Guo, ; Shao, Chen, et al, ; Shao, Li, et al, ; Shao, Yang, et al, ; Sun, Tang, et al, ; Tian, Chen, Li, & Xiao, ; Wei et al, ; Xie et al, ; Zhao, Chen, Li, Xiao, & Zhang, ), four on lung cancer (Li, Wang, Chen, Chen, & Jin, ; Zhu et al, ; Zong et al, ), 16 on hepatocellular carcinoma (Chen, Zhang, Lin, Song, & Wang, ; Fu et al, , ; Li et al, ; Qin et al, ; Shang et al, ; Yao et al, ; Yao et al, ; Zhang, Zhang, Lin, & Wang, ; Zhang, Zhou, et al, ; Zhang, Xu, et al, ) and seven on other cancers (Fan, Cao, Liu, Zhang, & Li, ; Lan et al, ; Li et al, ; Sun, Li, et al, ; Teng et al, ; Wang, Xue, et al, ; Yang et al, ). Moreover, three articles with 13 studies explored the diagnostic value of the combined circRNAs (Chen, Li, Zhao, et al, ; Fan et al, ; Li, Shao, et al, ) in Table .…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, several studies provided that single circRNAs in combination with other plasma protein biomarkers such as CEA, CA‐15.3, CA‐125 and AFP possess better diagnostic performance (Huang et al, ; Prefumo, ; Yang et al, ; Yin et al, ; Zhang, Yang, et al, ). Additionally, a single circRNA combined with other circRNAs obtained also had better diagnostic accuracy (Li, Chen, et al, ; Li, Shao, et al, ; Ren & Xin, ). For example, Li et al identified that has_circ_0000096 and hsa_circ_002059 have moderate diagnosis value for gastric cancer, with an AUC of 0.82 and 0.73 respectively, while the AUC value of hsa_circ_0000096 combined with hsa_circ_002509 increased to 0.91 (Li, et al, , ).…”

Section: Discussionmentioning

confidence: 99%

“…For example, Li et al identified that has_circ_0000096 and hsa_circ_002059 have moderate diagnosis value for gastric cancer, with an AUC of 0.82 and 0.73 respectively, while the AUC value of hsa_circ_0000096 combined with hsa_circ_002509 increased to 0.91 (Li, et al, , ). Besides, when hsa_circ_0001017 in tissue combined with plasma, diagnostic accuracy was greatly improved for gastric cancer (AUC: 0.952) (Li, Shao, et al, ). In addition, the combination of hsa_circ_0001017 and hsa_circ_0061276 in tissue and plasma gained better efficacy for gastric cancer (AUC: 0.966).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Diagnostic performance of circular RNAs in human cancers: A systematic review and meta‐analysis

et al. 2019

Molec Gen & Gen Med

View full text Add to dashboard Cite

Background Recently, accumulating evidence have revealed that circular RNA (circRNA) was deregulated in multiple types of cancer, suggesting that circRNA might serve as a novel candidate biomarker of cancer diagnosis. However, inconsistent results have become an obstacle in applying circRNAs to clinical practice. The aim of this study is to evaluate diagnostic value of circRNAs among cancers. Methods A literature search was systematically performed among PubMed, Sciencedirect, Cochrane Library, Web of Science, Wanfang, and Chinese National Knowledge Infrastructure databases up to February 15, 2019. The pooled sensitivity (SEN), specificity (SPE), positive likelihood ratios, negative likelihood ratios, diagnostic odds ratio, and area under the SROC curve (AUC) were applied to evaluate diagnostic performance of circRNAs. Results In total, the study included 64 studies with single circRNA and 13 studies with combined circRNAs. Overall, the study presented that a single circRNA had moderate diagnostic value, with a SEN of 0.75, a SPE of 0.76, and an AUC of 0.82. The plasma circRNAs had higher diagnostic accuracy than tissue (AUC: 0.87, 95% confidence interval [CI]: 0.83–0.89 for plasma/serum subgroup; AUC: 0.79, 95% CI: 0.75–0.82 for tissue subgroup). Furthermore, the combined circRNAs had good diagnostic efficacy for GC, with a SEN of 0.89, a SPE of 0.94, and an AUC of 0.97, respectively. Conclusion This study confirmed that circRNAs may be candidate biomarkers for cancer diagnosis. In particular, diagnosis of combined circRNAs will be a new alternative applied to clinical research and practice for cancer.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Diagnostic performance of circular RNAs in human cancers: A systematic review and meta‐analysis

et al. 2019

Molec Gen & Gen Med

View full text Add to dashboard Cite

show abstract

“…The qRT‐PCR products were confirmed by Sanger sequencing, which was completed by Geneseed. The relative quantification levels of hsa_circ_0067582 were calculated using the ΔC q (quantification cycle) method . A higher ΔC q value indicated a lower expression level .…”

Section: Methodsmentioning

confidence: 99%

Reduced expression of circRNA hsa_circ_0067582 in human gastric cancer and its potential diagnostic values

Ding

Yang

et al. 2019

Clinical Laboratory Analysis

Self Cite

View full text Add to dashboard Cite

Background Gastric cancer (GC) is one of the global mortality diseases and has a poor prognosis due to the lack of ideal tumor biomarkers. Circular RNAs (circRNAs) are an abundant kind of endogenous RNAs that recently are found play a crucial role in the cancer occurrence and development. Nevertheless, little is known with regard to the diagnostic values of these circRNAs for GC. In this article of research, we investigated the role of hsa_circ_0067582 in clinical diagnosis of GC. Materials and Methods We used divergent primers, and the expression levels of hsa_circ_0067582 in 93 fresh GC tissues and paired adjacent normal tissues from surgical patients were detected using quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). Then, a receiver operating characteristic (ROC) curve was established to assess the diagnostic significance of hsa_circ_0067582. The relationship between expression of hsa_circ_0067582 and clinicopathological factors of patients was made further explored. Results Hsa_circ_0067582 levels were significantly decreased in GC tissues contrasted with adjacent normal tissues (n = 93, P < .001). After that, we discovered that it was evidently downregulated in 81.7% (76/93) GC tissues. The area under the ROC curve (AUC) of hsa_circ_0067582 was up to 0.6937, the sensitivity was 66.67%, and the specificity was 61.29%. Moreover, the hsa_circ_0067582 levels were obviously associated with tumor diameter (P = .002) and carbohydrate antigen 19‐9 (CA19‐9, P = .01). Meanwhile, after operation, low‐level group of hsa_circ_0067582 of GC patients was associated with better prognosis. Conclusion Our data imply that hsa_circ_000067582 may be a potential biomarker for GC diagnosis and prognosis evaluation.

show abstract

“…A single peak in the melting curve indicated the specificity of qRT‐PCR results. The data were analyzed using the Δ C q method . And the expression of circRNAs in cells was analyzed by the 2 −ΔΔ C q method.…”

Section: Methodsmentioning

confidence: 99%

Circular RNA 0068669 as a new biomarker for hepatocellular carcinoma metastasis

Yao

Chen

Shao

et al. 2018

Clinical Laboratory Analysis

Self Cite

View full text Add to dashboard Cite

show abstract

Plasma circular RNA profiling of patients with gastric cancer and their droplet digital RT-PCR detection

Cited by 221 publications

References 33 publications

Diagnostic performance of circular RNAs in human cancers: A systematic review and meta‐analysis

Diagnostic performance of circular RNAs in human cancers: A systematic review and meta‐analysis

Reduced expression of circRNA hsa_circ_0067582 in human gastric cancer and its potential diagnostic values

Circular RNA 0068669 as a new biomarker for hepatocellular carcinoma metastasis

Contact Info

Product

Resources

About