Plasma Concentration of Insulin-like Growth Factor I (IGF-I) in Growing Ardenner Horses Suffering from Juvenile Digital Degenerative Osteoarthropathy

Lejeune, Jean-Philippe; Franck, Thierry; Gangl, Monika; Schneider, Nicole; Michaux, Charles; Deby‐Dupont, G.; Serteyn, Didier

doi:10.1007/s11259-006-3385-2

Cited by 12 publications

(20 citation statements)

References 39 publications

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“…In summary, it has been confirmed that IGF-1 concentrations are reduced in dogs with liver diseases (Neumann et al, 2007), congenital portosystemic shunts (Maxwell et al, 2000) and mitral regurgitation (Pedersen et al, 2005). In horses, decreased circulating IGF-1 concentrations have been found in osteochondrosis (Sloet van Oldruitenborgh-Oosterbaan et al, 1999), juvenile digital osteoarthropathy (Lejeune et al, 2007) and in several joint diseases (Verwilghen et al, 2009). In contrast, some disorders associated with insulin resistance, such as pars intermedia pituitary dysfunction, equine metabolic syndrome, pasture-associated laminitis and hyperlipemia might be related to high serum IGF-1 concentrations (Munoz et al, 2010b).…”

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confidence: 79%

A study of serum insulin-like growth factor type 1 (IGF-1) concentrations in resting untrained Andalusian horses: influence of age and gender

Muñoz¹,

Trigo²,

Riber³

et al. 2011

Vet. Med.

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Growth rate, tissue repair and reproductive functions are mediated by the somatotrophic axis, the growth hormone (GH) being one of its main components. GH is released in a pulsatile manner and a single measurement does not provide accurate information on the activity of the somatotrophic axis. The actions of GH on tissues are mediated by insulin-like growth factor type 1 (IGF-1), mainly released by the liver, and thus, the measurement of IGF-1 could be considered a good indicator of the activity of GH and the somatotrophic axis. Serum IGF-1 concentrations are relatively stable due to its long biological half-life without obvious diurnal rhythm. Additionally, many diseases significantly alter circulating IGF-1 concentrations, leading to potential diagnostic and prognostic uses in veterinary medicine. However, serum IGF-1 concentrations are affected by many factors, such as breed, age and sex. The present study analyzes the influence of these factors on serum IGF-1 concentrations in a population of 255 Andalusian horses (141 females and 114 males), divided into age groups: 1-2, 2-3, 3-4, 4-5, 5-6 and 6-12 months and 1-2, 2-4, 4-6, 6-10 and 10-14 years. The animals belonged to six different farms located in the same geographic location and were subjected to similar feeding and management protocols. Two measurements of body size were made: height at the withers (HW) and diameter of the thorax (DTx). Blood samples were taken always in the morning, in the month of July and serum IGF-1 concentrations were measured with a sandwich ELISA after dissociation of IGF-1 from its binding proteins. It was found that age and sex significantly influenced serum IGF-1 concentrations, whereas the effects of the farm and the time of blood withdrawal were not significant. Mean serum concentrations for both males and females respectively were: 246.3 and 231.0 (1-2 months), 201.9 and 194.7 (2-3 months), 174.2 and 170.4 (3-4 months), 161.7 and 155.4 (4-5 months), 166.1 and 136.9 (5-6 months), 127.2-114.5 (6-12 months), 103.3 and 89.01 (1-2 years), 104.3 and 73.41 (2-4 years), 105.4 and 64.40 (4-6 years), 53.29 and 68.27 (6-10 years) and 59.56 and 65.53 ng/ml (10-14 years). A progressive decrease in serum IGF-1 concentrations with increased age was found for both sexes. Males aged between five and 12 months and between two and six years had significantly higher serum IGF-1 concentrations than females of the same age. Coefficients of correlation between the indicators of body size (HW and DTx) and IGF-1 were -0.800 and -0.690 for the whole population of Andalusian horses, -0.860 and -0.750 for the males and -0.740 and -630 for the females. It is concluded that serum IGF-1 concentrations in Andalusian horses are reduced with ageing, male horses of determined age groups had higher IGF-1 than the females and there are negative correlations between body size and IGF-1 concentrations. The knowledge of the normal serum IGF-1 concentrations will help us to understand the role of the somatotrophic axis in several diseases and physiologic...

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confidence: 79%

A study of serum insulin-like growth factor type 1 (IGF-1) concentrations in resting untrained Andalusian horses: influence of age and gender

Muñoz¹,

Trigo²,

Riber³

et al. 2011

Vet. Med.

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“…Dentre eles o fator de crescimento tipo insulina 1 (IGF-I) tem mostrado promover o crescimento e a diferenciação de condrócitos (Henson et al 1997), além de estimula-los a sintetizar constituintes da matriz cartilagínea como colágeno, proteoglicanos e ácido hialurônico (Verwilghen et al 2009b), induzindo o reparo cartilagíneo (Fortier et al 2001). Também foi relatado que o IGF-1 protege os condró-citos da apoptose induzida por diferentes estímulos, inibe os efeitos catabólicos da interleucina-1 sobre a síntese de proteoglicanos, e suprime a degradação dos componentes da matriz cartilagínea reduzindo a expressão e atividade de metaloproteinases 1 e 8 (Lejeune et al 2007).…”

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“…Logo, qualquer distúrbio nos elementos acima pode desregular os mecanismos envolvidos no controle local da integridade articular (Lejeune et al 2007).…”

Section: Introductionunclassified

“…Aparentemente, potros acometidos por osteocondrose apresentam uma atividade IGF-I significativamente menor do que potros sadios (Sloet van Oldruitenborgh-Oosterbaan et al 1999, Lejeune et al 2007). Também foi relatado que a deficiência de energia e/ou proteína, ou principalmente o desequilíbrio entre energia e proteína na dieta de potros entre 15 e 28 meses reduz a produção de IGF-1 (Lejeune et al 2007).…”

Section: Introductionunclassified

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Identificação dos níveis séricos do fator de crescimento tipo insulina 1 em potros com osteocondrose

Baccarin¹,

Pereira²,

Roncati³

et al. 2011

Pesq. Vet. Bras.

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A osteocondrose é uma das doenças ortopédicas do desenvolvimento mais comuns em cavalos, e deve ser encarada como uma desordem multifatorial. Caracteriza-se por ser uma doença dinâmica, onde o processo de reparo inicia-se quase que imediatamente após a formação da lesão osteocondral. A intensidade e eficácia deste reparo são fortemente relacionados à idade, e estão associados a reposição dos componentes da matriz cartilagínea. Uma vez que o fator de crescimento tipo insulina-1 (IGF-1) age regulando o crescimento da cartilagem articular, este estudo objetivou descrever os níveis séricos de IGF-I, insulina e glicose em potros Puro Sangue Lusitano hígidos ou com osteocondrose, desde o nascimento até 18 meses de idade. Dos potros acompanhados, 76,08% apresentaram sinais radiográficos de osteocondrose com um mês de idade, porém somente 16,2% mantiveram as lesões aos 18 meses. As concentrações de IGF-1 alcançaram picos entre 14 e 16 meses, equivalente ao início da puberdade. As concentrações de IGF-1 nos potros com osteocondrose foram menores que potros sadios, aos 2 e 5 meses (P<0,05), e os de insulina aos 2 meses (P<0,05). Em criatórios com alta ocorrência de osteocondrose recomenda-se que as mensurações dos níveis séricos de IGF-1 e insulina ocorram durante os primeiros 6 meses de idade, para que haja tempo hábil de se estabelecer medidas profiláticas e de tratamento.

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“…Investigators have suggested that IGF-1 plays a critical role in the expression and synthesis of collagen type II as well as protecting chondrocytes from apoptosis. Therefore, changes in concentrations of IGF-1 could modulate the biological mechanisms that regulate joint and bone development (Lejeune et al, 2007). Serum IGF-1 concentrations appear to peak in horses at 10 months of age correlating with the beginning of reproductive maturity and then begin to decrease, reaching a steady adult concentration at approximately 450 days old (Fortier et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Comparison and validation of ELISA assays for plasma insulin-like growth factor-1 in the horse

Baskerville¹,

Bamford²,

Harris³

et al. 2017

Open Vet J.

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Insulin-like growth factor-1 (IGF-1) plays several important physiological roles, and IGF-related pathways have been implicated in developmental osteochondral disease and endocrinopathic laminitis. This factor is also a downstream marker of growth hormone activity and its peptide mimetics. Unfortunately, previously used assays for measuring equine IGF-1 (radioimmunoassays and ELISAs) are no longer commercially available, and many of the kits on the market give poor results when used on horse samples. The aim of the present study was to compare three different ELISA assays (two human and one horse-specific). Plasma samples from six Standardbreds, six ponies and six Andalusians were used. The human IGF-1 ELISA kit from Immunodiagnostic Systems (IDS) proved to be the most accurate and precise of the three kits; the other two assays gave apparently much lower concentrations, with poor recovery of spiked recombinant human IGF-1 and unacceptably poor intra-assay coefficients of variation (CV). The IDS assay gave an intra-assay CV of 3.59 % and inter-assay CV of 7.31%. Mean percentage recovery of spiked IGF-1 was 88.82%, and linearity and dilutional parallelism were satisfied. The IGF-1 plasma concentrations were 123.21 ±8.24 ng/mL for Standardbreds, 124.95 ±3.69 ng/mL for Andalusians and 174.26 ±1.94 ng/mL for ponies. Therefore of the three assays assessed, the IGF-1 ELISA manufactured by IDS was the most suitable for use with equine plasma samples and may have many useful applications in several different research areas. However, caution should be used when comparing equine studies where different analytical techniques and assays may have been used to measure this growth factor.

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Plasma Concentration of Insulin-like Growth Factor I (IGF-I) in Growing Ardenner Horses Suffering from Juvenile Digital Degenerative Osteoarthropathy

Cited by 12 publications

References 39 publications

A study of serum insulin-like growth factor type 1 (IGF-1) concentrations in resting untrained Andalusian horses: influence of age and gender

A study of serum insulin-like growth factor type 1 (IGF-1) concentrations in resting untrained Andalusian horses: influence of age and gender

Identificação dos níveis séricos do fator de crescimento tipo insulina 1 em potros com osteocondrose

Comparison and validation of ELISA assays for plasma insulin-like growth factor-1 in the horse

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