Plasma levels of free fatty acids, lipoprotein lipase, and postheparin esterase in pregnancy

Fabian, Eric; Stork, A; Kucerová, L; Sponarová, J

doi:10.1016/s0002-9378(15)33746-7

Cited by 23 publications

(5 citation statements)

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“…Thus, in starvation a well as in hyperthyroidism (1 1 ) a diminished response in PHLA is not accompanied by an accumulation of endogenous triglycerides. These situations contrast with those of pregnancy (12,13), hypothyroidism ( 14) , and non-nephrotic uremia ( 15) whfere diminished PHLA is accompanied by increased levels of triglyceride. The failure of triglycerides to increase during starvation may simply reflect diminished hepatic production, but may also suggest that enzymes other than adipose tissue lipoprotein lipase play a role in triglyceride clearing.…”

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confidence: 90%

Post-Heparin Lipolytic and Monoglyceridase Activities in Fasted Man

Arons

Schreibman

Arky

1971

Experimental Biology and Medicine

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Animal studies reveal diminished adipose tissue "clearing factor" lipase after starvation (1, 2 ) ; however, no measurements of heparin-releasable lipases have been made in fasted humans. Since plasma post-heparin lipolytic activity is used clinically to assess defects in the removal of triglycerides ( 3 ) , it seemed essential to recognize the physiologic factors that affect such activity. Accordingly, we measured plasma post-heparin activity against a soy bean oil emulsion and micellar monoolein in individuals fasted for 10 days, and then after refeeding. Prolonged fasting in man causes a significant decrease in postheparin lipolytic and monoglyceridase activity. Plasma triglyceride fell in the face of these declines in post-heparin lipolytic activi ty.Methods and Materials. Six euthyroid, obese volunteers were fasted on a metabolic ward after a t least 3 days of an equilibration diet consisting of 40% mixed carbohydrate, 20% protein, and 40% fat (60% unsaturated, 40% saturated; approximately 700 mg cholesterol). Patients were studied before fast, and on days 3, 7, and 10 of total caloric restriction during which daily intake consisted of 50 mEq NaCl, 87 mEq KCl, and 2008 ml of water. Patients were restudied between 8 and 9 a.m. after 1 day of refeeding with 1500 calories. Nonobese euthyroid controls ( N = 15) recruited among hospital personnel and convalescing patients were studied after an overnight fast. All subjects were free of renal, hepatic, or lipid disorders, and none was pregnant or taking oral contraceptives. The nature of the studies was fully explained and signed consent obtained.On the morning of study, subjects were given 0.1 mg/kg of sodium heparin (Lipo-Hepin, Riker) intravenously and four venous plasma samples were obtained at 2-min intervals between 6 and 12 min after injection. Samples were centrifuged at 4O, the postheparin plasma pooled and stored at -20'. Post-heparin lipolytic activity (PHLA) was determined by a modified Frederickson method ( 3 ) using a soy bean oil emulsion (Intralipid, Vitrum, Stockholm) as described previously (4). Post-heparin monoglyceridase activity (PHMA) was determined using a mono-olein micelle solution. Glyceryl monooleate, 90% ( Calbiochem2) was sonicated (Sonifier Cell Disrupter, model W140D, U1trasonics, Inc.) in a solution of 4% taurodeoxycholic acid (sodium salt, Sigma) in KRP buffer at ph 7.4 to give a final concentration of 3 % . Post-heparin plasma (0.4 ml) was incubated with 0.6 ml of the substrate at 37" for 90 min. Free fatty acids were measured by the method of Dole ( 5 ) . Pre-heparin plasma triglycerides were measured as described previously (4).Partial thromboplastin times (6) were performed on pre-and 10-min post-heparin (0.1 mg/kg) samples after 9 days of starvation to rule out heparin antagonism in the evaluation of post-heparin lipases. Statistical analyses were made on the Mathatron 4280 computer.Results. I n 15 nonobese volunteers, the mean t SEM PHLA was 6.4 t 0.4 p&I FFA/ml/hr and PHMA was 8.4 t 0.4 pM 2 Thin-layer chromatography (methan...

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Post-Heparin Lipolytic and Monoglyceridase Activities in Fasted Man

Arons

Schreibman

Arky

1971

Experimental Biology and Medicine

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“…Ouring pregnancy, especially in the third trimester, LPL and PHE activities fall significantly and after delivery they return to the initial values (Sandhofer et al 1962, Fabian et al 1968. When postmenopausal women are given estrogens, LPL and PHE activities are significantly decreased (Fabian et al 1967a, Fabian andKobilkova 1969).…”

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confidence: 99%

Lipoprotein Lipase and Postheparin Esterase Activity After the Administration of Testosterone to Healthy Men and Women and to Patients with Cirrhosis of the Liver

Fabian¹,

Kobilková²,

Stork³

et al. 1972

Horm Metab Res

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Lipoprotein lipase and post heparin esterase activity was determined in groups of healthy men and women and in a group of men with cirrhosis of the liver before the administration of 125 mg testosterone propionate (5 x 25 mg intramuscularly) on the day after the completion of treatment and six weeks later. Lipoprotein lipase and post heparin esterase activity increased significantly in all groups under the influence of testosterone with the exception of lipoprotein lipase in cirrhotics where the increase in activity was not significant. Postheparin esterase reacted to the hormone with a much greater increase in activity which lasted longer than in the case of lipoprotein lipase. The greatest increase in postheparin esterase was recorded in cirrhotics where it amounted to 300% of the initial value. Testosterone acted on these two lipolytic enzymes opposite to the effect of estrogens. This effect is attributed to its anabolie action and to the altered metabolism of this hormone.

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“…Da der Anstieg der unveresterten Fettsäuren nach Heparinverabreichung vornehmlich von der Aktivierung der Lipoprotein-Lipase abhängt (12,15), ist es möglich, sich mittels der Bromsulphthaleinmethode über die Aktivität dieses Enzyms in vivo zu informieren. Im Einklang mit unseren Befunden beobachtete THER-KELSEN (16), daß die intravenöse Verabreichung von Heparin bei Hunden Veränderungen in der Spektralabsorption des Bromsulphthalein-Serum-Gemisches hervorruft, die er der Bindung von freigewordenen unveresterten Fettsäuren an Albumin zuschreibt, wenn er auch deren Konzentration im Plasma nicht zugleich bestimmte.…”

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“…Standardlosung von Bromnach intravenöser Verabreichung von Heparin der Fall sulphthalein ^ ßoratpuffer mit pH 9,1. Die Extinktion der Bromist (12,13) …”

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Bestimmung der Aktivität der Lipoprotein-Lipase im Plasma nach Heparinverabreichung mit Hilfe der Bromsulphthaleinmethode

Kucerová¹,

Hoenig²,

Fabian³

et al. 1966

Clinical Chemistry and Laboratory Medicine

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Bei 23 gesunden Personen wurden im Nüchternplasma und im Plasma, das 10 Min. nach intravenöser Verabreichung von 50 mg Heparin entnommen wurde, die Konzentration der unveresterten Fettsäuren und die Albuminämie bestimmt, letztere sowohl elektrophoretisch, als auch mit der Bromsulphthaleinmethode. Wir konnten bestätigen, daß nach Heparinverabreichung die Konzentration der unveresterten Fettsäuren ansteigt. Diese Fettsäuren binden sich an Albumin und bewirken so eine herabgesetzte Bindungsfähigkeit des Plasmaeiweißes für Bromsulphthalein. Die mit der Bromsulphthaleinmethode ermittelten Albuminämiewerte sind daher scheinbar herabgesetzt. Daraus geht hervor, daß die Bromsulphthaleinmethode bei erhöhtem Plasmaspiegel der unveresterten Fettsäuren zur Bestimmung der Albuminämie nicht verwendet werden kann. Der Anstieg der Konzentration der unveresterten Fettsäuren nach Heparinverabreichung ist dem Anstieg der Extinktion einer Standardlösung von Bromsulphthalein und Plasma nach Heparinverabreichung direkt proportional. Aus der Extinktionsänderung der Bromsulphthaleinlösung kann der Anstieg der unveresterten Fettsäuren im Plasma mit einem Fehler von ±16,2% berechnet werden. Die Bromsulphthaleinmethode gestattet also, die Menge der bei der Klärungsreaktion freigewordenen unveresterten Fettsäuren zu bestimmen und indirekt so die Aktivität der Lipoprotein-Lipase in vivo festzustellen.23 Healthy persons were used to determine the concentrations of unesterified fatty acids and albuminaemia in resting plasma and in plasma taken 10 min. after the intravenous injection of 50 mg. of heparin. Albuminaemia was measured both electrophoretically and by the bromosulphalein method. We showed that the concentration of unesterified fatty acids increased after the administration of heparin. These fatty acids became bound to albumin and thereby rendered the plasma protein less reactive with bromosulphalein. Thus the albumin value by the bromosulphalein method was apparently low. The bromosulphalein method for albuminaemia cannot therefore be used when the level of unesterified plasma fatty acids is increased. After the administration of heparin, the increase in the concentration of free fatty acids is directly proportional to the increase in the extinction of a standard solution of bromosulphalein and plasma. The increase in the level of unesterified plasma fatty acids can be determined from the change in extinction of the bromosulphalein solution with an error of ±16.2%. Therefore, the bromosulphalein method permits the measurement of the unesterified fatty acids in the clearing reaction and, indirectly, the activity of the lipoprotein lipase in vivo.Unveresterte Fettsäuren (UFS) sind im Blutplasma an Verminderung zur Folge hätte. Bei steigender KonzenEiweiß gebunden, vorwiegend an Albumin (l-4). An tration der unveresterten Fettsäuren, die sich an AlbuPlasmaalbumin binden sich außerdem noch andere min binden, würde nämlich der Albuminanteil, der Stoffe (5, 6), und zwar sowohl endogene (Bilirubin, fähig ist Bromsulphthalein zu binden, abs...

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Plasma levels of free fatty acids, lipoprotein lipase, and postheparin esterase in pregnancy

Cited by 23 publications

References 15 publications

Post-Heparin Lipolytic and Monoglyceridase Activities in Fasted Man

Post-Heparin Lipolytic and Monoglyceridase Activities in Fasted Man

Lipoprotein Lipase and Postheparin Esterase Activity After the Administration of Testosterone to Healthy Men and Women and to Patients with Cirrhosis of the Liver

Bestimmung der Aktivität der Lipoprotein-Lipase im Plasma nach Heparinverabreichung mit Hilfe der Bromsulphthaleinmethode

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