Platelet Activation in Stored Platelet Concentrates: Comparision of Two Methods Preparation

Ali, Soleimany Ferizhandy

doi:10.4172/2155-9864.1000107

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…For transfusion purposes, blood is usually collected in bags containing citrate phosphate dextrose adenine (CPDA-1) solution, as anticoagulant [ 22 , 23 ], from which a platelet concentrate is obtained by double centrifugation of the whole blood or apheresis. Platelets concentrates obtained by such methodologies may also be used for promoting tissue repair [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Platelet‐Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro

Amaral

Silva²,

Haddad³

et al. 2016

Stem Cells International

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There are promising results in the use of platelet-rich plasma (PRP) for musculoskeletal tissue repair. However, the variability in the methodology for its obtaining may cause different and opposing findings in the literature. Particularly, the choice of the anticoagulant is the first definition to be made. In this work, blood was collected with sodium citrate (SC), ethylenediaminetetraacetic acid (EDTA), or anticoagulant citrate dextrose (ACD) solution A, as anticoagulants, prior to PRP obtaining. Hematological analysis and growth factors release quantification were performed, and the effects on mesenchymal stromal cell (MSC) culture, such as cytotoxicity and cell proliferation (evaluated by MTT method) and gene expression, were evaluated. The use of EDTA resulted in higher platelet yield in whole blood; however, it induced an increase in the mean platelet volume (MPV) following the blood centrifugation steps for PRP obtaining. The use of SC and ACD resulted in higher induction of MSC proliferation. On the other hand, PRP obtained in SC presented the higher platelet recovery after the blood first centrifugation step and a minimal change in MSC gene expression. Therefore, we suggest the use of SC as the anticoagulant for PRP obtaining.

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Section: Introductionmentioning

confidence: 99%

Platelet‐Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro

Amaral

Silva²,

Haddad³

et al. 2016

Stem Cells International

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show abstract

The Influence of Environmental and Genetic Factors on Various Disorders and Diseases

Tripathy¹,

Tushar²,

Sudharani³

2011

J Genet Syndr Gene Ther

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Both genetic and environmental factors have been implicated in the mechanism of various disorders. Epidemiological data have shown that environmental factors can also cause and/or exacerbate the pathogenesis of these diseases. However in this article, we provide a summary of evidence for environmental and genetic factors influencing the diseases. The review also incorporates the major findings categorizing the common diseases on the basis of genetic profiles and ethnic information and in establishing personalized disease diagnosis, drug responses and treatment modalities based on the genetic determinants. These studies provide important insights into the interplay between environmental and genetic factors leading to human diseases. Overall an attempt has been made to highlight the importance of studying the genetic profiles of an individual with many factors.

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Platelet Activation in Stored Platelet Concentrates: Comparision of Two Methods Preparation

Cited by 2 publications

References 15 publications

Platelet‐Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro

Platelet‐Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro

The Influence of Environmental and Genetic Factors on Various Disorders and Diseases

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