Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations

Abstract

The aim of this study was to evaluate the trochlear bone and cartilaginous regeneration of rabbits using the association of PRP, chitosan, and hydroxyapatite. Hole was made in rabbit troches, one hole in each animal remained empty (group C), and one was filled by a combination of PRP, chitosan, and hydroxyapatite (group T). Clinical-orthopedic, radiographic, and histomorphometric evaluations were performed. Clinical-orthopedic evaluation showed lameness of two members of the T group and one member of group C. The radiographic evaluation showed that the T group showed absence of subchondral bone reaction (33%). The presence of moderate subchondral bone reaction was more frequently reported in group C with 67%. Microscopic evaluation revealed a presence of tissue neoformation, composed of connective tissue. Microscopic findings were similar in both groups, with a difference in the amount of neoformed tissue being perceptible, which was confirmed after the morphometric analysis, which revealed a significant difference in the quantity of newly formed tissue at the bone/cartilage/implant interface. The composite base of the association of chitosan, hydroxyapatite, and platelet-rich plasma favored bone and cartilage healing.

In vivo bone formation assessment using PRP with chitosan (Animal comparative study)

“…And the result agrees with Sequando et al, 2018 and Shwu et al, 2009 that used the mixture of chitosan and PRP with other CASO4 and showed more bone formation compared with chitosan alone [19,20].…”

Improvising PRP for use in osteoarthritis knee- upcoming trends and futuristic view

“…They hypothesized that Chitosan/PRP reside for several weeks in vivo, in contrast to PRP which is quickly degraded. Segundo et al 40 in their study on surgically created cartilage defects in rabbit knees noted that composite base of PRP, hydroxyapatite and chitosan promoted better bone healing and cartilage healing compared to control. There is encouraging in vitro and in vivo evidence that PRP can be used with biomaterials to enhance their effect, however whether this can be translated to actual clinical scenarios remains to be seen.…”

Synthesis and in vivo evaluation of a scaffold containing wollastonite/β‐TCP for bone repair in a rabbit tibial defect model

“…The production of bioceramic implants that allow its association seems to be the most efficient method of its use, its longer time of biodegradation and its performance in a more specific step of the bone repair compensated by the properties of other polymers, these associations have been showing good results (Segundo FA de et al, ).…”