Platelet-rich Plasma (PRP) in Orthopedics and Traumatology — Review

Kossev, Pencho; Sokolov, Tsvetan

doi:10.5772/61326

Cited by 6 publications

(4 citation statements)

References 85 publications

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“…До складу PRF входять 97 % тромбоцитів та 50 % (Kiran et al, 2011;Kossev & Sokolov, 2015;Yaltirik et al, 2018) лейкоцитів, які розміщені у тривимірному структурованому фібриновому каркасі та здатні впливати на проліферацію і міграцію інших клітин, що беруть участь у репаративних процесах .…”

Section: вступunclassified

“…Відомо (Kossev & Sokolov, 2015;Öncü et al, 2016), що кількість тромбоцитів у PRP здатна впливати на репаративні процеси таким чином: стимулюючи у кількості від 800-1500 ×10 9 /л та інгібуючи, коли цей показник перевищує 5000 ×10 9 /л. Проведене дослідження демонструє, що PRP, одержана при 100 g, хоч і мала доволі незначний об'єм, все ж містила необхідну кількість клітин для індукції репаративного потенціалу.…”

Section: обговоренняunclassified

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Histological characteristics of Platelet-Rich Fibrin clots obtained under various modes of blood centrifugation

Shevchenko

Rublenko

2020

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Autologous products of the first and second generation, namely platelet-rich plasma and platelet-rich fibrin, are considered promising for regenerative medicine. They differ from each other in physical properties, as well as in the way they are obtained. The key procedure of all techniques is centrifugation; changing its parameters affects the biological properties of these biomaterials. The aim of the work is to determine and histologically characterize the area of concentration cells of autologous fibrin enriched with platelets, depending on the change in centrifugation parameters. The studies were carried out on rabbits. Blood was collected and platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) were obtained using different values of relative centrifugal force: 100 g, 400 g, 735 g, 906 g, 1843 g. Due to the fact that it is impossible to determine the number of platelets in PRF clots, the counting was performed in platelet-rich plasma obtained by a single centrifugation with the corresponding parameters that were used to obtain PRF. The length of the formed clots was compared and a histological assessment of the cell composition in different layers (lower, middle and upper) was carried out. The highest platelet concentrations were observed in PRP obtained at 100 g and 400 g. Application of different values of centrifugal force showed obvious differences in the formation of platelet-rich fibrin clots. After preparation of I-PRF, its volume was significantly less than that of standard PRF, and the border between erythrocytes was less distinct. During the histological examination of fibrin clots, a change in the distribution of cellular elements in different parts was found with a change in the centrifugation parameters. With an increase in the parameter of relative centrifugal force, the length of the fibrin clot significantly increases, but the concentration of platelets in it significantly decreases. That is, it was found that the most optimal value of the relative centrifugal force for obtaining platelet mass is 100 g, which makes it possible to achieve the number of platelets greater than 800×109/L.

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Section: вступunclassified

Section: обговоренняunclassified

Histological characteristics of Platelet-Rich Fibrin clots obtained under various modes of blood centrifugation

Shevchenko

Rublenko

2020

SMLNUVMB

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“…Hematopoietic stem cells in the red bone marrow give rise to common myeloid progenitor cells which further differentiate to megakaryocytes . Platelets are anucleated products formed from long extensions into vascular sinusoids after migration of the megakaryocytes to the vascular niche . Vascular injury leads to exposure of prothrombotic extracellular matrix proteins, which facilitate platelet adhesion and activation.…”

Section: Overview Of Platelet Biologymentioning

confidence: 99%

Platelet biology in regenerative medicine of skeletal muscle

2018

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Platelet-based applications such as platelet-rich plasma (PRP) and platelet releasate have gained unprecedented attention in regenerative medicine across a variety of tissues as of late. The rationale behind utilizing PRP originates in the delivery of key cytokines and growth factors from α-granules to the targeted area, which in turn act as cell cycle regulators and promote the healing process across a variety of tissues. The aim of the present review is to assimilate current experimental evidence on the role of platelets as biomaterials in tissue regeneration, particularly in skeletal muscle, by integrating findings from human, animal and cell studies. This review is composed of 3 parts: firstly, we review key aspects of platelet biology that precede the preparation and use of platelet-related applications for tissue regeneration. Secondly, we critically discuss relevant evidence on platelet-mediated regeneration in skeletal muscle focusing on findings from (i) clinical trials, (ii) experimental animal studies and (iii) cell culture studies; and thirdly, we discuss the application of platelets in the regeneration of several other tissues including tendon, bone, liver, vessels and nerve. Finally, we review key technical variations in platelet preparation that may account for the large discrepancy in outcomes from different studies. This review provides an up-to-date reference tool for biomedical and clinical scientists involved in platelet-mediated tissue regenerative applications.

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“…PRP was introduced in the early 1950s, and is used in many areas of medicine today. 1 The use of PRP is beneficial and may be even more important in the future. The most common indications were tendon damage (77%), osteoarthritis (68%), muscle damage (57%) and cartilage damage (51%).…”

Section: Introductionmentioning

confidence: 99%

Platelet-rich plasma (PRP) on orthopedic clinical daily practice: a literature review

Gunawan¹,

Kesuma²,

Saputra¬¬³

et al. 2022

Intisari Sains Medis

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The use of PRP (platelet-rich plasma) is beneficial and even more important in the future. The most common indications were tendon damage (77%), osteoarthritis (68%), muscle damage (57%) and cartilage damage (51%). It is a purified autologous blood product with a higher platelet concentration than whole blood. It is rich in various cytokines and growth factors and has been shown to initiate and enhance healing by stimulating cell migration, cell proliferation, angiogenesis, and matrix synthesis. In rotator cuff injury, PRP injection led to significant long-term pain relief but not in functional improvements. In Osteoarthritis, PRP injection is associated with long-term pain relief and function improvements. PRP injection has a better outcome in VAS (visual analog scale) and DASH (The Disabilities of the Arm, Shoulder and Hand) scores in elbow ulnar collateral ligament pathologies, including lateral elbow epicondylitis. In medial epicondylitis, a paucity of studies causing therapeutic modality for PRP has not yet been established. In acute muscle injury, PRP used has no differences in imaging, pain, ROM (range of motion), muscle strength, and muscle function. In Achilles Tendinopathy, PRP injection has been associated with improvement in VAS, but no difference in VISA-A (The Victorian Institute of Sports Assesment – Achilles Questionnaire) score. However, the majority of published literature is secondary and tertiary evidence. Future studies could provide important information about the optimal concentrations of growth factors, platelets and leukocytes required for the desired effect in many specific tissues, as well as the appropriate method of administration and timing of drug application.

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Platelet-rich Plasma (PRP) in Orthopedics and Traumatology — Review

Cited by 6 publications

References 85 publications

Histological characteristics of Platelet-Rich Fibrin clots obtained under various modes of blood centrifugation

Histological characteristics of Platelet-Rich Fibrin clots obtained under various modes of blood centrifugation

Platelet biology in regenerative medicine of skeletal muscle

Platelet-rich plasma (PRP) on orthopedic clinical daily practice: a literature review

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