Platelet-Tumor Cell Hybrid Membrane-Camouflaged Nanoparticles for Enhancing Therapy Efficacy in Glioma

Abstract

Purpose Cell membrane-camouflaged nanoparticles (NPs) are drawing increasing attention because their surfaces acquire some characteristics of the cell membranes, making them a unique class of biomimetic materials for diverse applications. Modification of cell membrane or combination of different types of membranes can enhance their functionality. Methods We prepared platelet and tumor cell membrane camouflaged β-mangostin-loaded NPs, which were synthesized with platelet–C6 hybrid biomimetic coating, poly(lactic-co-glycolic acid), and β-mangostin (β-PCNPs). Then, we evaluated their targeting ability and anticancer activity against glioma in vitro and in vivo. Results Biomimetic coating enhanced active drug targeting and immune escape properties of nanocarrier in C6 and THP-1 cells, respectively, which improved their cytotoxicity. β-PCNPs were characterized to study the inherent properties of both source cells. Compared with bare β-NPs, β-PCNPs exhibited high tumor-targeting capability and induced apoptosis of C6 cells in vitro. Similarly, intravenous administration of drug through β-PCNPs resulted in enhanced tumor-targeting and exhibited excellent rate of inhibition of glioma tumor growth in mice. Moreover, the blood circulation time of drug in mice in the β-PCNP group was markedly prolonged and these mice exhibited better outcome than those in the β-NP group. Conclusion These results provide a new strategy of utilizing PCNPs as carriers for drug delivery, which improves the targeting efficiency and therapeutic efficacy of chemotherapeutic agents for glioma therapy.

Cell-Membrane-Coated Nanoparticles for Targeted Drug Delivery to the Brain for the Treatment of Neurological Diseases

“…In recent years, multifunctional hybrid cell membranes (HCMs) have begun to be used to fabricate HCMCNPs [ 108 , 109 ]. HCMs mostly employ two types of cell membranes to hybridize such that the HCMs possess unique biological functions.…”

“…HCMs mostly employ two types of cell membranes to hybridize such that the HCMs possess unique biological functions. The types of HCMs for current applications in NDs are shown in Figure 3 , and they include neutrophils–macrophages [ 110 ], dendritic cells–cancer cells [ 108 ], erythrocytes–cancer cells [ 111 , 112 ], and platelets–cancer cells [ 109 ]. HCMCNPs inherit the advantages of various cell membranes and make up for the deficiencies of different cell membranes.…”

Recent advances in biological membrane-based nanomaterials for cancer therapy

“…Recently, a large number of studies have reported the design of nanoparticles that mimic cell functions by using various biological membranes, thus mediating excellent antitumor therapy. Briefly, these membranes include (1) natural cell membranes, such as red blood cell membrane, [53][54][55][56] white blood cell membrane, 57,58 tumor cell membrane 55,59 and macrophage membrane, [60][61][62] (2) cell-secreted membrane structure, 63 such as exosome, 60,[64][65][66][67][68][69] bacterial outer membrane [70][71][72] and apoptotic body, 73 (3) membrane structure with particular protein expression or inclusions, [74][75][76] such as membrane expressed with tumor-targeting molecule, programmed death-ligand 1 (PD-L1) or programmed cell death protein 1 (PD-1), and (4) the hybrid membrane [77][78][79][80][81] formed by two or more kinds of the above membranes. Moreover, many studies have shown that if we combine anti-tumor drugs with biological membrane-based nanoparticles, we will get a better therapeutic effect than drug-free biological membrane-based nanomaterials.…”

Platelet-Vesicles-Encapsulated RSL-3 Enable Anti-Angiogenesis and Induce Ferroptosis to Inhibit Pancreatic Cancer Progress

“…In recent years, the function of biological cell membranes for drug delivery in vivo has been continuously expanded ( 30 ), and the cell membranes of red blood cells, platelets, macrophages and cancer cells have been used to manufacture drug delivery carriers ( 31 ). Extracellular vesicles (EVs) are derived from the cell membrane, can also regulate the transport of substances and information exchange between many cells ( 32 ).…”