Pleural lavage cytology immediately after thoracotomy and before closure of the thoracic cavity for lung cancer without pleural effusion and dissemination: Clinicopathologic and prognostic analysis

Higashiyama, Masahiko; Doi, Osamu; Kodama, Ken; Yokouchi, Hideoki; Tateishi, Ryuhei; Horai, Takeshi; Ashimura, Jun-ichi; Nagumo, Sachiko; Naruse, Yasuyoshi

doi:10.1007/bf02305554

Cited by 48 publications

(45 citation statements)

References 8 publications

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“…In Asian countries, the predominant histological type of esophageal carcinoma is squamous cell carcinoma, and tumors are frequently located in the thoracic esophagus. As in lung cancer (11)(12)(13)(14), the presence of exfoliated cancer cells in the pleural cavity after The results are presented as the means ± SEM. The isotonic NaCl buffer before cell suspension, n=5; the other solutions, n=3.…”

Section: Discussionmentioning

confidence: 99%

Pleural lavage with distilled water during surgery for esophageal squamous cell carcinoma

Kosuga

Shiozaki

Ichikawa³

et al. 2011

Oncol Rep

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Abstract. This study aimed to investigate cytocidal effects of hypotonic shock on esophageal squamous cell carcinoma (ESCC) cell lines, and to apply pleural lavage with distilled water to surgery for ESCC. Three human ESCC cell lines, TE5, TE9 and KYSE170 were exposed to distilled water, and morphological changes in ESCC cells were closely observed under a differential interference contrast microscope connected to a high-speed digital video camera. Further, serial cell volume changes after hypotonic shock were measured using a high-resolution flow cytometer. To investigate the cytocidal effects of hypotonic shock on ESCC cells, re-incubation of ESCC cells was performed after hypotonic shock. Additionally, the effects of 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB), a Cl -channel blocker, during hypotonic shock were analyzed. Video recordings by high-speed digital camera demonstrated that hypotonic shock with distilled water induced cell swelling followed by cell rupture. Measurements of cell volume changes using a high-resolution flow cytometer indicated that severe hypotonicity with distilled water increased broken fragments of ESCC cells within 5 min. Re-incubation experiments demonstrated cytocidal effects of hypotonic shock on ESCC cells. Treatment of cells with NPPB increased cell volumes by the inhibition of regulatory volume decrease, which is observed during hypotonic shock, and enhanced cytocidal effects. These findings demonstrated the cytocidal effects of hypotonic shock on ESCC cells, and clearly support the efficacy of pleural lavage with distilled water during surgery for ESCC.

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Section: Discussionmentioning

confidence: 99%

Pleural lavage with distilled water during surgery for esophageal squamous cell carcinoma

Kosuga

Shiozaki

Ichikawa³

et al. 2011

Oncol Rep

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“…Spread of malignancies to the pleural cavity, resulting in malignant pleural effusions and pleural metastatic disease are associated with a dismal prognosis, with most patients surviving only a few months after the diagnosis is made (40)(41)(42)(43)(44)(45)(46)(47). While NSCLC is the most common cause of malignant disease of the pleura, the pleural model used in this study is also relevant to other cancer types, including mesothelioma, breast, ovarian, and lymphoid cancers (48)(49)(50).…”

Section: Discussionmentioning

confidence: 99%

“…Such foci of disease are extremely difficult to diagnose preoperatively. Metastatic disease in the pleural cavity may indicate a more aggressive tumor biology an a higher risk of recurrence (12)(13)(14)(15)(16), which could contribute to the decreased survival often seen in these patients. As such, new techniques are needed to both diagnose and treat metastatic pleural spread of NSCLC.…”

Section: Introductionmentioning

confidence: 99%

Minimally invasive localization of oncolytic herpes simplex viral therapy of metastatic pleural cancer

et al. 2005

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Purpose-Herpes simplex virus-one (HSV-1) oncolytic therapy and gene therapy are promising treatment modalities against cancer. NV1066, one such HSV-1 virus carries a marker gene for enhanced green fluorescent protein (EGFP). The purpose of this study was to determine whether NV1066 is cytotoxic to lung cancer and whether EGFP is a detectable marker of viral infection in vitro and in vivo. We further investigated whether EGFP expression in infected cells can be used to localize the virus and to identify small metastatic tumor foci (< 1 mm.) in vivo by means of minimally invasive endoscopic systems equipped with fluorescent filters.Experimental Design-In A549 human lung cancer cells, in vitro viral replication was determined by plaque assay, cell kill by LDH release assay, and EGFP expression by flow cytometry. In vivo, A549 cells were injected into the pleural cavity of athymic mice. Mice were treated with intrapleural injection of NV1066 or saline and examined for EGFP expression in tumor deposits using a stereomicroscope or a fluorescent thoracoscopic system. Results-NV1066 replicated in, expressed EGFP in infected cells and killed tumor cells in vitro.In vivo, treatment with intrapleural NV1066 decreased pleural disease burden, as measured by chest wall nodule counts and organ weights. EGFP was easily visualized in tumor deposits, including microscopic foci, by fluorescent thoracoscopy.Conclusions-NV1066 has significant oncolytic activity against a human NSCLC cell line and is effective in limiting the progression of metastatic disease in an in vivo orthotopic model. By incorporating fluorescent filters into endoscopic systems, a minimally-invasive means for diagnosing small metastatic pleural deposits and localization of viral therapy for thoracic malignancies may be developed using the EGFP marker gene inserted in oncolytic herpes simplex viruses.

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“…9 Briefly, when no malignant findings were noted, the pleural cavity was washed with 200 ml of physiological saline solution, most then collected into a glass bottle with a mixture of heparin. After centrifugation, the sediment was stained using Giemsa, Papanicolaou, and Alcian blue on 8 glass slides.…”

Section: Methodsmentioning

confidence: 99%

“…Several investigators, including ourselves, [5][6][7][8][9][10][11][12][13] have reported intraoperative pleural lavage cytology (PLC) -positive lung cancer patients, despite a lack of macroscopic findings of malignant pleuritis at thoracotomy. Overall, 3.7 to 45.7% of operable lung cancer pa- Table I.…”

mentioning

confidence: 99%

Clinical value of pleural lavage cytological positivity in lung cancer patients without intraoperative malignant pleuritis

Higashiyama

Kodama

Yokouchi

et al. 2000

Jpn J Thorac Caridovasc Surg

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Pleural lavage cytology immediately after thoracotomy and before closure of the thoracic cavity for lung cancer without pleural effusion and dissemination: Clinicopathologic and prognostic analysis

Abstract: PLC status after thoractomy provides useful information in the detection of high-risk subgroup for pleural recurrence. Although PLC status is closely associated with survival, its prognostic value is not independent.

Cited by 48 publications

References 8 publications

Pleural lavage with distilled water during surgery for esophageal squamous cell carcinoma

Pleural lavage with distilled water during surgery for esophageal squamous cell carcinoma

Minimally invasive localization of oncolytic herpes simplex viral therapy of metastatic pleural cancer

Clinical value of pleural lavage cytological positivity in lung cancer patients without intraoperative malignant pleuritis

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