PLGA-Based Nano-Encapsulation of <i>Trachyspermum Ammi</i> Seed Essential Oil (TSEO-PNP) as a Safe, Natural, Efficient, Anticancer Compound in Human HT-29 Colon Cancer Cell Line

Almnhawy, Mokhalad; Jebur, Mohammed Sh.; Alhajamee, Maitham; Marai, Khadeeja; Tabrizi, Masoud Homayouni

doi:10.1080/01635581.2020.1862256

Cited by 16 publications

(8 citation statements)

References 44 publications

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“…This is due to various inventions regarding the therapeutic use of individual plants. The plant T. ammi serves not only as antioxidant, antispasmodic effects, its anti-cancer effect also evidenced in previous articles [55]. Thymol, one of the constituents of ajwain, induces apoptosis in MCF-7 and affects the gene expression in p53 gene in breast cancer cell line [56].…”

Section: Discussionmentioning

confidence: 64%

Trachyspermum ammi Seed Ethanolic Extract Inhibits Cell Proliferation on A549 Lung Cancer Cell: An In-vitro Analysis

Subramanian

Ezhilarasan

et al. 2021

JPRI

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Introduction: Lung cancer is the second most common type of cancer, accounting for one in every five male cancers and one in every nine female cancers. Lung cancer treatment is dictated by the cell type of the illness, the amount to which it has spread, and the patient's general condition. It is well understood that tumours confer resistance to chemotherapeutic drugs or radiation in part due to apoptotic pathway malfunction in cancer cells. Aim: The aim of this study was to investigate the in vitro anti proliferative activity of the ethanolic extract of Trachyspermum ammi (T.ammi) seeds against A549 adenocarcinomic human alveolar basal epithelial cells. Materials and Methods: The cytotoxicity and anti-cancer effects of ethanolic extract of Trachyspermum ammi (T.ammi) seeds against A549 cells were analyzed using MTT assay and morphological analysis by inverted phase contrast microscopy. Results: The MTT assay results showed that 50% of the cell proliferation (IC50) has been inhibited upon T.ammi seed extract treatment for 24hrs incubation. The Dose-dependent studies revealed cytotoxic dose level IC50 of 50μg/ml for T.ammi seed extract on lung cancer cells. It clearly showed that the dose dependent treatments significantly (p<0.001) reduces the cell viability thereby inhibiting the cancer cell proliferation when compared and it was further confirmed with morphological evaluation with microscopic study. Conclusion: From this study we concluded that the ethanol extract of T.ammi significantly inhibits cancer cell proliferation against A549 cells and it might be a potent anti-proliferative value for further evaluation to determine the therapeutic agent for cancer treatment.

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Section: Discussionmentioning

confidence: 64%

Trachyspermum ammi Seed Ethanolic Extract Inhibits Cell Proliferation on A549 Lung Cancer Cell: An In-vitro Analysis

Subramanian

Ezhilarasan

et al. 2021

JPRI

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“…Finally, the absorbance of the samples was read at a wavelength of 539 nm. It is worth noting that FeSo 4 was used as the standard and drawing the standard curve 15 …”

Section: Methodsmentioning

confidence: 99%

Evaluation of the effect of designed PLGA‐arctiin nanoparticles modified with folic acid and chitosan on colon cancer cells

Alhadidi,

Tabrizi,

Ghobeh

2023

Biotech and App Biochem

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In this study, we designed nanoparticles (NPs) based on polylactic acid glycolic acid modified with chitosan and folic acid to optimize the anti‐cancer, anti‐inflammatory, and antioxidant effects of arctiin (ARC), and we measured its effects on cancer cells, including colon cancer. NPs were synthesized using the W1/O/W2 double‐emulsion solvent evaporation method. Physicochemical characteristics of synthesized NPs (ARC‐PCF‐NPs), including average particle size, dispersity index (PDI), zeta potential (ZP), field emission scanning electron microscope figures, and encapsulation efficiency (EE), were evaluated. 2,2′‐Azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS), 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) methods were carried out to determine the antioxidant properties of NPs. 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐2H‐tetrazolium bromide assay was performed to investigate cytotoxicity effects on cancer cells and normal fibroblasts. Quantitative polymerase chain reaction was also performed on inflammatory and antioxidant genes. The obtained results indicated that the synthesized NPs have a size of 100 nm, a DPI of 0.36, a ZP of 26.30 mV, and EE was calculated at about 87.5%. The antioxidant influence of ARC‐PCF‐NPs was confirmed by inhibiting ABTS and DPPH free radicals and ferrous reduction in the FRAP method. Moreover, the reduction of inflammatory and antioxidant genes confirmed the anti‐inflammatory and antioxidant properties of NPs. These results indicate the modification of the surface of NPs in order to increase the bioavailability, stability, and effectiveness of medicinal compounds in therapeutic applications.

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“…TPNs exhibited high targetability as they specifically suppressed the growth of HT-29 cancer cells and exhibited anti-angiogenesis effects. Their cytotoxicity was found to be dose-and time-dependent against the HT-29 cells [47]. Chittasupho et al developed LFC131(peptide)-linked sodium carboxymethyl cellulose-layered PLGA NPs (LFC131-DOX-SPNs) for targeted delivery of DOX against CXCR4-overexpressed lung cancerous cells.…”

Section: Plga-based Nanomaterialsmentioning

confidence: 99%

Polyester nanomedicines targeting inflammatory signaling pathways for cancer therapy

Das

Singh

Verma

et al. 2022

Biomedicine & Pharmacotherapy

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PLGA-Based Nano-Encapsulation of Trachyspermum Ammi Seed Essential Oil (TSEO-PNP) as a Safe, Natural, Efficient, Anticancer Compound in Human HT-29 Colon Cancer Cell Line

Cited by 16 publications

References 44 publications

Trachyspermum ammi Seed Ethanolic Extract Inhibits Cell Proliferation on A549 Lung Cancer Cell: An In-vitro Analysis

Trachyspermum ammi Seed Ethanolic Extract Inhibits Cell Proliferation on A549 Lung Cancer Cell: An In-vitro Analysis

Evaluation of the effect of designed PLGA‐arctiin nanoparticles modified with folic acid and chitosan on colon cancer cells

Polyester nanomedicines targeting inflammatory signaling pathways for cancer therapy

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