PM 7/40 (3) <i>Globodera rostochiensis</i> and <i>Globodera pallida</i>

doi:10.1111/epp.12025

Cited by 24 publications

(6 citation statements)

References 25 publications

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“…The results in this study clearly demonstrate that the Globodera populations present in Portuguese potato fields are morphologically variable, with some features outside the ranges expected for G. pallida or G. rostochiensis, making it difficult to identify PCN species when cysts and J2 are individually analysed. However, morphometric average values (Table 2) were within the range provided in the literature (Marks & Brodie, 1998;EPPO, 2013). According to the obtained morphometric data, morphological characterisation did not always match the molecular identification (as shown by comparing the morphological characteristics typical of G. rostochiensis species of samples nos 7, 11, 13, 18, 24, 27 and 36 in Table 1 and their molecular identification as G. pallida in Table 3.…”

Section: Discussionsupporting

confidence: 56%

“…The morphological characters of the 40 cysts and 40 J2 from Portuguese populations did not always match the descriptions of G. rostochiensis and G. pallida (Table 1), but the morphometric average values were within the range described in the literature (Marks & Brodie, 1998;EPPO, 2013) (Table 2).…”

Section: Morphology and Morphometricsmentioning

confidence: 70%

“…Pictures were taken with an Olympus DP10 digital camera. The observed characters of cysts and J2 were compared with those from the reference materials and descriptions in the literature (Marks & Brodie, 1998;EPPO, 2013). Measurements were made using a ProgResSpeed XT core 5 -Jenoptik image software and dimensions are expressed in μm.…”

Section: Morphological Characterisationmentioning

confidence: 99%

“…The amplification profile for ITS-rRNA consisted of an initial denaturation of 94°C for 2 min followed by 35 cycles of 94°C for 30 s, 55°C for 30 s and 72°C for 30 s and a final extension of 72°C for 7 min (EPPO, 2013). The amplified products were loaded onto a 1.5% agarose gel containing 0.5 μg · ml −1 ethidium bromide and 0.5×Trisborate-EDTA (TBE) running buffer and electrophoresed at 5 V/cm.…”

Section: Multiplex Pcr By Species-specific Primersmentioning

confidence: 99%

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Morphological and molecular identification of the potato cyst nematodes Globodera rostochiensis and G. pallida in Portuguese potato fields

Camacho

Nóbrega

Lima

et al. 2017

Nematol

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The potato cyst nematodes (PCN) Globodera rostochiensis and G. pallida pose one of the greatest threats to potato crops worldwide and are subject to strict quarantine regulations in many countries. The identification of these Globodera species based on morphology may be ambiguous due to the variability of the main morphological features and the overlapping of the standard parameters in these two species; thus, confirmation via molecular methods is recommended. Multiplex PCR with speciesspecific primers (ITS5/PITSp4 + PITSr3) allows both species to be distinguished. However, despite the development of molecular identification methods, the morphological approach remains useful as a complementary diagnostic technique. In this work, we report results of morphological and molecular analyses that were carried out in two Globodera species from Portuguese potato fields. The average morphometric values of 40 cysts and 40 second-stage juveniles were generally within the expected ranges for G. pallida and G. rostochiensis with some variations noted. Molecular analysis with multiplex PCR confirmed the morphometric identification. The present results confirmed the occurrence of two potato cyst nematode species, G. rostochiensis and G. pallida. Surprisingly, the analysis of soils from Portuguese potato fields detected a greater number of samples infested with G. pallida, which is contrary to expectation as G. rostochiensis has been considered the most widespread species in Portugal. The distinction between the two species is therefore essential in order to detect their presence in the country with a view to re-evaluating the control measures implemented so far and adopting more effective practices.

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Section: Discussionsupporting

confidence: 56%

Section: Morphology and Morphometricsmentioning

confidence: 70%

Section: Morphological Characterisationmentioning

confidence: 99%

Section: Multiplex Pcr By Species-specific Primersmentioning

confidence: 99%

See 2 more Smart Citations

Morphological and molecular identification of the potato cyst nematodes Globodera rostochiensis and G. pallida in Portuguese potato fields

Camacho

Nóbrega

Lima

et al. 2017

Nematol

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“…Globodera pallida originated in South America and is now widely distributed in 55 countries. Yield losses of potato due to G. pallida range from 50 to 80% in heavily infested soils [54]. Although the beet cyst nematode, Heterodera schachtii, is a primary pathogen of sugar beets, it can parasitize plant species in 23 diferent plant families with losses of 30% in the families of Chenopodiaceae [55,56].…”

Section: Cyst Nematodesmentioning

confidence: 99%

The Impact of Plant-Parasitic Nematodes on Agriculture and Methods of Control

Bernard¹,

Egnin²,

Bonsi³

2017

Nematology - Concepts, Diagnosis and Control

128

101

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Plant-parasitic nematodes are costly burdens of crop production. Ubiquitous in nature, phytoparasitic nematodes are associated with nearly every important agricultural crop and represent a signiicant constraint on global food security. Root-knot nematodes (Meloidogyne spp.) cyst nematodes (Heterodera and Globodera spp.) and lesion nematodes (Pratylenchus spp.) rank at the top of list of the most economically and scientiically important species due to their intricate relationship with the host plants, wide host range, and the level of damage ensued by infection. Limitations on the use of chemical pesticides have brought increasing interest in studies on alternative methods of nematode control. Among these strategies of nonchemical nematode management is the identiication and implementation of host resistance. In addition, nematode genes involved in parasitism represent key targets for the development of control through gene silencing methods such as RNA interference. Recently, transcriptome proiling analyses has been used to distinguish nematode resistant and susceptible genotypes and identify the speciic molecular components and pathways triggered during the plant immune response to nematode invasion. This summary highlights the importance of plant-parasitic nematodes in agriculture and the molecular events involved in plant-nematode interactions.

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Agricultural waste amendments improve inundation treatment of soil contaminated with potato cyst nematodes, Globodera rostochiensis and G. pallida

et al. 2016

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Potato cyst nematodes (PCN), Globodera rostochiensis and G. pallida, are major constraints to potato production. They are distributed passively with soil adhering to farm machinery and harvested tubers, but also by waste soil from trading and processing plants. Inundation is considered an environmentally save method for disinfestation of waste soil. We investigated if agro-industrial waste products accelerate this disinfestation process by studying PCN survival, hatching and infectivity over time. Experiments were conducted in 2-L containers filled with 1.5 L non-amended soil, soil amended with steamed or fresh potato peels, calcium sulphate, steamed potato peels mixed with calcium sulphate, or leek leaves. In each container, cysts of G. rostochiensis or G. pallida were buried in the soil in retrievable bags and 400 ml water was added. Non-amended and non-inundated soils were the controls. It took 8 weeks of inundation of non-amended soil to reduce nematode survival by 72 %, while in amended soils survival was reduced up to 99.9 % after 4 weeks. After 1 week, fewer second-stage juveniles (J2) hatched and infected roots in inundated amended soil than in inundated non-amended soil. Host finding ability of surviving J2 was not affected. To understand mechanisms of nematode suppression, concentrations of short chain fatty acids (SCFA), gases (O-2, CO2, H2S, CH4, and N-2) and phospholipid fatty acids (PLFAs) were determined. Higher concentrations of SCFA and carbon dioxide and lower levels of oxygen were detected in amended treatments. H2S was occasionally detected in the leek-amended soil. The microbial biomass changed significantly in potato peels-amended soils

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PM 7/40 (3) Globodera rostochiensis and Globodera pallida

Abstract: Specific scope This standard describes a diagnostic protocol for Globodera rostochiensis and Globodera pallida. Specific approval and amendment Approved as an EPPO Standard in 2003–09. Revision approved in 2009–09, 2nd revision approved in 2012‐09.

Cited by 24 publications

References 25 publications

Morphological and molecular identification of the potato cyst nematodes Globodera rostochiensis and G. pallida in Portuguese potato fields

Morphological and molecular identification of the potato cyst nematodes Globodera rostochiensis and G. pallida in Portuguese potato fields

The Impact of Plant-Parasitic Nematodes on Agriculture and Methods of Control

Agricultural waste amendments improve inundation treatment of soil contaminated with potato cyst nematodes, Globodera rostochiensis and G. pallida

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