Pneumonia in pigs infected with pseudorabies virus and haemophilus parasuis serovar 4

Narita, Minoru; Kawashima, Kenji; Matsuura, Shouhei; Uchimura, A.; Miura, Yasuo

doi:10.1016/s0021-9975(08)80310-6

Cited by 26 publications

(15 citation statements)

References 11 publications

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“…1 Bacterial antigen was detected within the cytoplasm of neutrophils, interstitial macrophages, and alveolar macrophages in the first study 7 and within inflammatory cells and the lumina and endothelial cells of small blood vessels in several tissues in the second study. 1 In the present study, positive staining appeared within macrophage-like cells and neutrophils and was disseminated through fibrin fibers.…”

Section: Discussionmentioning

confidence: 94%

“…A similar technique was used in 2 previous studies involving the detection of H. parasuis antigen in bronchopneumonic cases in association with pseudorabies virus infection 7 and in several tissues of pigs experimentally infected with H. parasuis serovars 1, 4, and 5.…”

Section: Discussionmentioning

confidence: 99%

“…In other studies using antisera against H. parasuis, cross-reactivity with A. pleuropneumoniae was not investigated. 7,19 Cross-reactivity could be due to the close relationship of these bacteria. 11,16 In a numerical taxonomic analysis (based on the biochemical reactivity on API20E and API ZYM test strips) using dendrograms that compared the similarity of H. parasuis, A. pleuropneumoniae, Pasteurella multocida, and Bordetella bronchiseptica, a similarity of 86% between H. parasuis and A. pleuropneumoniae was found, but in some cases comparing specific strains of these bacteria similarity reached 91% and 93%.…”

Section: Discussionmentioning

confidence: 99%

“…Some workers have used an immunohistochemistry technique for detecting H. parasuis antigen in tissues of infected animals; 1,7 however, no clear conclusions have been presented as regards the correlation of these results with those of bacterial isolation. The immunohistochemical technique seemed to be sensitive and specific.…”

mentioning

confidence: 99%

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Immunohistochemical Detection ofHaemophilus ParasuisSerovar 5 in Formalin-Fixed, Paraffin-Embedded Tissues of Experimentally Infected Swine

et al. 1997

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Abstract. An avidin-biotin complex immunohistochemistry technique was developed to detect Haemophilus parasuis serovar 5 in experimentally infected 18-21-day-old conventional pigs, using a rabbit polyclonal antiserum. Seven of 10 intratracheally inoculated animals developed a low to medium degree of fibrinous polyserositis; meninges and pleura were the most severely affected areas. Haemophilus parasuis was recovered from 9 of 10 pigs; in 2 of them H. parasuis was isolated from tracheal swabs only. Positive immunohistochemistry results, mainly observed as free bacteria or bacteria within inflammatory cell cytoplasm in the fibrinopurulent exudate, were observed in 8 of 10 animals. Cross-reactivity with Actinobacillus pleuropneumoniae was detected but not with other gram-positive and gram-negative bacteria tested. This immunohistochemistry technique seemed to be at least as sensitive as microbiologic cultures and could be useful in studies of pathogenesis and retrospective diagnosis. However, cross-reactivity with A. pleuropneumoniae means that positive immunohistochemistry results in lung tissue from field cases would be dubious.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Immunohistochemical Detection ofHaemophilus ParasuisSerovar 5 in Formalin-Fixed, Paraffin-Embedded Tissues of Experimentally Infected Swine

et al. 1997

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“…PRV infection in AM may induce immunosuppression and interfere with bacterial clearance in the lung. The pigs infected with PRV plus Pasteurella multocida [8], Streptococcus suis [13] or Haemophilus parasuis [20] caused severe pneumonia. Recently, Sakano et al [22] reported that the pigs inoculated with PRV plus Actinobacillus pleuropneumoniae type 1 developed severe pneumonia than A. pleuropneumoniae alone and with PRV alone developed no pneumonia.…”

Section: Methodsmentioning

confidence: 99%

Experimental Dual Infection of Cesarean-Derived, Colostrum-Deprived Pigs with Mycoplasma hyopneumoniae and Pseudorabies Virus.

et al. 1998

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ABSTRACT. To determine whether pseudorabies virus (PRV) infection increases the severity of pneumonia by Mycoplasma hyopneumoniae, 18, 10-week-old Cesarean-derived, colostrum-deprived pigs were randomly assigned to 3 groups of 6 pigs each. Pigs in groups A and C were inoculated intranasally with M. hyopneumoniae at 10-week-old. At 11-week-old, pigs in groups B and C were inoculated intranasally with PRV. All pigs were initially seronegative for M. hyopneumoniae and PRV. Three pigs of each group were euthanized at 12-weekold, and remaining pigs at 14-week-old. At necropsy, gross lesions in the lung were observed in the pigs of groups A and C. On postinoculation-week (PIW) 2 with M. hyopneumoniae (at 12-week-old), lung lesions were recognized in one of the 3 pigs in group A and all the pigs in group C. The mean percentage of the lung lesions were 0.1% in group A and 9.8% in group C. M. hyopneumoniae was isolated from broncho-alveolar lavage fluids (BALF) of pigs in group A with titer of 10 2 to 10 3 CCU/0.2 ml and in group C with titer of 10 5 to 10 6 CCU/0.2 ml. On PIW 4 (at 14-week-old), lung lesions were observed in all the pigs in groups A and C, and the mean percentage of the lung lesions were 8.3% in group A and 17.2% in group C. M. hyopneumoniae was isolated from BALF in group A with titer of 10 4 to 10 7 CCU/0.2 ml and in group C with titer of 10 6 to 10 7 CCU/0.2 ml. PRVs were isolated from nasal swab and tissue samples in groups B and C. After inoculation, antibody against M. hyopneumoniae was detected in groups A and C, and against PRV in groups B and C. Under the present experimental conditions, PRV infection appear to have effect on the severity of experimentally induced acute mycoplasmal pneumonia in young pigs. -KEY WORDS: BALF, dual infection, Mycoplasma hyopneumoniae, pseudorabies virus, swine.

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Bacterial Infections are Potentiated by Porcine Reproductive and Respiratory Syndrome Virus Infection: Fact or Fiction?

Segalés

McCaw

Morilla

et al. 2002

Trends in Emerging Viral Infections of Swine

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Pneumonia in pigs infected with pseudorabies virus and haemophilus parasuis serovar 4

Cited by 26 publications

References 11 publications

Immunohistochemical Detection ofHaemophilus ParasuisSerovar 5 in Formalin-Fixed, Paraffin-Embedded Tissues of Experimentally Infected Swine

Immunohistochemical Detection ofHaemophilus ParasuisSerovar 5 in Formalin-Fixed, Paraffin-Embedded Tissues of Experimentally Infected Swine

Experimental Dual Infection of Cesarean-Derived, Colostrum-Deprived Pigs with Mycoplasma hyopneumoniae and Pseudorabies Virus.

Bacterial Infections are Potentiated by Porcine Reproductive and Respiratory Syndrome Virus Infection: Fact or Fiction?

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