PO-330 Prevention of worsening in glioma relapse

Abstract

selective condition, the effects of the mutation of interest can be analysed by measuring the proportion of each barcode in genomic DNA by real-time quantitative PCR or deep sequencing. Results and discussions We used this CRISPR-barcoding strategy to model different mechanisms of non-small-cell lung cancer resistance to EGFR inhibitors, and we established a multiplex system to evaluate the efficacy of combined drug therapies aimed at preventing or delaying the emergence of resistant cells. Through a similar approach, we assessed the effects of repairing oncogenic driver mutations in addicted cancer cells directly at the genome level. Finally, we used a highly complex set of CRISPR-barcodes to trace intratumor heterogeneity, as a convenient alternative strategy to lentiviral barcode libraries. Conclusion CRISPR-barcoding is a fast and highly flexible means to investigate the effects of different kinds of genomic modifications in a broad range of functional assays.