2018
DOI: 10.1152/ajprenal.00575.2017
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Podocyte-specific knockin of PTEN protects kidney from hyperglycemia

Abstract: Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) has proven to be downregulated in podocytes challenged with high glucose (HG), and knockout of PTEN in podocytes aggravated the progression of diabetic kidney disease (DKD). However, whether podocyte-specific knockin of PTEN protects the kidney against hyperglycemia in vivo remains unknown. The inducible podocyte-specific PTEN knockin (PPKI) mice were generated by crossing newly created transgenic loxP-stop- loxP-PTEN mice with podocin-iCreER mice.… Show more

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Cited by 25 publications
(18 citation statements)
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“…Loss of PTEN induces podocyte cytoskeletal rearrangement and also leads to the development of proteinuria and DKD [19]. Podocyte-specific knock in of PTEN protected against kidney injury in a diabetic mouse model [20]. PDK1 can promote cell proliferation through regulation of the Akt pathway [21,22].…”
Section: Discussionmentioning
confidence: 99%
“…Loss of PTEN induces podocyte cytoskeletal rearrangement and also leads to the development of proteinuria and DKD [19]. Podocyte-specific knock in of PTEN protected against kidney injury in a diabetic mouse model [20]. PDK1 can promote cell proliferation through regulation of the Akt pathway [21,22].…”
Section: Discussionmentioning
confidence: 99%
“…Inhibition of PTEN in DN featuring EMT resulted in F-actin fiber reorganization and proteinuria (95). Another study by Wang et al (89) showed that mice with podocyte-specific PTEN knock-in were protected from hyperglycemia-induced mesangial expansion and FP effacement. However, the study of functional consequence of MAGI-2 and ␤-catenin in podocyte EMT is still limited.…”
Section: Does Magi-2 Affect the Epithelial-to-mesenchymal Transition mentioning
confidence: 99%
“…The mice were raised under the conditions as described. 22 The mice were randomized to three groups: BKS + DMSO (n = 6), db/db + DMSO (n = 8), and db/db + Tubacin (n = 8). Control or diabetic mice were randomized to receive 5.0 mg/kg tubacin (Sigma -Aldrich) in 50 µL of DMSO or isometric DMSO alone by the tail vein injection every 2 days for 8 weeks.…”
Section: Animal Studiesmentioning
confidence: 99%
“…Conditionally immortalized mouse podocyte cell line (given by Dr Peter Mundel, University of Miami, FL, USA) was cultured and maintained as reported. 22 All experiments of podocytes were carried out after differentiation and maturation. Matured podocytes were identified by immunofluorescent staining of synaptopodin (1:100 diluted with 5% F I G U R E 1 Expression and activity of HDAC6 in podocytes of type 2 diabetic patients, db/db mice and AGE-treated podocytes.…”
Section: Cell Culture and Treatmentsmentioning
confidence: 99%
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