Poly(2-hydroxyethyl methacrylate)-based slabs as a mouse embryonic stem cell support

Horák, Daniel; Kroupová, Jana; Šlouf, Miroslav; Dvořák, Petr

doi:10.1016/j.biomaterials.2003.12.031

Cited by 51 publications

(43 citation statements)

References 25 publications

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“…Historically, salt particles are most commonly used because they are inexpensive, widely available, easy to handle, and stable under an assortment of processing conditions. Alternative porogen materials, including sugars, 25 paraffin, 26 and gelatin, 27 have also been employed with hydrogels. The nature of the porogen has been demonstrated to play an important role in the interconnectivity of pores and in turn the cellscaffold interactions after seeding.…”

Section: Solvent Casting=particle Leachingmentioning

confidence: 99%

Controlling the Porosity and Microarchitecture of Hydrogels for Tissue Engineering

Annabi

Nichol

Zhong

et al. 2010

Tissue Engineering Part B: Reviews

1,037

778

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Section: Solvent Casting=particle Leachingmentioning

confidence: 99%

Controlling the Porosity and Microarchitecture of Hydrogels for Tissue Engineering

Annabi

Nichol

Zhong

et al. 2010

Tissue Engineering Part B: Reviews

1,037

778

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“…Hydroxyethyl methacrylate based hydrogels are highly hydrophilic and biocompatible. These characteristics have enabled their use in biomedical field for applications such as contact lenses, drug delivery [10][11][12], and tissue engineering [13][14][15][16]. Owing to their high surface area, HEMA based hydrogels have found application also in environmental remediation such as removal of dyes from industrial waste waters [17,18], and in separation [19].…”

Section: Introductionmentioning

confidence: 99%

Hydroxyethyl Methacrylate Based Nanocomposite Hydrogels With Tunable Pore Architecture

Bat¹

2016

J. Turk. Chem. Soc., Sect. A: Chem.

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Hydroxyethyl methacrylate (HEMA)-based hydrogels have found increasing numbers of applications in areas such as chromatographic separations, controlled drug release, biosensing, and membrane separations. In all these applications, the pore size and pore interconnectivity are crucial for successful application of these materials as they determine the rate of diffusion through the matrix. 2-Hydroxyethyl methacrylate is a water-soluble monomer but its polymer, polyHEMA, is not. Therefore, during polymerization of HEMA in aqueous media, a porous structure is obtained as a result of phase separation. Pore size and interconnectivity in these hydrogels is a function of several variables such as monomer concentration, cross-linker concentration, temperature, etc. In this study, we investigated the effect of monomer concentration, graphene oxide addition or clay addition on hydrogel pore size, pore interconnectivity, water uptake, and thermal properties. PolyHEMA hydrogels were prepared by redox initiated free radical polymerization of the monomer using ethylene glycol dimethacrylate as a cross-linker. As a nanofiller, a synthetic hectorite Laponite® XLG and graphene oxide were used. Graphene oxide was prepared by the Tour Method. Pore morphology of the pristine HEMA based hydrogels and nanocomposite hydrogels were studied by scanning electron microscopy. The formed hydrogels were found to be highly elastic and flexible. A dramatic change in the pore structure and size was observed in the range between 22 to 24 wt/vol monomer at 0.5 % of cross-linker. In this range, the hydrogel morphology changes from typical cauliflower architecture to continuous hydrogel with dispersed water droplets forming the pores where the pores are submicron in size and show an interconnected structure. Such controlled pore structure is highly important when these hydrogels are used for solute diffusion or when there's flow through monolithic hydrogels. These robust hydrogels may be useful in separation and biomedical applications.

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“…Hence, hydrophilic surfaces resulted in smaller aggregates and hydrophobic surfaces in intermediate, regularly sized aggregates, which were found to exhibit sustained expression of germ-layer markers. Studies of individual polymers or other chemically defined biomaterials are informative in their specific context (Harrison et al, 2004a;Horák et al, 2004;Kroupová et al, 2006;Li et al, 2006). However, the pioneering polymer-array study (Anderson et al, 2004) showed that differences among polymers with respect to their effect on proliferation of hES cells in some cases depend upon soluble media supplements.…”

Section: Modified Organic Polymersmentioning

confidence: 99%

Topographically and Chemically Modified Surfaces for Expansion or Differentiation of Embryonic Stem Cells

Markert¹,

Lovmand²,

Duch³

et al. 2011

Methodological Advances in the Culture, Manipulation and Utilization of Embryonic Stem Cells for Basic and Practical Applicatio

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Poly(2-hydroxyethyl methacrylate)-based slabs as a mouse embryonic stem cell support

Cited by 51 publications

References 25 publications

Controlling the Porosity and Microarchitecture of Hydrogels for Tissue Engineering

Controlling the Porosity and Microarchitecture of Hydrogels for Tissue Engineering

Hydroxyethyl Methacrylate Based Nanocomposite Hydrogels With Tunable Pore Architecture

Topographically and Chemically Modified Surfaces for Expansion or Differentiation of Embryonic Stem Cells

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