Poly(diallyldimethylammonium chloride) as a Cationic Coating for Capillary Electrophoresis

Liu, Qicai; Lin, Fangming; Hartwick, Richard A.

doi:10.1093/chromsci/35.3.126

Cited by 59 publications

(41 citation statements)

References 19 publications

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“…Several coating procedures for the fused-silica capillary were evaluated to establish a fast and convenient protocol by which the coated surface, EOF, and migration time of analyte anions could be reproduced [4][5][6][19][20][21][22]. The coating procedure described in the Section 2.3 was found to produce the best results.…”

Section: Coating Proceduresmentioning

confidence: 99%

“…Previously, a capillary modified with poly(diallyldimethylammonium) chloride (PDDAC) has been applied successfully to the separation of both anions and proteins [4][5][6]. However, PDDAC also gave unsatisfactory results in terms of reproducibility under basic conditions where the capillary surface is altered by the increased dissociation of silanol groups.…”

Section: Introductionmentioning

confidence: 99%

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A new and selective capillary column coated with cationic diazacrown ether for separation of organic and inorganic anions by capillary electrophoresis

et al. 2003

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A new coated capillary has been introduced for capillary electrophoretic separation of anions by using a positively charged diazacrown ether with a 12-membered ring. A positive charge spread over the inner capillary surface led to a substantial anodic electroosmotic flow (EOF) over the range of migrating buffer of pH 2-11. Under the optimum conditions of 25 mM phosphate buffer at pH 7, the diazacrown-coated capillary showed a successful simultaneous separation of 7 inorganic anions and 13 aromatic anions (including positional isomers) in less than 15 min. The migration times of the sample anions and EOF marker for consecutive runs on a single column were highly reproducible, giving a relative standard deviation of 1%. Theoretical treatment of the migration behavior clearly demonstrated that ion association between the diazacrown and analyte anions is strongly dependent on the nature of the functional groups of anions (e.g., sulfonate groups > carboxyl groups) and the number of negative charges (e.g., trivalent anions > divalent anions > monovalent anions) on the analyte.

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Section: Coating Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A new and selective capillary column coated with cationic diazacrown ether for separation of organic and inorganic anions by capillary electrophoresis

et al. 2003

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“…In this last case, the capillary-coating procedure is reduced to a simple, three-step rinsing protocol, comprising deprotonation of surface silanols via bathing in a basic solution that will maximize the negative surface charge, adsorption of the polymer and a final rinse for removing excess polymer not adsorbed onto the surface. The polymeric coating comprise polyethylenimine [16], polybrene [17], poly(diallyldimethyl ammonium chloride) [18], polyarginine [19], chitosan [20], pyrrolidone-containing copolymers [21] quaternary ammonium-substituted agarose [22] or cationic coatings consisting of successive multiple ionic polymer layers [23]. In more recent times, a new cationic polyamine, made by copolymerizing 1,2-bis (3-aminopropylamino)ethane with epichlorohydrine, has been reported [24][25][26].…”

Section: Introductionmentioning

confidence: 99%

An N‐methylpolyvinylpyridinium cationic polymer for capillary coating in electrophoresis of proteins and peptides

et al. 2009

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A novel cationic polymeric coating is here reported, consisting of a N-methylpolyvinylpyridinium quaternary ion. This coating, combined with an isoelectric, aspartic acid as BGE, offers excellent separations of low- to high-M(r) proteins, of low- to high-pI values, coupled to very high run-to-run repeatability. The importance of a hydrophilic coating is also illustrated, whereas the N-methylpolyvinylpyridinium quaternary ion does not seem to adsorb even a large size protein as human albumin, separations are already distorted in the N-ethyl derivative and totally ruined in the N-octyl derivative, due to its high hydrophobicity.

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“…Strongly adsorbed semipermanent coatings can fill this requirement. Reversed EOF using the cationic polymer, poly-(diallyldimethylammonium chloride) (PDDAC) was introduced by Liu et al [34]. This can be coated on a capillary by simply flushing with a 0.1% solution to achieve a stable and reproducible reversed EOF [35].…”

Section: Introductionmentioning

confidence: 99%

Fluorinated ethylenepropylene copolymer as a potential capillary material in CE

et al. 2007

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In this work, a new generation UV-transparent polymer, fluorinated ethylenepropylene copolymer (FEP) exhibiting a low degree of crystallinity, extruded in dimensions similar to the most commonly used CE capillaries of approximately 80 mum id and about 360 mum od was investigated for its use as a CE capillary. FEP is transparent down to the low-UV region, and as fluorinated polymers in general are good electrical insulators and exhibit reasonable heat conductivity, it has considerable potential as a material for electrodriven analysis in capillary or microchip formats. The FEP capillary has been characterised with regard to some important aspects for its use as a CE capillary, including its profile of EOF versus pH, as well as procedures for manipulating EOF by coating the inner capillary wall with various semipermanent and dynamic layers. The FEP capillaries were tested and compared with fused-silica capillary for the separation of inorganic and small organic ions using conditions involving direct and indirect detection in the low-UV region. Finally, advantages of the use of the FEP capillary for simultaneous detection of a mixture containing nine inorganic cations and anions using indirect photometric detection with a movable light-emitting diode (LED) detector and a novel electrolyte are demonstrated.

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Poly(diallyldimethylammonium chloride) as a Cationic Coating for Capillary Electrophoresis

Cited by 59 publications

References 19 publications

A new and selective capillary column coated with cationic diazacrown ether for separation of organic and inorganic anions by capillary electrophoresis

A new and selective capillary column coated with cationic diazacrown ether for separation of organic and inorganic anions by capillary electrophoresis

An N‐methylpolyvinylpyridinium cationic polymer for capillary coating in electrophoresis of proteins and peptides

Fluorinated ethylenepropylene copolymer as a potential capillary material in CE

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