Poly(guanidinium ionic liquid)s particles as affinity platform for highly selective enrichment of phosphopeptides

Gu, Yalong; Guo, Yanling; Li, Haijie; Su, Rina; Sun, Kejin; Deng, Qiling; Wang, Shuo

doi:10.1039/c6ra00464d

Cited by 9 publications

(4 citation statements)

References 31 publications

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“…Ionic liquids (ILs), composed of cations and anions, have been widely studied for the applications in diversified areas such as catalytic synthesis, analytical chemistry, chemical process engineering, materials science, environmental science, and bioscience. − Although the number of ILs has been booming for over the past decades owing to the combination potential of innumerable cations and anions, the exploitation of ILs in phosphopeptide enrichment has been rarely reported. Traditional tetraalkylammonium- and alkyl guanidinium-modified materials have been synthesized and used for phosphopeptide enrichment. , …”

Section: Introductionmentioning

confidence: 99%

Phosphonate-Functionalized Ionic Liquid: A New Surface Modifier Contributing to the Enhanced Enrichment of Phosphorylated Peptides

Wang

Liang

et al. 2021

ACS Sustainable Chem. Eng.

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Phosphopeptide enrichment with high selectivity and detection sensitivity is essential for phosphoproteomic studies and remains a long-standing challenge. In this study, new immobilized metal affinity chromatography nanocomposite adsorbents with a phosphonate-functionalized ionic liquid (PFIL) as a surface modifier are successfully prepared via a reaction sequence of amination, quaternization, phosphonate hydrolyzation, and metal immobilization. Taking advantages of integrated features of a flexible and strong tripodal phosphonate chelator, a hydrophilic ionic liquid linker, a large surface area, and the size-exclusion effect, the resulting nanocomposite G@mSiO 2 -PFIL-Ti 4+ exhibits excellent detection sensitivity to enrich phosphorylated peptides from a tryptic β-casein digest (0.15 fmol), and superior enrichment selectivity to capture phosphorylated peptides from a digest mixture of β-casein and bovine serum albumin (a molar ratio of 1:10,000). Strong immobilization of tripodal chelation to metal ions endows the nanocomposite adsorbent with high tolerance to experimental conditions, and thus excellent reusability of the adsorbent has been achieved without remarkable loss of enrichment efficiency for 10 cycles. Due to the excellent size-exclusion effect, high enrichment specificity of G@mSiO 2 -PFIL-Ti 4+ to phosphopeptides has been observed and 23 endogenous phosphopeptides have been captured from human saliva. In addition, 924 phosphopeptides (enrichment specificity, 56.1%) have been identified from the tryptic digest of mouse brain lysate. Particularly, six of 975 phosphorylation sites were Alzheimer's disease-related hyperphosphorylation sites within tau protein. These results demonstrate that G@mSiO 2 -PFIL-Ti 4+ nanocomposite affinity materials show great application potential for a proteomic study of complicated biological samples.

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Section: Introductionmentioning

confidence: 99%

Phosphonate-Functionalized Ionic Liquid: A New Surface Modifier Contributing to the Enhanced Enrichment of Phosphorylated Peptides

Wang

Liang

et al. 2021

ACS Sustainable Chem. Eng.

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“…The observed excellent detection limit and the selectivity of G@mSiO 2 @PEI-PFIL-Ti 4+ toward phosphopeptides can be contributed to the combined unique feature of superhydrophilic flexible cationic linkers with highly dense phosphonate chelators and immobilized metal ions. 20,30 Adsorbent materials with application potential should have an excellent size-exclusion effect. The size-exclusion effect of the G@mSiO 2 @PEI-PFIL-Ti 4+ nanocomposite was explored by using three mixtures imitating real biological samples.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Superhydrophilic nanocomposite adsorbents modified via nitrogen-rich phosphonate-functionalized ionic liquid linkers: enhanced phosphopeptide enrichment and phosphoproteome analysis of tau phosphorylation in the hippocampal lysate of Alzheimer's transgenic mice

et al. 2022

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“… ,,− Compared with other guanidine-based technologies for analyzing phosphopeptides, the P5SOF matrix exhibited a more favorable enrichment performance (Table S3). − Furthermore, the reusability of mP5SOF-Arg was investigated to evaluate its performance as a reliable adsorbent. After finishing each MSPE procedure for phosphopeptide enrichment from 1 pmol μL –1 β-casein tryptic digest, mP5SOF-Arg was washed twice and then reused for the next run.…”

Section: Resultsmentioning

confidence: 99%

Engineering Magnetic Guanidyl-Functionalized Supramolecular Organic Framework for Efficient Enrichment of Global Phosphopeptides

Zheng

Zhang

et al. 2020

ACS Appl. Mater. Interfaces

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Comprehensive mass spectrometry-based proteomics analysis is currently available but remains challenging, especially for post-translational modifications of phosphorylated proteins. Herein, multifunctional magnetic pillar[5]arene supramolecular organic frameworks were fabricated and immobilized with arginine (mP5SOF-Arg) for highly effective enrichment of global phosphopeptides. The specific phosphate-P5/ phosphate-guanidine affinities and large surface area with regular porosity contribute to the high enrichment capacity. By coupling with mass spectrometry, high detection sensitivity (0.1 fmol), excellent selectivity (1:5000 molar ratios of β-casein/cytochrome c), and high recyclability (seven cycles) were achieved for phosphopeptide analysis. mP5SOF-Arg can efficiently enrich phosphopeptides from practical samples, including defatted milk, egg yolk, and human saliva. Notably, a total of 450 phosphopeptides were explored for highly selective identification from A594 cells and 1445 phosphopeptides were identified from mouse liver tissue samples. mP5SOF-Arg exhibited great potential to serve as the basis for peptidomic research to identify phosphopeptides and provided insight for biomarker discovery.

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Poly(guanidinium ionic liquid)s particles as affinity platform for highly selective enrichment of phosphopeptides

Abstract: Is it feasible or not that the poly(guanidinium ionic liquid)s material are employed to enrich phosphopeptides?

Cited by 9 publications

References 31 publications

Phosphonate-Functionalized Ionic Liquid: A New Surface Modifier Contributing to the Enhanced Enrichment of Phosphorylated Peptides

Phosphonate-Functionalized Ionic Liquid: A New Surface Modifier Contributing to the Enhanced Enrichment of Phosphorylated Peptides

Superhydrophilic nanocomposite adsorbents modified via nitrogen-rich phosphonate-functionalized ionic liquid linkers: enhanced phosphopeptide enrichment and phosphoproteome analysis of tau phosphorylation in the hippocampal lysate of Alzheimer's transgenic mice

Engineering Magnetic Guanidyl-Functionalized Supramolecular Organic Framework for Efficient Enrichment of Global Phosphopeptides

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