Poly(N-isopropylacrylamide) modified polydopamine as a temperature-responsive surface for cultivation and harvest of mesenchymal stem cells

Zhang, Jun; Peng, Ching‐An

doi:10.1039/c7bm00371d

Cited by 23 publications

(20 citation statements)

References 38 publications

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“…[14][15][16][17] PNIPAM is a temperature-responsive polymer that is biocompatible and has been extensively used for producing microgels, biosensors, and in drug delivery applications. [18][19][20][21][22][23] PNIPAM has a low critical solution temperature (LCST) of ≈32 °C. [24] Above this temperature, the polymer is hydrophobic and the polymeric chains form a globular and packed conformation.…”

Section: Introductionmentioning

confidence: 99%

Multifunctional Thermoresponsive Microcarriers for High‐Throughput Cell Culture and Enzyme‐Free Cell Harvesting

Dabiri

Samiei

Shojaei

et al. 2021

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An effective treatment of human diseases using regenerative medicine and cell therapy approaches requires a large number of cells. Cultivation of cells on microcarriers is a promising approach due to the high surface‐to‐volume ratios that these microcarriers offer. Here, multifunctional temperature‐responsive microcarriers (cytoGel) made of an interpenetrating hydrogel network composed of poly(N‐isopropylacrylamide) (PNIPAM), poly(ethylene glycol) diacrylate (PEGDA), and gelatin methacryloyl (GelMA) are developed. A flow‐focusing microfluidic chip is used to produce microcarriers with diameters in the range of 100–300 μm and uniform size distribution (polydispersity index of ≈0.08). The mechanical properties and cells adhesion properties of cytoGel are adjusted by changing the composition hydrogel composition. Notably, GelMA regulates the temperature response and enhances microcarrier stiffness. Human‐derived glioma cells (U87) are grown on cytoGel in static and dynamic culture conditions with cell viabilities greater than 90%. Enzyme‐free cell detachment is achieved at room temperature with up to 70% detachment efficiency. Controlled release of bioactive molecules from cytoGel is accomplished for over a week to showcase the potential use of microcarriers for localized delivery of growth factors to cell surfaces. These microcarriers hold great promise for the efficient expansion of cells for the industrial‐scale culture of therapeutic cells.

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Section: Introductionmentioning

confidence: 99%

Multifunctional Thermoresponsive Microcarriers for High‐Throughput Cell Culture and Enzyme‐Free Cell Harvesting

Dabiri

Samiei

Shojaei

et al. 2021

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“…Therefore, the changing trend of D H along with temperature is usually employed to infer the temperature sensitivity of PNIPAAm-containing materials. Given that the overall structure and surface property of the end products can be changed by temperature control with appropriate design and simplified operation, PNIPAAm and its derivatives are widely used for drug delivery [ 2 ], biomacromolecule immobilization [ 3 ], intelligent cell culture substrate [ 4 ], artificial extracellular matrix [ 5 ] and other biomedical fields. Phenylboronic acid (PBA) can ionize in aqueous solutions to give uncharged hydrophobic forms and charged hydrophilic forms.…”

Section: Introductionmentioning

confidence: 99%

Multi-Responsive Behaviors of Copolymers Bearing N-Isopropylacrylamide with or without Phenylboronic Acid in Aqueous Solution

Yang

Fan

et al. 2018

Polymers

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Continuing efforts to develop novel smart materials are anticipated to upgrade the quality of life of humans. Thermo-responsive poly(N-isopropylacrylamide) and glucose-responsive phenylboronic acid-typical representatives-are often integrated as multi-stimuli-sensitive materials, but few are available for side-by-side comparisons with their properties. In this study, both copolymers bearing N-isopropylacrylamide (NIPAAm), with or without 3-acrylamidophenylboronic acid (AAPBA), were synthesized by free radical polymerization, and characterized by Fourier transform infrared spectrometry, nuclear magnetic resonance hydrogen spectroscopy and gel permeation chromatography. Dynamic light scattering was used to analyze and compare the responsive behaviors of the copolymers in different aqueous solutions. Atomic force microscopy was also employed to investigate the apparent morphology changes with particle sizes. The results demonstrated that the introduction of NIPAAm endowed the composite materials with thermosensitivity, whereas the addition of AAPBA lowered the molecular weight of the copolymers, intensified the intermolecular aggregation of the nanoparticles, reduced the lower critical solution temperature (LCST) of the composites, and accordingly allowed the copolymers to respond to glucose. It was also concluded that the responding of smart copolymers to operating parameters can be activated only under special conditions, and copolymer dimension and conformation were affected by inter/intramolecular interactions.

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“…As illustrated in Table , the total interface free energy (Δ G ) of the thermoresponsive surface, microalgae cells, and liquid from −39.44 mJ m –2 at 15 °C changed to −71.93 mJ m –2 at 35 °C. The negative Δ G means the process would happen and the more negative the value, the greater the probability of occurrence. , Therefore, a decreased Δ G means that the adhesion energy of the TMRS to C. vulgaris cells increased by 135.6% when the temperature rose from 15 to 35 °C. Additionally, the LW component of the Δ G was −2.75 mJ m –2 at 15 °C and −3.99 mJ m –2 at 35 °C, while the AB component of the Δ G changes from −36.69 mJ m –2 at 15 °C to −67.94 mJ m –2 at 35 °C.…”

Section: Results and Discussionmentioning

confidence: 99%

Thermoresponsive Surfaces Grafted by Shrinkable Hydrogel Poly(N-isopropylacrylamide) for Controlling Microalgae Cells Adhesion during Biofilm Cultivation

Zeng

Huang

Xia

et al. 2021

Environ. Sci. Technol.

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Microalgae is a promising candidate for reducing greenhouse gas and producing renewable biofuels. For microalgae biofilm cultivation, a strong adhesion ability of microalgae cells onto the surface is a prerequisite to resist the fluid shear stress, while strong adhesion is not of benefit to the biofilm harvesting process. To solve this dilemma, a thermoresponsive surface (TMRS) with lower critical solution temperature of 33 °C was made by grafting N-isopropylacrylamide onto a silicate glass slide. The wettability of the TMRS changed from hydrophilic (contact angle of 59.4°) to hydrophobic (contact angle of 91.6°) when the temperature rose from 15 to 35 °C, resulting in the increase of adhesion energy of the TMRS to Chlorella vulgaris cells by 135.6%. The experiments showed that the cells were more likely to attach onto the TMRS at the higher temperature of 35 °C owing to the surface microstructures generated by the hydrogel layer shrinkage, which is similar in size to the microalgae cells. And the cell coverage rate on TMRS increased by 32% compared to the original glass surface. Conversely, the cells separate easily from the TMRS at a lower temperature of 15 °C, and the cell adhesion density was reduced by 19% due to hydrogel layer swelling to a relatively flat surface.

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Poly(N-isopropylacrylamide) modified polydopamine as a temperature-responsive surface for cultivation and harvest of mesenchymal stem cells

Cited by 23 publications

References 38 publications

Multifunctional Thermoresponsive Microcarriers for High‐Throughput Cell Culture and Enzyme‐Free Cell Harvesting

Multifunctional Thermoresponsive Microcarriers for High‐Throughput Cell Culture and Enzyme‐Free Cell Harvesting

Multi-Responsive Behaviors of Copolymers Bearing N-Isopropylacrylamide with or without Phenylboronic Acid in Aqueous Solution

Thermoresponsive Surfaces Grafted by Shrinkable Hydrogel Poly(N-isopropylacrylamide) for Controlling Microalgae Cells Adhesion during Biofilm Cultivation

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