Polyacrylamide gels of increasing concentration gradient for the electrophoresis of lipoproteins

Low‐density‐lipoprotein peak particle size in a Mediterranean population

“…Migration distance for each absorbance peak was determined and the molecular diameter corresponding to each peak was calculated from a calibration curve generated from the migration distance of size standards of known diameters, which included lipoprotein calibrators of previously determined particle sizes (kindly provided by R. M. Krauss and P. J. Blanche from the Lawrence Berkeley National Laboratory, University of California, Berkeley, CA, USA), as well as carboxylated latex beads (Duke Scientific, Palo Alto, CA), thyroglobulin and apoferritin (HMW Std, Pharmacia, Piscataway, NJ) having molecular diameters of 380 Å, 170 Å and 122 Å, respectively. Twenty randomized samples were also tested in a reference laboratory (Lawrence Berkeley National Laboratory, Berkeley, CA, USA), using gels made by established procedures [28,29], without significant differences in the results. LDL phenotype A was defined as an LDL subclass pattern with the major gradient gel peak at a particle diameter of 258·4 Å or greater, whereas the major peak of LDL phenotype B was at a particle diameter of less than 258·4 Å[26].…”

Type III Hyperlipoproteinaemia

“…One patient had a father with gout, and this patient was the only one in the series who suffered from gout, in spite of the generally high serum uric acid values (Table IV'. Lipoprotein electrophoresis was performed in 12 cases on polyacrylamide gel, by the method of Pratt and Dangerfield (1969). In this method the very low density lipoproteins, corresponding to the prebetalipoprotein band in paper electrophoresis, do not migrate as far as the low density lipoproteins (betalipoprotein band) and are therefore found behind and not in front (postbeta and not prebeta).…”

Plasma Lipids and Lipoproteins

“…The use of polyacrylamide, either in linear (Narayan et al, 1966) or gradient (pratt and Dangerfield, 1969) gels, provides excellent separation of a multiplicity of discrete bands which do not, at our present level of knowledge, seem more clinically informative than simpler methods. Probably, a laboratory intending to provide lipoprotein analysis should choose at present between cellulose acetate and agarose.…”