Polydopamine-mediated surface modification of scaffold materials for human neural stem cell engineering

Yang, Kisuk; Lee, Jung Seung; Kim, Jin; Lee, Yu Bin; Shin, Heungsoo; Um, Soong Ho; Kim, Jeong Beom; Park, Kook In; Lee, Haeshin; Cho, Seung Woo

doi:10.1016/j.biomaterials.2012.06.067

Cited by 322 publications

(298 citation statements)

References 39 publications

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“…28 PDA coating displays excellent biocompatibility and low cytotoxicity, making it a versatile platform for applications, including biomolecule immobilization, molecular surface imprinting for protein recognition, cells adhesion, and encapsulation. [29][30][31] Furthermore, PDA is also a major pigment of naturally occurring eumelanin. 32 PDA displays a lot of striking properties of naturally occurring eumelanin in terms of electrical, optical, and magnetic properties.…”

Section: Introductionmentioning

confidence: 99%

Surface modification of MPEG-b-PCL-based nanoparticles via oxidative self-polymerization of dopamine for malignant melanoma therapy

Xiong¹,

Peng²,

Chen³

et al. 2015

IJN

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To enhance the therapeutic effects of chemotherapy on malignant melanoma, paclitaxel (PTX)-loaded methoxy poly(ethylene glycol)- b -poly(ε-caprolactone) nanoparticles (MPEG- b -PCL NPs) that had their surfaces modified with polydopamine (PTX-loaded MPEG- b -PCL NPs@PDA) were prepared as drug vehicles. The block copolymer MPEG- b -PCL was synthesized by ring-opening polymerization and characterized by proton nuclear magnetic resonance spectroscopy and gel permeation chromatography. The PTX-loaded NPs were prepared by a modified nanoprecipitation technique. The PTX-loaded NPs and PTX-loaded NPs@PDA were characterized in terms of size and size distribution, zeta potential, surface morphology, drug encapsulation efficiency, and drug release. Confocal laser scanning microscopy showed that coumarin-6-loaded NPs@PDA could be internalized by human melanoma cell line A875 cells. The cellular uptake efficiency of NPs was greatly enhanced after PDA modification. The antitumor efficacy of the PTX-loaded NPs@PDA was investigated in vitro by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and in vivo by a xenograft tumor model. The PTX-loaded NPs@PDA could significantly inhibit tumor growth compared to Taxol ® and precursor PTX-loaded NPs. All the results suggested that the PTX-loaded MPEG- b -PCL NPs that had their surfaces modified with PDA are promising nanocarriers for malignant melanoma therapy.

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Section: Introductionmentioning

confidence: 99%

Surface modification of MPEG-b-PCL-based nanoparticles via oxidative self-polymerization of dopamine for malignant melanoma therapy

Xiong¹,

Peng²,

Chen³

et al. 2015

IJN

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“…Typical chemical conjugation methods, on the other hand, require complicated and multi-step procedures such as surface activation or functionalization steps requiring plasma or chemical treatments [23]. Nevertheless, denaturation of bioactive agents by toxic chemicals and multiple reaction steps may restrict their applications in clinic [23,24]. Therefore, there is a pressing need to develop a non-toxic, facile and effective implant modification method for prevention of infections on the implant interface.…”

Section: Introductionmentioning

confidence: 99%

“…Covalent bonding of biocompatible antibacterial agents onto the implant interface appears promising, because agents will not be released, and efficacy can be maintained for long-term application [22]. Typical chemical conjugation methods, on the other hand, require complicated and multi-step procedures such as surface activation or functionalization steps requiring plasma or chemical treatments [23]. Nevertheless, denaturation of bioactive agents by toxic chemicals and multiple reaction steps may restrict their applications in clinic [23,24].…”

Section: Introductionmentioning

confidence: 99%

Antibiotic-decorated titanium with enhanced antibacterial activity through adhesive polydopamine for dental/bone implant

Zhou

Wang

et al. 2014

J. R. Soc. Interface.

121

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Implant-associated infections, which are normally induced by microbial adhesion and subsequent biofilm formation, are a major cause of morbidity and mortality. Therefore, practical approaches to prevent implant-associated infections are in great demand. Inspired by adhesive proteins in mussels, here we have developed a novel antibiotic-decorated titanium (Ti) material with enhanced antibacterial activity. In this study, Ti substrate was coated by one-step pH-induced polymerization of dopamine followed by immobilization of the antibiotic cefotaxime sodium (CS) onto the polydopamine-coated Ti through catechol chemistry. Contact angle measurement and X-ray photoelectron spectroscopy confirmed the presence of CS grafted on the Ti surface. Our results demonstrated that the antibiotic-grafted Ti substrate showed good biocompatibility and well-behaved haemocompatibility. In addition, the antibiotic-grafted Ti could effectively prevent adhesion and proliferation of Escherichia coli (Gram-negative) and Streptococcus mutans (Gram-positive). Moreover, the inhibition of biofilm formation on the antibiotic-decorated Ti indicated that the grafted CS could maintain its long-term antibacterial activity. This modified Ti substrate with enhanced antibacterial activity holds great potential as implant material for applications in dental and bone graft substitutes.

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“…The pDA coating alone on PLGA substrates may enhance neuronal differentiation of human NSCs. 41 ECM proteins serve as nonsoluble cues to modulate cell fate through cell signaling cascades. COL I and fibronectin stimulate migration and proliferation of osteoblasts by direct binding to cellular transmembrane receptors.…”

Section: Cell Proliferation Analysismentioning

confidence: 99%

“…In a previous study, the contact angle of PLGA thin film, a similar hydrophilic surface, decreased after pDA functionalization from a starting angle of 68.6°±3.8° to 27.3°±6.8°, indicating that the polymer surface is proved to be more hydrophilic after pDA coating. 41 Furthermore, attributed to the achieved modification of COL I with superior hydrophilicity mediated by pDA coatings onto the surface of PLGA/PCL interconnected nanofibers, the contact angle of COL I-pDA-PLGA/ PCL ES dramatically descended to 5.0°±1.2°. There was a significant difference in hydrophilicity in the PLGA/PCL ES membrane before and after modification by pDA coatings (P,0.05) or immobilization of bioactive molecules mediated by pDA coatings (P,0.05), which may provide the more appropriate surface wettability for attachment and proliferation of cells such as endothelial cells, neural stem cells (NSCs), and mesenchymal stem cells (MSCs) reported in several studies.…”

mentioning

confidence: 99%

The preosteoblast response of electrospinning PLGA/PCL nanofibers: effects of biomimetic architecture and collagen I

Qian¹,

Chen²,

Yang³

et al. 2016

IJN

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Constructing biomimetic structure and incorporating bioactive molecules is an effective strategy to achieve a more favorable cell response. To explore the effect of electrospinning (ES) nanofibrous architecture and collagen I (COL I)-incorporated modification on tuning osteoblast response, a resorbable membrane composed of poly(lactic-co-glycolic acid)/poly(caprolactone) (PLGA/PCL; 7:3 w/w) was developed via ES. COL I was blended into PLGA/PCL solution to prepare composite ES membrane. Notably, relatively better cell response was delivered by the bioactive ES-based membrane which was fabricated by modification of 3,4-dihydroxyphenylalanine and COL I. After investigation by field emission scanning electron microscopy, Fourier transform infrared spectroscopy, contact angle measurement, and mechanical test, polyporous three-dimensional nanofibrous structure with low tensile force and the successful integration of COL I was obtained by the ES method. Compared with traditional PLGA/PCL membrane, the surface hydrophilicity of collagen-incorporated membranes was largely enhanced. The behavior of mouse preosteoblast MC3T3-E1 cell infiltration and proliferation on membranes was studied at 24 and 48 hours. The negative control was fabricated by solvent casting. Evaluation of cell adhesion and morphology demonstrated that all the ES membranes were more favorable for promoting the cell adhesion and spreading than the casting membrane. Cell Counting Kit-8 assays revealed that biomimetic architecture, surface topography, and bioactive properties of membranes were favorable for cell growth. Analysis of β1 integrin expression level by immunofluorescence indicated that such biomimetic architecture, especially COL I-grafted surface, plays a key role in cell adhesion and proliferation. The real-time polymerase chain reaction suggested that both surface topography and bioactive properties could facilitate the cell adhesion. The combined effect of biomimetic architecture with enhanced surface activity by 3,4-dihydroxyphenylalanine-assisted modification and COL I incorporation of PLGA/PCL electrospun membranes could successfully fill osteogenic defects and allow for better cell proliferation and differentiation.

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Polydopamine-mediated surface modification of scaffold materials for human neural stem cell engineering

Cited by 322 publications

References 39 publications

Surface modification of MPEG-b-PCL-based nanoparticles via oxidative self-polymerization of dopamine for malignant melanoma therapy

Surface modification of MPEG-b-PCL-based nanoparticles via oxidative self-polymerization of dopamine for malignant melanoma therapy

Antibiotic-decorated titanium with enhanced antibacterial activity through adhesive polydopamine for dental/bone implant

The preosteoblast response of electrospinning PLGA/PCL nanofibers: effects of biomimetic architecture and collagen I

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