Polyelectrolyte Multilayer Films Modification with Ag and rGO Influences Platelets Activation and Aggregate Formation under In Vitro Blood Flow

Imbir, Gabriela; Mzyk, Aldona; Trembecka-Wójciga, Klaudia; Jasek-Gajda, Ewa; Plutecka, Hanna; Schirhagl, Romana; Major, R.

doi:10.3390/nano10050859

Cited by 8 publications

(3 citation statements)

References 37 publications

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“…This finding is consistent with other reports using carbodiimide chemistry for PEMs presenting an increasing hydrophilicity upon cross-linking . More hydrophilic surfaces after cross-linking have been previously explained by the formation of new cross-linker conjugates and denser structures between the polyelectrolytes as well as an enhanced roughness. − …”

Section: Results and Discussionsupporting

confidence: 92%

Engineering of Stable Cross-Linked Multilayers Based on Thermo-Responsive PNIPAM-Grafted-Chitosan/Heparin to Tailor Their Physiochemical Properties and Biocompatibility

Zeng

Fuhrmann

et al. 2022

ACS Appl. Mater. Interfaces

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The thermo-responsive poly(N-isopropylacrylamide) (PNIPAM) is ubiquitously applied in controlled drug release and tissue engineering. However, the lack of bioactivity of PNIPAM restricts its use in cell-containing systems being a thermo-responsive adhesive substratum with no regulating effect on cell growth and differentiation. In this study, integrating PNIPAM with chitosan into PNIPAM-grafted-chitosan (PNIPAM–Chi) allows a layer-by-layer assembly with bioactive heparin to fabricate PNIPAM-modified polyelectrolyte multilayers (PNIPAM–PEMs). Grafting PNIPAM chains of either 2 (LMW) or 10 kDa (HMW) on the chitosan backbone influences the cloud point (CP) temperature in the range from 31 to 33 °C. PNIPAM–Chi with either a higher molecular weight or a higher degree of substitution of PNIPAM chains exhibiting a significant increase in diameter above CP as ensured by dynamic light scattering is selected to fabricate PEM with heparin as a polyanion at pH 4. Little difference of layer growth is detected between the chosen PNIPAM–Chi used as polycations by surface plasmon resonance, while multilayers formed with HMW-0.02 are more hydrated and show striking swelling-and-shrinking abilities when studied with quartz crystal microbalance with dissipation monitoring. Subsequently, the multilayers are covalently cross-linked using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide to strengthen the stability of the systems under physiological conditions. Ellipsometry results confirm the layer integrity after exposure to the physiological buffer at pH 7.4 compared to those without cross-linking. Moreover, significantly higher adhesion and more spreading of C3H10T1/2 multipotent embryonic mouse fibroblasts on cross-linked PEMs, particularly with heparin terminal layers, are observed owing to the bioactivity of heparin. The slightly more hydrophobic surfaces of cross-linked PNIPAM–PEMs at 37 °C also increase cell attachment and growth. Thus, layer-by-layer constructed PNIPAM–PEM with cross-linking represents an interesting cell culture system that can be potentially employed for thermally uploading and controlled release of growth factors that further promotes tissue regeneration.

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Section: Results and Discussionsupporting

confidence: 92%

Engineering of Stable Cross-Linked Multilayers Based on Thermo-Responsive PNIPAM-Grafted-Chitosan/Heparin to Tailor Their Physiochemical Properties and Biocompatibility

Zeng

Fuhrmann

et al. 2022

ACS Appl. Mater. Interfaces

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“…In turn, attaining these properties would aid in applications such as drug encapsulation, where the loading capacity can further increase [ 11 ]. In other studies, frequently used crosslinking techniques such as incorporating N-hydroxysulphosuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) into PEM films have further shown modifications in surface roughness, wettability and stiffness, which are applicable to gels [ 84 ]. Taking stiffness as an example, multilayered structures were shown to increase up to 10-fold due to the crosslinked network obtained [ 85 ].…”

Section: Fabrication Of Different Types Of Pe Gelsmentioning

confidence: 99%

Polyelectrolyte Gels: Fundamentals, Fabrication and Applications

Wanasingha¹,

Dorishetty²,

Dutta³

et al. 2021

Gels

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Polyelectrolyte gels are an important class of polymer gels and a versatile platform with charged polymer networks with ionisable groups. They have drawn significant recent attention as a class of smart material and have demonstrated potential for a variety of applications. This review begins with the fundamentals of polyelectrolyte gels, which encompass various classifications (i.e., origin, charge, shape) and crucial aspects (ionic conductivity and stimuli responsiveness). It further centralises recent developments of polyelectrolyte gels, emphasising their synthesis, structure–property relationships and responsive properties. Sequentially, this review demonstrates how polyelectrolyte gels’ flourishing properties create attractiveness to a range of applications including tissue engineering, drug delivery, actuators and bioelectronics. Finally, the review outlines the indisputable appeal, further improvements and emerging trends in polyelectrolyte gels.

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“…The polymers need to have mutual attractive forces such as ionic forces, van der Waals forces, or hydrogen bonding. − In the human body, five types of GAGs can be found: heparan sulfate, chondroitin sulfate (CS), dermatan sulfate, keratan sulfate, and hyaluronic acid (HA) . Mostly heparin, which is similar to heparin sulfate, HA, and CS-based coatings can be found in the literature. ,− Here not only the composition of the PEMs is varied but also the surface roughness . Hedayati et al (2018) showed that the addition of topographical features decreases the protein adsorption as well as the fibrin formation significantly .…”

Section: Recent Efforts To Reduce Platelet-surface Activationmentioning

confidence: 99%

Modulation of Platelet-Surface Activation: Current State and Future Perspectives

Apte

Börke

Rothe

et al. 2020

ACS Appl. Bio Mater.

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Modulation of platelet-surface activation is important for many biomedical applications such as in vivo performance, platelet storage, and acceptance of an implant. Reducing platelet-surface activation is challenging because they become activated immediately after short contact with nonphysiological surfaces. To date, controversies and open questions in the field of platelet-surface activation still remain. Here, we review state-of-the-art approaches in inhibiting platelet-surface activation, mainly focusing on modification, patterning, and methodologies for characterization of the surfaces. As a future perspective, we discuss how the combination of biochemical and physiochemical strategies together with the topographical modulations would assist in the search for an ideal nonthrombogenic surface.

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Polyelectrolyte Multilayer Films Modification with Ag and rGO Influences Platelets Activation and Aggregate Formation under In Vitro Blood Flow

Cited by 8 publications

References 37 publications

Engineering of Stable Cross-Linked Multilayers Based on Thermo-Responsive PNIPAM-Grafted-Chitosan/Heparin to Tailor Their Physiochemical Properties and Biocompatibility

Engineering of Stable Cross-Linked Multilayers Based on Thermo-Responsive PNIPAM-Grafted-Chitosan/Heparin to Tailor Their Physiochemical Properties and Biocompatibility

Polyelectrolyte Gels: Fundamentals, Fabrication and Applications

Modulation of Platelet-Surface Activation: Current State and Future Perspectives

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