Polyelectrolyte Multilayers in Microfluidic Systems for Biological Applications

Minnikanti, Saugandhika; Gangopadhyay, Aveek; Reyes, Darwin R.

doi:10.3390/polym6082100

Cited by 10 publications

(5 citation statements)

References 51 publications

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“…Gradient materials along X and Z directions have also been produced by LbL methodology to rapidly screen physicochemical properties and cell‐biomaterial interactions . To achieve this propose some studies combine LbL assembly with microfluidic approaches . The combination of both techniques allow to control the buildup in the plane of the substrate, easy combination of multiple polyelectrolytes, fine control over the flow, short diffusion times in microchannels, use of small volume, large surface to volume ratio and a laminar flow …”

Section: Introductionmentioning

confidence: 99%

Biomimetic Extracellular Environment Based on Natural Origin Polyelectrolyte Multilayers

Silva

Reis

Mano

2016

Small

109

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Surface modification of biomaterials is a well-known approach to enable an adequate biointerface between the implant and the surrounding tissue, dictating the initial acceptance or rejection of the implantable device. Since its discovery in early 1990s layer-by-layer (LbL) approaches have become a popular and attractive technique to functionalize the biomaterials surface and also engineering various types of objects such as capsules, hollow tubes, and freestanding membranes in a controllable and versatile manner. Such versatility enables the incorporation of different nanostructured building blocks, including natural biopolymers, which appear as promising biomimetic multilayered systems due to their similarity to human tissues. In this review, the potential of natural origin polymer-based multilayers is highlighted in hopes of a better understanding of the mechanisms behind its use as building blocks of LbL assembly. A deep overview on the recent progresses achieved in the design, fabrication, and applications of natural origin multilayered films is provided. Such films may lead to novel biomimetic approaches for various biomedical applications, such as tissue engineering, regenerative medicine, implantable devices, cell-based biosensors, diagnostic systems, and basic cell biology.

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Section: Introductionmentioning

confidence: 99%

Biomimetic Extracellular Environment Based on Natural Origin Polyelectrolyte Multilayers

Silva

Reis

Mano

2016

Small

109

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“…The deposition is hereby solely dependent on the Brownian diffusion to the bottom layer of the multilayer. Through the utilization of a material-efficient fluidic-assisted stop-flow multilayer deposition method, we achieved a more economical and time-efficient procedure, eliminating the need for a washing step. , Due to the geometry of the immobilization channel, the (PPC/PAA) multilayer assembly was completed within only 8 min. To assess the biofunctionality of pSA when inside the PPC/PAA multilayer lane, the binding efficiency of a biotinylated Rhodamine 6G (Rh-6G, λ ex = 525 nm, λ em = 548 nm) to pSA within the multilayer was investigated.…”

Section: Resultsmentioning

confidence: 99%

Membrane-Free Lateral Flow Assay with the Active Control of Fluid Transport for Ultrasensitive Cardiac Biomarker Detection

Strohmaier-Nguyen,

Horn,

Baeumner

2024

Anal. Chem.

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Membrane-based lateral flow immunoassays (LFAs) have been employed as early point-of-care (POC) testing tools in clinical settings. However, the varying membrane properties, uncontrollable sample transport in LFAs, visual readout, and required large sample volumes have been major limiting factors in realizing needed sensitivity and desirable precise quantification. Addressing these challenges, we designed a membrane-free system in which the desirable three-dimensional (3D) structure of the detection zone is imitated and used a small pump for fluid flow and fluorescence as readout, all the while maintaining a one-step assay protocol. A hydrogel-like protein−polyelectrolyte complex (PPC) within a polyelectrolyte multilayer (PEM) was developed as the test line by complexing polystreptavidin (pSA) with poly-(diallyldimethylammonium chloride) (PDDA), which in turn was layered with poly(acrylic acid) (PAA) resulting in a superior 3D streptavidin-rich test line. Since the remainder of the microchannel remains material-free, good flow control is achieved, and with the total volume of 20 μL, 7.5-fold smaller sample volumes can be used in comparison to conventional LFAs. High sensitivity with desirable reproducibility and a 20 min total assay time were achieved for the detection of NT-proBNP in plasma with a dynamic range of 60−9000 pg•mL −1 and a limit of detection of 56 pg•mL −1 using probe antibody-modified fluorescence nanoparticles. While instrument-free visual detection is no longer possible, the developed lateral flow channel platform has the potential to dramatically expand the LFA applicability, as it overcomes the limitations of membrane-based immunoassays, ultimately improving the accuracy and reducing the sample volume so that finger-prick analyses can easily be done in a one-step assay for analytes present at very low concentrations.

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“…A method that has been used for the formation of 3D scaffolds based on PEMCs is microfluidics. A fluidic assembly can be used for the formation of a thin film via coating the substrates inside a microchannel [ 126 , 222 ]. In terms of the microfluidic assembly, the procedure of the multilayer deposition occurs using fluidic equipment such as fluidic micro-channel for the coating of the substrate, tubes or capillaries for the induction/pull of polymer and washing solutions into microchannel, and a pump (or vacuum) that induces pressure to the system for the sequential movement of polymer and washing solutions through the microchannel.…”

Section: Pemc-based Scaffoldsmentioning

confidence: 99%

Polyelectrolyte Multilayer Capsule (PEMC)-Based Scaffolds for Tissue Engineering

et al. 2020

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Tissue engineering (TE) is a highly multidisciplinary field that focuses on novel regenerative treatments and seeks to tackle problems relating to tissue growth both in vitro and in vivo. These issues currently involve the replacement and regeneration of defective tissues, as well as drug testing and other related bioapplications. The key approach in TE is to employ artificial structures (scaffolds) to support tissue development; these constructs should be capable of hosting, protecting and releasing bioactives that guide cellular behaviour. A straightforward approach to integrating bioactives into the scaffolds is discussed utilising polyelectrolyte multilayer capsules (PEMCs). Herein, this review illustrates the recent progress in the use of CaCO3 vaterite-templated PEMCs for the fabrication of functional scaffolds for TE applications, including bone TE as one of the main targets of PEMCs. Approaches for PEMC integration into scaffolds is addressed, taking into account the formulation, advantages, and disadvantages of such PEMCs, together with future perspectives of such architectures.

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Polyelectrolyte Multilayers in Microfluidic Systems for Biological Applications

Cited by 10 publications

References 51 publications

Biomimetic Extracellular Environment Based on Natural Origin Polyelectrolyte Multilayers

Biomimetic Extracellular Environment Based on Natural Origin Polyelectrolyte Multilayers

Membrane-Free Lateral Flow Assay with the Active Control of Fluid Transport for Ultrasensitive Cardiac Biomarker Detection

Polyelectrolyte Multilayer Capsule (PEMC)-Based Scaffolds for Tissue Engineering

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