Polyethylenimine Functionalized Ultrasmall Mesoporous Silica Nanoparticles for siRNA Delivery

Abstract

Small interfering RNA (siRNA) for gene therapy has attracted great attention. However, due to easy degradation and inaccessibility to the cells, the gene silencing efficiency is not always satisfactory. To overcome this hurdle, developing an excellent nanocarrier to deliver siRNA into cancer cells is crucial. Fluorescent mesoporous silica nanoparticles are considered to be a superb nanocarrier for siRNA delivery systems because of its negligible toxicity and good biocompatibility. Herein, we developed an efficient fluorescent ultrasmall mesoporous silica nanocarrier for siRNA loading and delivery. The ultrasmall nanocarriers with polyethyleni-mine coating have a uniform size of about 10 nm. We find that the amino groups on polyethylenimine can protect siRNA from being degraded. Based on the fluorescence observation, we show that polyethylenimine-coated mesoporous silica nanocarrier loaded with siRNA can be uptake by 293T cells. Importantly, we also prove that siRNA can be delivered into 293T cells by our nanocarrier and subsequently inhibit the expression of related genes. The study provides insights into the development of nonviral fluorescence nanocarriers for siRNA delivery, which have important implications for targeted cancer therapy.

Delivery of Therapeutic Biopolymers Employing Silica-Based Nanosystems

“…This effect, although not detailed in depth, could be a consequence of the better hosting and protection of plasmids at the larger mesopores. In another contribution, Su, Deng and coworkers, studied the performance of ultrasmall MSNs for siRNA delivery employing PEI too [ 51 ]. Their nanosystem, with an overall size of less than 12 nm, showed good biocompatibility on cancerous HeLa cultures (up to 200 μg·mL −1 ) and up to 80% knockdown onto glyceraldehyde-3-phosphate dehydrogenase (GADPH) onto HEK-293T cells, suggesting a great transfection efficiency similar to the reference, Lipofectamine 2000.…”