Polymer-coated cardiopulmonary bypass circuit attenuates upregulation of both proteases/protease inhibitors and platelet degranulation in pigs

Suehiro, S; Shimizu, Katsuyoshi; Imai, Kensuke; Niii, Atsushi; Akeho, Kazuhiro; Nakata, Hayato; Yamaguchi, Akane; Matsumoto, K.; Oda, Teiji

doi:10.1177/0267659117715506

Cited by 6 publications

(12 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They demonstrated that the SEC-coated circuit has biocompatibility equivalent to that of the PMEA-coated circuit, with no significant differences in coagulation or inflamma-tion during CPB. Suehiro et al 4 demonstrated in a pig model of CPB that the SEC-coated circuit effectively attenuated the upregulation of proteins related to platelet degranulation. This study showed that the heparin dose and subsequent protamine dose were significantly higher in the SEC-coated circuit than those in the heparin-coated circuit as will be understood.…”

Section: Discussionmentioning

confidence: 99%

Clinical application of a new ternary polymer, SEC-1 coat™, for pediatric cardiopulmonary bypass circuits: a prospective randomized pilot study

et al. 2020

View full text Add to dashboard Cite

Objective: The use of biocompatible materials to reduce the systemic activation of inflammation and coagulation pathways is expanding rapidly. However, there have been few clinical studies of biocompatible circuits for pediatric cardiopulmonary bypass. This pilot study aimed to preliminarily evaluate the biocompatibility of SEC-1 coat™ (SEC) for cardiopulmonary bypass circuits in pediatric cardiac surgery. Methods: Twenty infants undergoing cardiac surgery for isolated ventricular septal defects at Kobe Children’s Hospital were assigned randomly to an SEC-coated (SEC group, n = 10) or heparin-coated (control group, n = 10) circuit. Perioperative data and the following markers were prospectively analyzed: platelet counts and interleukin-6, interleukin-8, C3a, β-thromboglobulin, and thrombin–antithrombin complex levels. Results: Neither patient characteristics nor postoperative clinical outcomes differed significantly between the SEC and control groups. Platelet counts markedly decreased during cardiopulmonary bypass in both groups, but were significantly better preserved in the SEC group. Fewer patients needed postoperative platelet transfusions in the SEC group. After cardiopulmonary bypass termination, serum levels of β-thromboglobulin and thrombin–antithrombin complex were significantly lower in the SEC than in the control group. Although the differences were not statistically significant, serum levels of interleukin-6, interleukin-8, and C3a had a tendency toward being lower in the SEC group, with good preservation of leukocyte counts, fibrinogen, and antithrombin III. Conclusion: SEC-1 coat™ for cardiopulmonary bypass circuits have good biocompatibility with regard to platelet preservation and in terms of attenuating inflammatory reaction or coagulation activation during pediatric cardiac surgery. It can be beneficial in pediatric as well as adult cardiac surgery.

show abstract

Section: Discussionmentioning

confidence: 99%

Clinical application of a new ternary polymer, SEC-1 coat™, for pediatric cardiopulmonary bypass circuits: a prospective randomized pilot study

et al. 2020

View full text Add to dashboard Cite

show abstract

“…24,25 However, with respect to the membrane oxygenator, several reports have suggested that ACP coating could neutralize cell adhesion and adsorption. 16,26 Based on these reports, significant differences should have been observed Experimental solution comprising albumin, lysozyme, and vancomycin was continuously stirred in a reservoir. In addition, it was continuously circulated in the circuit including a membrane by a roller pump (positioned lower) at a flow rate of 200 mL/min and was continuously filtered by another roller pump (positioned higher) at a flow rate of 50 mL/min.…”

Section: Discussionmentioning

confidence: 99%

“…With regard to adsorption of low-molecular-weight peptide compounds, the vancomycin results suggest two possibilities: ACP coating could inhibit peptide compound adsorption, and in the case of the non-coated membrane, the adsorption would be completed in the initial 15 min. With respect to ACP coating, the absence of protein adsorption has been reported, 16,26 but the adsorption of peptide compounds such as enzymes and/or antibiotics has not been clarified. Vancomycin, a glycopeptide antibiotic with a molecular weight of 1495 Da, has a structure similar to that of enzymes.…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility of a polymer-coated membrane possessing a hydrophilic blood-contacting layer: Adsorption-related assessment

et al. 2019

View full text Add to dashboard Cite

Objective: Currently, the foreign surfaces of extracorporeal circulation devices are coated with an acrylate-based copolymer that creates a hydrophilic blood-contacting layer to enhance biocompatibility. Several reports of acrylate-based copolymer with respect to biocompatibility have been published; however, the adsorption of peptide compounds on acrylate-based copolymer–coated membranes still requires clarity. In this study, we aimed to understand the adsorption of several peptide compounds of various molecular weights, including albumin, lysozyme, and vancomycin, on acrylate-based copolymer–coated membranes using in vitro studies. Methods: Six experimental circuits consisting of acrylate-based copolymer–coated tubes and membranes, and six comprising acrylate-based copolymer–coated tubes and non-coated membranes were prepared for comparison. An experimental solution, composed of albumin, lysozyme, vancomycin, and saline, was continuously stirred in a reservoir, recirculated in each experimental circuit, and then filtered. Concentrations of albumin, lysozyme, and vancomycin were measured after 0, 15, 30, 45, 60, 90, and 120 min of recirculation. Similar experiments were performed in all the prepared circuits. Results: The ratio of measured values at each time point to those at 0 min was not significantly different between acrylate-based copolymer–coated and non-coated membranes for albumin and lysozyme, but differed significantly for vancomycin; the ratios were higher in acrylate-based copolymer–coated than in non-coated membranes. Conclusion: This study suggests that albumin is not adsorbed on either acrylate-based copolymer–coated or non-coated membranes, that lysozyme is not adsorbed on either membrane or is adsorbed at a similar rate on both membranes, and that vancomycin is less adsorbed on acrylate-based copolymer–coated membranes. Thus, acrylate-based copolymer coating could inhibit the adsorption of various peptide compounds.

show abstract

“…The anesthetic and experimental procedures were performed as described previously. 13 In brief, 12 domestic juvenile pigs (body weight = 16 kg) were randomized into two groups: the normothermic (37°C) CPB group and hypothermic (23°C) CPB group (n = 6, respectively). After an overnight fast with free access to water, animals were premedicated by intramuscular injection of ketamine (20 mg/kg), xylazine (3 mg/kg), and 0.5 mg of atropine.…”

Section: Methodsmentioning

confidence: 99%

“…We previously reported that a polymer-coated CPB circuit attenuated upregulation of proteins in both proteases/ protease inhibitors and platelet degranulation. 13 The aim of this study was to verify whether deep hypothermic CPB could reduce upregulation of blood proteins activated by contact with a polymer-coated CPB circuit in juvenile pigs.…”

Section: Introductionmentioning

confidence: 99%

Hypothermic effects on gas exchange performance of membrane oxygenator and blood coagulation during cardiopulmonary bypass in pigs

et al. 2020

Self Cite

View full text Add to dashboard Cite

Introduction: Whether hypothermic cardiopulmonary bypass could attenuate both blood coagulation and platelet activation compared to normothermic cardiopulmonary bypass remains elusive. Methods: Biocompatibility of a polymer-coated cardiopulmonary bypass circuit was comparatively assessed by plasma proteomics between juvenile pigs undergoing hypothermic (23°C) cardiopulmonary bypass and those undergoing normothermic (37°C) cardiopulmonary bypass (n = 6, respectively). Plasma samples were taken three times: 5 minutes after initiation of cardiopulmonary bypass (T5, before cooling), just before declamping and rewarming (Tc), and just before termination of cardiopulmonary bypass (Trw, 120 minutes). Proteomic analysis was quantitively performed by isobaric tags for relative and absolute quantification labeling. Thrombin–antithrombin complexes (TAT III) were measured by enzyme immunoassay, and vitamin K–dependent protein C (PROC), β-thromboglobulin (TG), and P-selectin were measured by enzyme-linked immunosorbent assay. Blood gas analyses evaluated oxygenator performance. Results: Hypothermic cardiopulmonary bypass had a significantly higher PaO2 at Tc and lower PaCO2 at Trw than normothermic cardiopulmonary bypass. Two hundred twenty-four proteins were identified with statistical criteria of both protein confidence (>95%) and false discovery rate (<5%). Six of these proteins significantly decreased at Tc than at T5 in hypothermic cardiopulmonary bypass (p = 0.02-0.04), with three related to platelet degranulation. Protein C decreased at Trw compared with T5 in normothermic cardiopulmonary bypass (p = 0.04). Thrombin–antithrombin complex had a slightly larger increase with normothermic cardiopulmonary bypass at Trw than with hypothermic cardiopulmonary bypass. β-thromboglobulin and P-selectin levels were significantly lower at Trw with hypothermic cardiopulmonary bypass than with normothermic cardiopulmonary bypass (p = 0.04). Conclusion: Hypothermic cardiopulmonary bypass attenuated platelet degranulation/blood coagulation and maintained better oxygenator performance compared to normothermic cardiopulmonary bypass in juvenile pigs.

show abstract

Polymer-coated cardiopulmonary bypass circuit attenuates upregulation of both proteases/protease inhibitors and platelet degranulation in pigs

Abstract: The new polymer (SEC)-coated CPB circuit effectively attenuated upregulation of proteins compared to the non-coated CPB circuit. These proteins were associated with both proteases/protease inhibitors and platelet degranulation.

Cited by 6 publications

References 36 publications

Clinical application of a new ternary polymer, SEC-1 coat™, for pediatric cardiopulmonary bypass circuits: a prospective randomized pilot study

Clinical application of a new ternary polymer, SEC-1 coat™, for pediatric cardiopulmonary bypass circuits: a prospective randomized pilot study

Biocompatibility of a polymer-coated membrane possessing a hydrophilic blood-contacting layer: Adsorption-related assessment

Hypothermic effects on gas exchange performance of membrane oxygenator and blood coagulation during cardiopulmonary bypass in pigs

Contact Info

Product

Resources

About