Polymer Cushions Functionalized with Lipid Molecules

Abstract

This Letter reports a novel approach to the fabrication of a biomimicking surface by modification of an end-functionalizable smooth polymer cushion constructed via chemoselective ligation with a phospholipid-like molecule containing oxyamine groups. The mobility of a phospholipid bilayer formed by vesicle fusion on the phospholipid-like molecule terminated polymer film was characterized by fluorescence recovery after bleaching. Platelet adhesion, as one measure of biocompatibility of the film was also studied and compared to other surfaces such as polyethylene or poly(dimethylsiloxane). The results show that the end-functionalized smooth polymer cushion has potential as a biocompatible platform to reconstitute membrane proteins.

Microscopic wrinkles on supported surfactant monolayers

“…They are related through the constraint (20). Assuming sinusoidal deformation: ξ(x) = h sin(qx), it leads to an expression of L 0 in terms of L, h and q.…”

“…Under the inextensibility constraint (20), we can drop constant terms in expression ( 9) and minimize with respect to the following functional of L 0 and ξ(x):…”

“…The projected length L 0 in x-direction and the surface deformation ξ(x) are not independent variables. They are related through the constraint (20). Assuming sinusoidal deformation: ξ(x) = h sin(qx), it leads to an expression of L 0 in terms of L, h and q.…”

“…Initially these transferred layers are separated from the substrate by a cushion of the carrier liquid. Any topographic patterning of these transferred layers can readily be observed [16,20]. Such patterning is likely affected by interaction with the substrate.…”

Formation and characterization of (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine)/(1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine) supported lipid bilayers on polyelectrolyte multilayer films

“…There have been several examples of such model systems described in the literature including solid SLBs [4,5], polymer-cushioned lipid bilayers [6,7], hybrid bilayers consisting of alkanethiol self-assembled monolayers and lipid monolayers [8][9][10], tethered lipid bilayers with the inner layer of lipid bilayer covalently anchored to a surface through a short spacer group [11,12], suspended lipid bilayers [13,14] or supported vesicular layers [15,16]. Several approaches have been employed to obtain the surface-supported lipid bilayers on hydrophilic substrates.…”