Polymer brush‐grafted monolithic macroporous polyHIPEs obtained by surface‐initiated ARGET ATRP and heparinized for Enterovirus 71 purification

Abstract

Monolithic macroporous polymerized high internal phase emulsions (poly-HIPEs) were grafted with poly(glycidyl methacrylate) (PGMA) brushes via surface-initiated activators continuously regenerated by electron transfer atom transfer radical polymerization (ARGET ATRP) and heparinized for obtaining an affinity chromatographic column for Enterovirus 71 (EV71) purification. The proposed strategy possessed of three advantages. (a) Highly interconnected porous structure of polyHIPEs guaranteed an excellent permeability (9.3 × 10 −14 m 2) and fast mass transfer of biomacromolecules; (b) PGMA brushes provided plentiful epoxy groups for heparin immobilization; (c) High accessibility of heparin was achieved as the polymer brushes could extend into the interior of pores. A specific capture of EV71 was accomplished by receptorligand interaction. Dynamic adsorption capacity for EV71 reached 768 ng per column, up to 5-fold higher than nongrafted one reported in our previous work. The maximum recovery for EV71 was 76.1%. Outstanding adsorption performances were attributed to the high density and accessibility of heparin immobilized on PGMA brushes. In general, the developed polymer brushgrafted polyHIPEs opened up a new broad road for design of novel chromatographic medium for bioseparation and biomedical applications.

Heparinized anticoagulant coatings based on polyphenol-amine inspired chemistry for blood-contacting catheters

“…[11][12][13] These modification approaches could be divided into two categories, one is physical adsorption represented by layer-by-layer self-assembly with the drawback of longterm service stability, [14][15][16][17] and the other is chemical binding via substrate activation or heparin pretreatment associated with a complex process or specific equipment requirements. [18][19][20][21] Of note, the covalent immobilization strategy of heparin activated by 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) has resulted in products reaching the market. 22,23 According to various immobilization methods of heparin, activating inert catheter materials such as polyurethane is a key issue for surface modification.…”

CuO Nanoparticle@Polystyrene Hierarchical Porous Foam for the Effective Encapsulation of Octadecanol as a Phase Changing Thermal Energy Storage Material

“…To overcome these limitations, in addition to PCM microcapsules, − the application of shape-stable porous materials as a PCM carrier has received great interest in the literature. − To date, porous clay, mineral-based materials, metallic foams, porous carbon, and porous polymers , are the most commonly used framework materials for shape-stabilized PCM. High internal phase emulsion templated polymers (PHP) as porous polymers have been used in a wide variety of applications such as adsorption, , purification, , separation, controlled release, , and scaffolding, and there is limited research in energy storage application. , Recently, porous PHP foams have begun to gain attention for the stabilization of PCMs owing to their superior features such as shape stabilization, enclosure ability, high loading rate, excellent structural stability, and interpenetrating pore morphology. − PHPs are monolithic, controllable, highly porous polymers with low density, high specific surface area, highly cross-linked polymer matrix, the porosity of which is created by the continuous phase of emulsions. PHPs in particular stand out for their large gaps bounded by small windows on the skeleton.…”