Polymer Thin Film–Induced Tumor Spheroids Acquire Cancer Stem Cell–like Properties

Choi, Minsuk; Yu, Seung Jung; Choi, Yuni; Lee, Hak Rae; Lee, Eunbeol; Lee, Eunjung; Song, Jun; Son, Jin Gyeng; Lee, Tae G.; Kim, Jinyong; Kang, Sukmo; Baek, Jieung; Im, Sung Gap; Jon, Sangyong

doi:10.1158/0008-5472.can-18-0927

Cited by 23 publications

(24 citation statements)

References 53 publications

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“…Culturing of human cancer stem cells to form multicellular tumor spheriods was readily, and reproducable achieved on crosslinked iCVD polysiloxane surfaces. 152 The resulting spheroids are desired for drug screening and efficacy testing.…”

Section: Biotechnologymentioning

confidence: 99%

Nanoscale control by chemically vapour-deposited polymers

Gleason

2020

Nat Rev Phys

104

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Section: Biotechnologymentioning

confidence: 99%

Nanoscale control by chemically vapour-deposited polymers

Gleason

2020

Nat Rev Phys

104

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“…3 (D to H) and 4K and figs. S10D and S16 (H and I), we used our in-house developed poly(2,4,6,8tetravinyl-2,4,6,8-tetramethl cyclotetrasiloxane) (pV4D4) polymer thin film-based scaffold culture plates (30). The thickness of pV4D4 films on plates was monitored in situ using a He-Ne laser (JDS Uniphase) interferometer system before use.…”

Section: D Culture-based Assaysmentioning

confidence: 99%

Prior acquired resistance to paclitaxel relays diverse EGFR-targeted therapy persistence mechanisms

Aldonza

Hong

et al. 2020

Sci. Adv.

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Secondary drug resistance stems from dynamic clonal evolution during the development of a prior primary resistance. This collateral type of resistance is often a characteristic of cancer recurrence. Yet, mechanisms that drive this collateral resistance and their drug-specific trajectories are still poorly understood. Using resistance selection and small-scale pharmacological screens, we find that cancer cells with primary acquired resistance to the microtubule-stabilizing drug paclitaxel often develop tolerance to epidermal growth factor receptor–tyrosine kinase inhibitors (EGFR-TKIs), leading to formation of more stable resistant cell populations. We show that paclitaxel-resistant cancer cells follow distinct selection paths under EGFR-TKIs by enriching the stemness program, developing a highly glycolytic adaptive stress response, and rewiring an apoptosis control pathway. Collectively, our work demonstrates the alterations in cellular state stemming from paclitaxel failure that result in collateral resistance to EGFR-TKIs and points to new exploitable vulnerabilities during resistance evolution in the second-line treatment setting.

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“…Dissociated tumor cells exhibit a tendency to generate organized multi-cellular structures termed “spheroids”, disorganized “cellular aggregates”, or they exist as single cells [37]. Cell aggregates and spheroids exhibit stem-like and anoikis resistance properties, and are associated with disease aggressiveness [38,39].…”

Section: Snapshot Assays For Metastasis Assessmentmentioning

confidence: 99%

Uncoupling Traditional Functionalities of Metastasis: The Parting of Ways with Real-Time Assays

Varankar

Bapat

2019

JCM

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The experimental evaluation of metastasis overly focuses on the gain of migratory and invasive properties, while disregarding the contributions of cellular plasticity, extra-cellular matrix heterogeneity, niche interactions, and tissue architecture. Traditional cell-based assays often restrict the inclusion of these processes and warrant the implementation of approaches that provide an enhanced spatiotemporal resolution of the metastatic cascade. Time lapse imaging represents such an underutilized approach in cancer biology, especially in the context of disease progression. The inclusion of time lapse microscopy and microfluidic devices in routine assays has recently discerned several nuances of the metastatic cascade. Our review emphasizes that a complete comprehension of metastasis in view of evolving ideologies necessitates (i) the use of appropriate, context-specific assays and understanding their inherent limitations; (ii) cautious derivation of inferences to avoid erroneous/overestimated clinical extrapolations; (iii) corroboration between multiple assay outputs to gauge metastatic potential; and (iv) the development of protocols with improved in situ implications. We further believe that the adoption of improved quantitative approaches in these assays can generate predictive algorithms that may expedite therapeutic strategies targeting metastasis via the development of disease relevant model systems. Such approaches could potentiate the restructuring of the cancer metastasis paradigm through an emphasis on the development of next-generation real-time assays.

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Polymer Thin Film–Induced Tumor Spheroids Acquire Cancer Stem Cell–like Properties

Cited by 23 publications

References 53 publications

Nanoscale control by chemically vapour-deposited polymers

Nanoscale control by chemically vapour-deposited polymers

Prior acquired resistance to paclitaxel relays diverse EGFR-targeted therapy persistence mechanisms

Uncoupling Traditional Functionalities of Metastasis: The Parting of Ways with Real-Time Assays

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