Polymerase chain reaction detection of <i> Treponema denticola</i> in endodontic infections within root canals

Siqueira, José F.; Rôças, Isabela N.; Favieri, Amauri; Oliveira, Julio Cezar M.; Santos, Kátia Regina Netto dos

doi:10.1046/j.1365-2591.2001.00382.x

Cited by 46 publications

(40 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We detected this species in 40% of the samples, but in low levels. Similarly to our data, Siqueira et al (24) and Baumgartner et al (25) also reported a high prevalence of spirochetes in samples from endodontic infections using a different molecular method. Thus, it seems that these difficult-to-grow microorganisms have been underestimated in the past, as well as their role in the etiology of endodontic infections.…”

Section: Discussionsupporting

confidence: 91%

Endodontic Therapy Associated with Calcium Hydroxide As an Intracanal Dressing: Microbiologic Evaluation by the Checkerboard DNA-DNA Hybridization Technique

Souza

Teles

Souto

et al. 2005

Journal of Endodontics

View full text Add to dashboard Cite

This study evaluated the predominant microbiota of infected necrotic pulps and the effects of calcium hydroxide therapy on these microorganisms by the checkerboard DNA-DNA hybridization technique. Conventional endodontic therapy associated with calcium hydroxide as intracanal dressing was performed in 12 single-rooted teeth with pulp necrosis and periradicular bone lesion. Samples were collected from the canal at baseline and 14 days after therapy, and the presence of 44 bacterial species was determined by the checkerboard method. Significant differences in the microbiota from baseline to post-therapy were sought by the paired-samples t test. The most prevalent species included F. nucleatum ss. vincentii, C. sputigena, C. ochracea, S. constellatus, V. parvula, P. gingivalis, P. melaninogenica, and S. sanguis. Most of the microorganisms were reduced after treatment, particularly A. gerencseriae, A. israelii, A. naeslundii, C. gingivalis, C. ochracea, P. gingivalis, S. noxia, S. sanguis, and S. oralis (p Ͻ 0.05). Conversely, A. actinomycetemcomitans, C. sputigena, and E. corrodens increased in numbers after therapy. These results indicate that conventional endodontic therapy with calcium hydroxide results in the reduction of pathogenic species associated with pulp necrosis. However, its use is limited, because it did not eliminate the whole spectrum of microorganisms.

show abstract

Section: Discussionsupporting

confidence: 91%

Endodontic Therapy Associated with Calcium Hydroxide As an Intracanal Dressing: Microbiologic Evaluation by the Checkerboard DNA-DNA Hybridization Technique

Souza

Teles

Souto

et al. 2005

Journal of Endodontics

View full text Add to dashboard Cite

show abstract

“…Many of the species that are reported as new are split off from previously established genera and species, but the ease of identifying culturedifficult species and the specificity of PCR-based methods has meant that some additional species can be included as typical of the microbial flora of the infected root canal. These include spirochaetes, [31][32][33][34][35] and the species Tanerella forsythensis (formerly Bacteroides forsythus), [36][37][38][39][40] which are prevalent in infected root canals yet difficult to cultivate.…”

Section: Contribution Of Molecular Techniquesmentioning

confidence: 99%

A big role for the very small—understanding the endodontic microbial flora

Figdor

Sundqvist

2007

Australian Dental Journal

View full text Add to dashboard Cite

Apical periodontitis, an inflammatory process around the apex of a tooth root, is primarily a sequel to microbial infection of the pulp space. The microbial flora is composed of a restricted group of the total oral flora, selected by environmental pressures of anaerobiosis, nutrition and competition with other species and inhabits the root canal as a biofilm of coaggregated communities in an extracellular matrix. The untreated infected canal is generally composed of a polymicrobial mix with approximately equal proportions of Gram-positive and Gram-negative species, dominated by obligate anaerobes. The type of microbial flora in the root-filled tooth with persistent apical periodontitis has very different characteristics. These infections are characterized by one or just a few species, predominantly Grampositive micro-organisms with an equal distribution of facultative and obligate anaerobes. Enterococcus faecalis has been a conspicuous finding in most studies. Because the primary aetiological problem is infection, endodontic treatment is directed at control and elimination of the root canal flora by working in a sterile way. Based on current knowledge, the best available method for obtaining clean, microbe-free root canals is by instrumentation with antimicrobial irrigation reinforced by an intracanal dressing with calcium hydroxide.Key words: Root canal flora, ecological niche, antibacterial treatment, root canal preparation, root canal irrigants.Abbreviations and acronyms: EDTA = ethylenediamine tetra-acetic acid; FTM = fluid thioglycolate medium; NaOCI = sodium hypochlorite solution; SEM = scanning electron microscope.

show abstract

“…이 같은 배양법을 보완, 보충하기위해 1990년대 이후 다양한 분자 유전학적기술, 즉 checkerboard DNA:DNA hybridization 25) , real time-PCR 26) , 16S rRNA(DNA)gene-directed PCR 27,28) 등을 34,44) . 브라질에서 감염근관을 대상으로 16S rRNA genedirected PCR방법으로 조사한 결과 T. denticola는 임상 증상과 연관성있게 나타나는 것은 아니지만 임상증상 유무 에 관계없이 살펴봤을 때 42�52%의 높은 이환율을 보였 다 [33][34][35] . 그러나 국내에서 감염근관을 조사한 Jung 등 30) .…”

unclassified

“…Distribution of bacteria in fistula*; Samples were obtained through the opening of the fistula, whereas the others from the infected the root canals. [33][34][35][36]37) . Table 5.…”

mentioning

confidence: 99%

“…Distribution of bacteria in necrotic root canal samples of apical lesions without clinical symptoms T. denticola는 임상증상의 존재유무와 관계없이 거의 일 정하게 50%전후하는 이환율을 보이고 있으며 [33][34][35] , 이 이환 율은 본 연구의 것(52.8%)과 거의 일치하는 것으로 나타났 다. T. denticola는 이전의 배양법에 기초한 분포조사에서 는 거의 검출되지 않았으나 최근 분자생물학적, 유전학적 방법을 이용하여 발견되면서 증상과 관련되어 있을 수 있다 는 새로운 가능성 때문에 주목을 받고 있다. T. denticola는 증상에 관계없이 어느 곳에서도 나타나지만 33,35) , 특별히 중 요한 근관감염의 세균인 P. gingivalis와 강하게 응집반응 을 하여 47) 인접한 상태에서 상호 필요한 영양분을 공급하는 공생관계를 이루고 있다 48) . 이와 같은 이유로 치주염 이환부 위에서는 T. dentidola가 언제나 P. gingivalis와 같이 나 타나는 것으로 보고되고 있다 49) .…”

unclassified

See 1 more Smart Citation

Distribution of oral pathogens in infections of endodontic origin

Kim

Choi

Park

et al. 2003

J Korean Acad Conserv Dent

View full text Add to dashboard Cite

It has been documented that periodontopathic bacteria are also implicated in endodontic infections. 16S rDNA gene-directed PCR was to examine the prevalence of periodontopathic bacteria including Actinobacillus actinomycetemcomitans (Aa), Prevotella intermedia (Pi), Prevotella nigrescens (Pn), Porphyromonas gingivalis (Pg), Porphyromonas endodontalis (Pe), and Treponema denticola (Td) in the root canals of 36 endodontically infected teeth having apical lesions with or without clinical symptoms like pain, swelling, and fistula.1. In 36 infected root canals, most frequently detected bacterial species was Pg (61.1%), followed by Td (52.8%) and Pe (38.9%). 2. Of 36 infected root canals, Aa was detected in 6 canals (16.7%) of the teeth, all of which showed clinical symptoms. 3. Of 36 infected root canals, Pi and Pn were found in 4 (13.9%) and 5 (33.3%), respectively. Notably, prevalence of Pn in the symptomatic teeth was 50.0%. 4. One of black-pigmented anaerobic bacteria (BPB) including Pi, Pn, Pe, and Pg was detected in all of the teeth that showed pain or especially swelling but not fistula. It was, however, found that prevalence of BPB in the asymptomatic teeth or the teeth with fistula was only 40%. 5. Pe and Pg were detected in the teeth regardless of the presence or absence of symptoms. 6. Td was detected in the teeth regardless of the presence or absence of symptoms. High prevalence of BPB in the symptomatic teeth but low in the asymptomatic teeth suggests that BPB may play an important role in the pathogenesis of periapical lesions.K Ke ey y w wo or rd ds s : PCR, BPB, infection, clinical symptom, periapical lesion, root canal ABSTRACT 치료의 성공을 좌우한다

show abstract

Polymerase chain reaction detection of Treponema denticola in endodontic infections within root canals

Cited by 46 publications

References 27 publications

Endodontic Therapy Associated with Calcium Hydroxide As an Intracanal Dressing: Microbiologic Evaluation by the Checkerboard DNA-DNA Hybridization Technique

Endodontic Therapy Associated with Calcium Hydroxide As an Intracanal Dressing: Microbiologic Evaluation by the Checkerboard DNA-DNA Hybridization Technique

A big role for the very small—understanding the endodontic microbial flora

Distribution of oral pathogens in infections of endodontic origin

Contact Info

Product

Resources

About