Polymethacrylate Coated Electrospun PHB Fibers as a Functionalized Platform for Bio-Diagnostics: Confirmation Analysis on the Presence of Immobilized IgG Antibodies against Dengue Virus

Abstract

In this article, a combination of far field electrospinning (FFES) and free-radical polymerization has been used to create a unique platform for protein immobilization via the physical attachment of biomolecules to the surface of the fiber mats. The large specific surface area of the fibers with its tailored chemistry provides a desirable platform for effective analyte-surface interaction. The detailed analysis of protein immobilization on a newly developed bio-receptive surface plays a vital role to gauge its advantages in bio-diagnostic applications. We relied on scanning electron microscopy (SEM), diameter range analysis, and X-ray photoelectron spectroscopy (XPS), along with thermal gravimetric analysis (TGA), water-in-air contact angle analysis (WCA), Fourier transform infrared spectroscopy (FTIR), and atomic force microscopy (AFM) to study our developed platforms and to provide valuable information regarding the presence of biomolecular entities on the surface. Detailed analyses of the fiber mats before and after antibody immobilization have shown obvious changes on the surface of the bioreceptive surface including: (i) an additional peak corresponding to the presence of an antibody in TGA analysis; (ii) extra FTIR peaks corresponding to the presence of antibodies on the coated fiber platforms; and (iii) a clear alteration in surface roughness recorded by AFM analysis. Confirmation analyses on protein immobilization are of great importance as they underlay substantial grounds for various biosensing applications.

Functional electrospun polymeric materials for bioelectronic devices: a review

“…This fiber-based sensor system achieved a detection sensitivity of 97% in comparison to the clinical method, with a detection sensitivity of around 76%. This improvement was achieved due to the electrospun fibers with the high surface area providing opportunities for more controlled surface modification and biomolecular interaction for dengue virus detection [57,58]. Fiber-based enzyme-linked immunosorbent assay (ELISA) systems were 10 developed using electrospun PLLA and CA substrates to detect the C-reactive protein (CRP) as a cardiac biomarker.…”

Optical sensors based on electrospun membranes – principles, applications, and prospects for chemistry and biology

“…First, pieces of a membrane can be placed into the wells of a plate for further analysis with the conventional readers. [69][70][71] Second, they can be used in macroscopic flow-through systems. [72][73][74][75] Finally, EMs can be used as parts of LFA [76][77][78][79][80][81] or microfluidic devices.…”

“…Then, labeled detection molecules are introduced, usually as conjugates with a fluorescent label (for the fluorescence detection) or with the Au NPs (for the colorimetric detection) or with the horseradish peroxidase (HRP, for either chemiluminescence or colorimetric detection, depending on the substrate). With minimal modifications, this scheme can be applied for large analytes, such as viruses 69,70,130 or bacterial cells. 77 Thus, the general scheme of label-based biological OSBEMs is shown in Fig.…”

“…The ultimate LOD achieved with such a system was 8 PFU mL À1 . 69,70 For comparison, the commercially available RT-PCR assays usually require B10 RNA copies per reaction, and the reaction volume is on the order of B100 mL.…”

Electrospun Biopolyesters: Hydrophobic Scaffolds with Favorable Biological Response

“…1.b, θ ~120°). This behavior is mainly due to the chemical structure of the biopolyesters and the absence of hydrophilic functional groups as well as relative roughness of the electrospun surfaces, and the air trapped in the micropores of fibrous network [18].…”