Polymethoxyflavones from Gardenia oudiepe (Rubiaceae) induce cytoskeleton disruption-mediated apoptosis and sensitize BRAF-mutated melanoma cells to chemotherapy

Oliveira-Júnior, Raimundo Gonçalves de; Marcoult-Fréville, Nolwenn; Prunier, Grégoire; Beaugeard, Laureen; Filho, Edilson Beserra de Alencar; Mourão, Eduard David Simões; Michel, Sylvie; Quintans‐Júnior, Lucindo José; Almeida, Jackson Roberto Guedes da Silva; Grougnet, Raphaë͏l; Picot, Laurent

doi:10.1016/j.cbi.2020.109109

Cited by 9 publications

(9 citation statements)

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“…A2058 cells are found to be more sensitive to 14/15 than to vemurafenib (IC 50 = 5.93 μM). 27 If chemotherapy is applied for the treatment of non-melanoma skin cancers, 5-fluorouracil ( e.g. in the form of its oral prodrug capecitabine or in creams) may be used.…”

Section: Resultsmentioning

confidence: 99%

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Synthesis, structure and in vitro antiproliferative effects of alkyne-linked 1,2,4-thiadiazole hybrids including erlotinib- and ferrocene-containing derivatives

et al. 2021

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Section: Resultsmentioning

confidence: 99%

“…This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. to 14/15 than to vemurafenib (IC 50 ¼ 5.93 mM) 27. If chemotherapy is applied for the treatment of non-melanoma skin cancers, 5-uorouracil (e.g.…”

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confidence: 99%

Synthesis, structure and in vitro antiproliferative effects of alkyne-linked 1,2,4-thiadiazole hybrids including erlotinib- and ferrocene-containing derivatives

et al. 2021

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“…Because actin is a structural protein of microfilaments and a major cytoskeletal protein, it plays an important role in many subcellular processes, such as cytoplasmic flow, organelle and nuclear localization, cell morphogenesis and cell division [ 36 ]. Similarly, microtubules, as a polar cytoskeleton, mainly play a role in maintaining cell morphology and assisting intracellular transport, and can be assembled with other proteins into spindles, grana, centrioles, flagella, ciliated neural tubes and other structures [ 37 , 38 ]. The observation of microfilaments and microtubules by LSCM can determine whether the structure of cellular protein fiber network is still intact, and whether the maintenance of morphology, cell movement, intracellular material transport and cell division are normal.…”

Section: Discussionmentioning

confidence: 99%

Effect of Silkworm Pupa Protein Hydrolysates on Proliferation of Gastric Cancer Cells In Vitro

Zou

et al. 2022

Foods

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The proliferation inhibition effects of the hydrolysates from silkworm pupa proteins on MGC-803 gastric cancer cells were investigated in this study. The specific morphological changes (cell membrane, cell nucleus and cytoskeleton) of cells were measured. In vitro, the proliferation of MGC-803 cells was inhibited by silkworm pupa protein hydrolysates (SPPHs) in a dose-dependent manner. The flow cytometry analysis showed that the blocking effect of SPPHs on the MGC-803 cells was mainly in the G0/G1-phase. The morphological changes, disintegration of the cytoskeleton and retardant cell cycles were probably related to the activation of apoptosis. Thus, SPPHs could be promising as a chemopreventive agent due to their ability to promote apoptosis of tumor cells.

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“…As a traditional Chinese medicine extracted form Xanthoceras sorbifolia , Toosendan fructus , Gardeniae fructus , and Chebulae fructus , monomers isolated from these herbs have been documented with roles in antitumor effect. For example, recently study reported that polymethoxyflavones from Gardenia oudiepe (Rubiaceae) induce cytoskeleton disruption-mediated apoptosis and sensitize BRAF-mutated melanoma cells to chemotherapy [ 38 ]. In addition, antitumor effects have also been reported by other group that the ethanol extract of baked Gardeniae fructus exhibits in vitro and in vivo antimetastatic and antiangiogenic activities in malignant cancer cells by suppression of the NF- κ B and HIF-1 α pathways [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Sendeng-4 Suppressed Melanoma Growth by Induction of Autophagy and Apoptosis

Zhao

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Sendeng-4 is a traditional Chinese medicine that has been successfully applied to anti-inflammatory diseases in clinical practice. Monomers within Sendeng-4 showed promising antitumor activity against lung cancer, colon cancer, and cutaneous cancer. However, potency of Sendeng-4 in melanoma has not been explored. This study aims to explore the potential application of Sendeng-4 in melanoma treatment. In the present study, we systemically investigate the possibility of Sendeng-4 for treatment of melanoma cancer in vitro by proliferation assay, colony formation, flow cell cytometry, RNA-seq, western blot, and fluorescence-based assay. Our data demonstrated that Sendeng-4 suppresses the proliferation and colony formation capacity of melanoma cells and induces cell cycle block at G2/M phase and eventually cell death. Mechanistically, transcriptome sequencing demonstrates that the PI3K-AKT pathway was significantly inactivated upon Sendeng-4 exposure, which was confirmed by western blot showing decreased phosphorylation of AKT. In addition, decreased BCL-2 expression and increased BAX expression were observed, suggesting programmed cell death via apoptosis. Moreover, LC3-II production as well as autophagosomes formation was observed as demonstrated by western blot and immunofluorescence, indicating elevated autophagy network by Sendeng-4 stimulation. Collectively, we concluded that Sendeng-4 might be used as an anticancer drug for melanoma.

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Polymethoxyflavones from Gardenia oudiepe (Rubiaceae) induce cytoskeleton disruption-mediated apoptosis and sensitize BRAF-mutated melanoma cells to chemotherapy

Cited by 9 publications

References 60 publications

Synthesis, structure and in vitro antiproliferative effects of alkyne-linked 1,2,4-thiadiazole hybrids including erlotinib- and ferrocene-containing derivatives

Synthesis, structure and in vitro antiproliferative effects of alkyne-linked 1,2,4-thiadiazole hybrids including erlotinib- and ferrocene-containing derivatives

Effect of Silkworm Pupa Protein Hydrolysates on Proliferation of Gastric Cancer Cells In Vitro

Sendeng-4 Suppressed Melanoma Growth by Induction of Autophagy and Apoptosis

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