Polymorphic microsatellite markers for studies of <i> Aedes aegypti</i> (Diptera: Culicidae), the vector of dengue and yellow fever

Slotman, Michel A.; Kelly, N. B.; Harrington, Laura C.; Kitthawee, Sangvorn; Jones, James W.; Scott, Thomas W.; Caccone, Adalgisa; Powell, Jeffrey R.

doi:10.1111/j.1471-8286.2006.01533.x

Cited by 83 publications

(102 citation statements)

References 11 publications

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“…However, it is extremely unlikely that a male would pass one of its alleles to only one out of 15 offspring, given that this locus was initially found to be in Hardy-Weinberg Equilibrium (Supplemental Table 2). 31 Instead, it is more probable that two fathers inseminated the mother, with one contributing to 14 offspring, and the other contributing to a single offspring.…”

Section: Resultsmentioning

confidence: 99%

Evidence of Limited Polyandry in a Natural Population of Aedes aegypti

Richardson¹,

Jameson²,

Gloria‐Soria³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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Abstract. The mosquito Aedes aegypti is a vector of yellow fever, dengue, and chikungunya. Control of the insect is crucial to stop the spread of dengue and chikungunya, so it is critically important to understand its mating behavior. Primarily, based on laboratory behavior, it has long been assumed that Ae. aegypti females mate once in their lifetime. However, multiple inseminations have been observed in semi-field and laboratory settings, and in closely related species. Here, we report the first evidence of polyandry in a natural population of Ae. aegypti. Female Ae. aegypti were captured around the New Orleans, LA, metropolitan area. They were offered a blood meal and allowed to lay eggs, which were reared to the third-instar larval stage. A parentage analysis using four microsatellite loci was performed. Out of 48 families, 3 showed evidence of multiple paternity. An expanded analysis of these three families found that one family group included offspring contributed by three fathers, and the other two included offspring from two fathers. This result establishes that polyandry can occur in a small proportion of Ae. aegypti females in a natural setting. This could complicate future genetic control efforts and has implications for sampling for population genetics.

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Section: Resultsmentioning

confidence: 99%

Evidence of Limited Polyandry in a Natural Population of Aedes aegypti

Richardson¹,

Jameson²,

Gloria‐Soria³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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“…This consisted of 2 ml of 43 diluted genomic DNA; 0.3 ml [g 33 P]ATP-end-labeled forward primer, 1 mm; 0.1 ml unlabeled forward primer, 10 mm; 0.4 ml reverse primer, 10 mm; 2 ml dNTPs (Bioline), 1 mm; 0.5 ml bovine serum albumin (New England Biolabs, Ipswich, MA), 1%; 1 ml 103 PCR buffer with 2.0 mm Mg 21 ; 0.4 unit of Taq polymerase (New England Biolabs); and 3.62 ml sterile water. PCR specifications are based on previous studies (Chambers et al 2007;Slotman et al 2007;Endersby et al 2009). Thirty females per line were genotyped.…”

Section: Methodsmentioning

confidence: 99%

“…Two EPIC markers (Endersby et al 2009) and six microsatellites (Chambers et al 2007;Slotman et al 2007;Endersby et al 2009) were used to study variation in the backcrosses (see Endersby et al 2009). PCR was performed as a 10-ml reaction per individual.…”

Section: Methodsmentioning

confidence: 99%

Dynamics of the “Popcorn” Wolbachia Infection in Outbred Aedes aegypti Informs Prospects for Mosquito Vector Control

et al. 2011

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Forty percent of the world's population is at risk of contracting dengue virus, which produces dengue fever with a potentially fatal hemorrhagic form. The wMelPop Wolbachia infection of Drosophila melanogaster reduces life span and interferes with viral transmission when introduced into the mosquito Aedes aegypti, the primary vector of dengue virus. Wolbachia has been proposed as an agent for preventing transmission of dengue virus. Population invasion by Wolbachia depends on levels of cytoplasmic incompatibility, fitness effects, and maternal transmission. Here we characterized these traits in an outbred genetic background of a potential target population of Ae. aegypti using two crossing schemes. Cytoplasmic incompatibility was strong in this background, and the maternal transmission rate of Wolbachia was high. The infection substantially reduced longevity of infected adult females, regardless of whether adults came from larvae cultured under high or low levels of nutrition or density. The infection reduced the viability of diapausing and nondiapausing eggs. Viability was particularly low when eggs were laid by older females and when diapausing eggs had been stored for a few weeks. The infection affected mosquito larval development time and adult body size under different larval nutrition levels and densities. The results were used to assess the potential for wMelPop-CLA to invade natural populations of Ae. aegypti and to develop recommendations for the maintenance of fitness in infected mosquitoes that need to compete against field insects.

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“…However, in contrast to most other arthropods (e.g., Anopheles gambiae s. 58 ), microsatellites appear to be underrepresented within some members of the mosquito subfamily Culicinae (e.g., Culex pipiens, C. pipiens quinquefasciatus, and A. aegypti [59][60] ). Nevertheless, in A. aegypti for instance, microsatellites are commonly used in population genetics studie 61 .…”

Section: Introductionmentioning

confidence: 94%

How much is known about the genetic diversity of the Asian tiger mosquito? A systematic review

Aguirre-Obando

Navarro-Silva

2017

revsal

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Introduction: Aedes (Stegomyia) albopictus (Skuse, 1894) is a vector for dengue and chikungunya viruses in the field, along with around 24 additional arboviruses under laboratory conditions. Knowledge of the genetic diversity of insect vectors is critical for the effective control and elimination of vector-borne diseases. Objective: We determined the current scenario of the genetic diversity in natural populations of A. albopictus through a systematic review. Methodology: It was possible to establish the first reports and distribution of A. albopictus populations in the world, as well as its genetic diversity, population genetic structure and molecular markers used to determine its genetic diversity. Results: A. albopictus is distributed worldwide with genetically structured populations and low diversity; however, 89.5% of the genetic diversity known is based on the use of RFLP, allozymes, isozymes, and mtDNA molecular markers that exhibit significant problems according to the literature. After the results were obtained, a critical analysis was carried out and existing shortcomings were detected. Conclusion: The current knowledge of genetic diversity of A. albopictus is based on genetic markers that exhibit significant problems reported in the literature; therefore, vector control programs targeting A. albopictus populations, may be compromised.

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Polymorphic microsatellite markers for studies of Aedes aegypti (Diptera: Culicidae), the vector of dengue and yellow fever

Cited by 83 publications

References 11 publications

Evidence of Limited Polyandry in a Natural Population of Aedes aegypti

Evidence of Limited Polyandry in a Natural Population of Aedes aegypti

Dynamics of the “Popcorn” Wolbachia Infection in Outbred Aedes aegypti Informs Prospects for Mosquito Vector Control

How much is known about the genetic diversity of the Asian tiger mosquito? A systematic review

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