Polymorphism in XRCC1, XRCC2, XRCC3 Genes and Risk of Gastrointestinal Cancer: A Case-Control Study from South-Western Maharashtra

Abstract

Background: Alarming increased incidence of gastrointestinal (GI) cancer in rural parts of Maharashtra intended us to elucidate association of polymorphism in DNA repair genes with GI cancer risk.Objective: Hospital based case-control study designed to investigate association of polymorphisms in XRCC1, XRCC2, XRCC3 genes with risk of GI in Maharashtrian population.Method: PCR-RFLP method was used to genotype polymorphisms in exon 6, 9 and 10 of XRCC1, exon 3 of XRCC2 and exon 7 of XRCC3 gene from 200 GI cancer patients and 300 controls. Results:The genotypic frequency of variant allele His/His of cd280 of XRCC1 showed association with increased risk of GI cancer as compared to Arg/Arg genotype in rural population (OR = 14.04; 95% CI: 9.05-21.78, p<0.0001). The results of XRCC2 interpreted negative association (OR = 0.27; 95% CI: 0.10-0.73, p=0.006) and no association of XRCC3 (OR = 0.20; 95% CI: 0.08-0.53, p=0.004) with GI cancer. Conclusion:The findings from this study revealed possible association of XRCC1 codon 280 polymorphism with increased risk of GI carcinogenesis in rural population of Maharashtra.