Polymorphism of PCR profiles and expression of alleles at the locus Adh1 in agamospermous progeny of sugar beet Beta vulgaris L.

Molecular differences in genotroph forms of common wheat (Triticum aestivum L.) and their initial cultivars

“…For revelation of polymorphism of PCR-profiles of wheat plants the modified ISSR-amplification method was used. In this case in the pair with the microsatellite primer the specific primer was used [13][14][15]. Using available in Internet DNA sequences of wheat we selected primers for enzyme loci controlling: alcohol dehydrogenase (ADH1), malic-enzyme (ME1) and glutamate oxaloacetate transaminase (GOT1).…”

“…To study DNA structure, the modified ISSR-amplification method is successfully used. In this case in the pair with the specific to marker enzyme gene primer, the microsatellite primer is used [13][14][15]. Due to this method which allows researching the DNA-sites including the marker enzyme gene, the differences between plants carrying the same allele of enzyme locus and also the differences in marker enzyme locus structure revealed in different tissues of the same plant are shown [13].…”

Changes in the organization of isozyme loci Me1 and Adh1 in-duced with triton X-100 in common wheat lines

“…To study the DNA structure, a modified ISSR-amplification method is convenient where in a pair with a specific isozyme gene primer microsatellite one is used [17][18][19]. By means of this method differences among plants carrying the same allele of isozyme locus were shown and also differences in the structure of marker isozyme locus evincing in different tissues of the same plant were revealed [19].…”